tantan-22/0000755000000000000000000000000013406427735011275 5ustar rootroottantan-22/test/0000755000000000000000000000000013406427735012254 5ustar rootroottantan-22/test/tantan_test.sh0000755000000000000000000000154713406427735015146 0ustar rootroot#! /bin/sh # Run tantan with various inputs and check the outputs. cd $(dirname $0) # Make sure we use this version of tantan: PATH=../src:$PATH countLowercaseLetters () { grep -v '^>' "$@" | tr -cd a-z | wc -c | tr -d ' ' } { tantan hg19_chrM.fa echo tantan -p -xx titin_human.fa echo tantan -p -f2 titin_human.fa echo tantan -c hg19_chrM.fa | countLowercaseLetters echo tantan -w50 -e.005 -d1 hg19_chrM.fa | countLowercaseLetters echo tantan -m atMask.mat -r.01 hg19_chrM.fa | countLowercaseLetters echo tantan -p -a11 -b2 titin_human.fa | countLowercaseLetters echo tantan panda.fastq echo tantan -i2 -j3 hg19_chrM.fa | countLowercaseLetters echo tantan -f4 panda.fastq echo tantan -f4 -b12 hard.fa echo tantan -f4 -n1 hg19_chrM.fa } 2>&1 | diff -u tantan_test.out - tantan-22/test/atMask.mat0000644000000000000000000000077213406427735014205 0ustar rootroot# This scoring matrix targets alignments with the following properties. # Percent identity: 92.1563 # Percent A+T: 78.935 # Ratio of transitions to transversions: 0.332553 # Ratio of transition rate to transversion rate (alpha/beta): 1 # Expected number of transitions per site: 0.0208262 # Expected number of transversions per site: 0.0626253 # Expected number of substitutions per site: 0.0834515 # Scale parameter (lambda): 0.427882 a c g t a 2 -5 -5 -5 c -5 5 -5 -5 g -5 -5 5 -5 t -5 -5 -5 2 tantan-22/test/tantan_test.out0000644000000000000000000022166013406427735015340 0ustar rootroot>chrM GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCAT TTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTG GAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATT CTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTA AAGTGTGTTAATtaattaaTGCTTGTAGGACATAATAATAACAATTGAAT GTCTGCACAGCCGCTTTCCACACAGACATCATAACAaaaaatttccacca aacccccccctccccccGCTTCTGGCCACAGCACTTAAACACATCTCTGC CAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAAT TTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACA TTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCC GCCCATCCTACCCAGCACacacacaCCGCTGCTAACCCCATACCCCGAAC CAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCA AAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAA ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC AAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAG GGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCC ACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGT TTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACC GCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTT TAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAA CTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAAC ACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGC CCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGA GCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGA GGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGC TCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGT AAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGG TGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTT ATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGT GCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCC TCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATA GAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAaagtgcacTTGGACG AACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTT CAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCAC CTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGG CGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGA TGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTT CTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAA GACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCC GTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCT ACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTT TAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAG TCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGA GTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGA AAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACT GAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAA TGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCA GATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA ACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAG GAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAACCTTACCCCGCCTGTT TACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCC CAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCA TAATCACTTGTTCCTTAAATAGGGACCTGTATGAATGGCTCCACGAGGGT TCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGG CGGGCATGACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTA ATGCAAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCA TTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAacctccgagca gtaCATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT CCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATC CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGAT CAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA TTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTT CTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAGGCC TACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTAT TATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGG TAATCGCATAAAACTTAaaactTTACAGTCAGAGGTTCAATTCCTCTTCT TAACAACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAA TCGCAATGGCATTCCTAATGCTTACCGAACGAAAAATTCTAGGCTATATA CAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTACAACC CTTCGCTGACGCCATAAAACTCTTCACCAAAGAGCCCCTAAAACCCGCCA CATCTACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTCACCATC GCTCTTCTACTATGAACCCCCCTCCCCATACCCAACCCCCTGGTCAACCT CAACCTAGGCCTCCTATTTATTCTAGCCACCTCTAGCCTAGCCGTTTACT CAATCCTCTGATCAGGGTGAGCATCAAACTCAAACTACGCCCTGATCGGC GCACTGCGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCAT CATTCTACTATCAACATTACTAATAAGTGGCTCCTTTAACCTCTCCACCC TTATCACAACACAAGAACACCTCTGATTACTCCTGCCATCATGACCCTTG GCCATAATATGATTTATCTCCACACTAGCAGAGACCAACCGAACCCCCTT CGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGCTTCAACATCGAAT ACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATACACAAACATT ATtataataAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGA CGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTAC TTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCCGC TACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCT AGCATTACTTATATGATATGTCTCCATACCCATTACAATCTCCAGCATTC CCCCTCAAACCTAAGAAATATGTCTGATAAAAGAGTTACTTTGATAGAGT AAATAATAGGAGCTTAAACCCCCTTATTTCTAGGACTATGAGAATCGAAC CCATCCCTGAGAATCCAAAATTCTCCGTGCCACCTATCACACCCCATCCT AAAGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTG GTTATACCCTTCCCGTACTAATTAATCCCCTGGCCCAACCCGTCATCTAC TCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATT TTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTC TAACCAAAAAAATAAACCCTCGTTCCACAGAAGCTGCCATCAAGTATTTC CTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAA CAATATACTCTCCGGACAATGAACCATAACCAATACTACCAatcaatact catcattaataatcataatGGCTATAGCAATAAAACTAGGAATAGCCCCC TTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACATCCGG CCTGCTTCTTCTCACATGACAAAAACTAGCCCCCATCTCAATCATATACC AAATCTCTCCCTCACTAAACGTAAGCCTTCTCCTCACTCTCTCAATCTTA TCCATCATAGCAGGCAGTTGAGGTGGATTAAACCAAACCCAGCTACGCAA AATCTTAGCATACTCCTCAATTACCCACATAGGATGAATAATAGCAGTTC TACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATC CTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGAC CCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAA TTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTT TTGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATAGCCTCAT CATCCCCACcatcatagccaccatcaCCCTCCTTAACCTCTACTTCTACC TACGCCTAATCTACTCCACCTCAATCACACTACTCCCCATATCTAACAAC GTAAAAATAAAATGACAGTTTGAACATACAAAACCCACCCCATTCCTCCC CACACTCATCGCCCTTACCACGCTACTCCTACCTATCTCCCCTTTTATAC TAATAATCTTATAGAAATTTAGGTTAAATACAGACCAAGAGCCTTCAAAG CCCTCAGTAAGTTGCAATACTTAATTTCTGCAACAGCTAAGGACTGCAAA ACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAA GCCCTTACTAGACCAATGGGACTTAAACCCACAAACACTTAGTTAACAGC TAAGCACCCTAATCAACTGGCTTCAATCTACTTCTCCCGCCGCCGGGAAA AAAGGCGGGAGAAGCCCCGGCAGGTTTGAAGCTGCTTCTTCGAATTTGCA ATTCAATATGAAAATCACCTCGGAGCTGGTAAAAAGAGGCCTAACCCCTG TCTTTAGATTTACAGTCCAATGCTTCACTCAGCCATTTTACCTCACCCCC ACTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTG GAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCT CTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGG TAACGACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAA TCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTA GTTCCCCTAAtaaTCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAA CATAAGCTTCTGACTCTTACCTCCCTCTCTCCTACTCCTGCTCGCATCTG CTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA GCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTC CTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCA CAACAATTATCAATATAAAACCCCCTGCCATAACCCAATACCAAACGCCC CTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT CCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA AcaccaccttcttcgaccccgccggaggaggagACCCCATTCTATACCAA CACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACC AGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAG AACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTC CTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGT AGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCA CCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATG AAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCAC CGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCG TACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCA ATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCT ATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTA TCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGC CTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCAC ATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAA TattaataattttcatgatTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTC CTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCC CCCACCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAAA AAAGGAAGGAATCGAACCCCCCAAAGCTGGTTTCAAGCCAACCCCATGGC CTCCATGACTTTTTCAAAAAGGTATTAGAAAAACCATTTCATAACTTTGT CAAAGTTAAATTATAGGCTAAATCCTATATATCTTAATGGCACATGCAGC GCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCA CCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTC CTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACAT CTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCA TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC GAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCCACCAATGGTA CTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACA TACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTT GACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTAC ATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAA CAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCGCTACACGA CCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAG TTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAG GGCCCGTATTTACCCTATAGCACCCCCTCTaccccctctaGAGCCCACTG TAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAGATTAAGAGAACCAAC ACCTCTTTACAGTGAAATGCCCCAACTAAATACTACCGTATGGCCCACCA TAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAAATA TTAAACACAAACTACCACCTACCTCCCTCACCAAAGCCCATAAAAATAAA AAATTATAACAAACCCTGAGAACCAAAATGAACGAAAATCTGTTCGCTTC ATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTC TATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGA CTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATGAT AGCCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCT TAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCA TTTACACCAAccacccaactatctATAAACCTAGCCATGGCCATCCCCTT ATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCC TAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTA GTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGT ACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTG GAAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATC ATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTT AATCCAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCACGACA ACACATAATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC CATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTA GCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGCCTACT AACCAACACACTAACCATATACCAATGGTGGCGCGATGTAACACGAGAAA GCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATAC GGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTT CTGAGCCTTTTACCACTCCAGCCTAGCCCCTACCCCCCAACTAGGAGGGC ACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTC CTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCA CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA TTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTAC TTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTT TGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCC TCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACAT CACTTTGGCTTCGAAGCCGCCGCCTGATACTGGCATTTTGTAGATGTGGT TTGACTATTTCTGTATGTCTCCATCTATTGATGAGGGTCTTACTCTTTTA GTATAAATAGTACCGTTAACTTCCAATTAACTAGTTTTGACAACATTCAA AAAAGAGTAATAAACTTCGCCTTAATTTTAATAATCAACACCCTCCTAGC CTTACtactaataattattaCATTTTGACTACCACAACTCAACGGCTACA TAGAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCC CGCGTCCCTTTCTCCATAAAATTCTTCTTAGTAGCTATTACCTTCTTATT ATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCATGAGCCCTACAAA CAACTAACCTGCCACTAATAGTTATGTCATCCCTCTTATTaatcatcatC CTAGCCCTAAGTCTGGCCTATGAGTGACTACAAAAAGGATTAGACTGAGC CGAATTGGTATATAGTTTAAACAAAACGAATGATTTCGACTCATTAAATT ATGATAATCATATTTACCAAATGCCCCTCATTTACATAAATATTATACTA GCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCTCAT ATCCTCCCTACTATGCCTAGAAGGAATAATACTATCGCTGTTCATTATAG CTACTCTCATAACCCTCAACACCCACTCCCTCTTAGCCAATATTGTGCCT ATTGCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCT ACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGTACATAACCTAA ACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTACTACCAC TGACATGACTTTCCAAAAAGCACATAATTTGAATCAACACAACCACCCAC AGCCTAATTATTAGcatcatccccctactattttTTAACCAAATCAACAA CAACCTATTTAGCTGTTCCCCAACCTTTTCCTCCGACCCCCTAACAACCC CCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGGCAAGC CAACGCCACTTATCCAGCGAACCACTATCACGAAAAAAACTCTACCTCTC TATACTAAtctccctacaaatctccttaATTATAACATTCACAGCCACAG AACTAATCATATTTTATATCTTCTTCGAAACCACACTTATCCCCACCTTG GCTATCATCACCCGATGAGGCAACCAGCCAGAACGCCTGAACGCAGGCAC ATACTTCCTATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCATCGCAC TAATTTACACTCACAACACCCTAGGCTCACTAAACATTCTACTACTCACT CTCACTGCCCAAGAACTATCAAACTCCTGAGCCAACAACTTAATATGACT AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT TATGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAATAGTA CTTGCCGCAGTACTCTTAAAACTAGGCGGCTATGGTATAATACGCCTCAC ACTCATTCTCAACCCCCTGACAAAACACATAGCCTACCCCTTCCTTGTAC TATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACA GACCTAAAATCGCTCATTGCATACTCTTCAATCAGCCACATAGCCCTCGT AGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAGTCA TTCTCATAATCGCCCACGGACTCACATCCTCATTACTATTCTGCCTAGCA AACTCAAACTACGAACGCACTCACAGTCGCATCATAATCCTCTCTCAAGG ACTTCAAACTCTACTCCCACTAATAGCTTTTTGATGACTTCTAGCAAGCC TCGCTAACCTCGCCTTACCCCCCACTATTAACCTACTGGGAGAACTCTCT GTGCTAGTAACCACGTTCTCCTGATCAAATATCACTCTCCTACTTACAGG ACTCAACATACTAGTCACAGCCCTATACTCCCTCTACATATTTACCACAA CACAATGGGGCTCACTCACCCACCACATTAACAACATAAAACCCTCATTC ACACGagaaaacACCCTCATGTTCATACACCTATCCCCCATTCTCCTCCT ATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATAGTT TAACCAAAACATCAGATTGTGAATCTGACAACAGAGGCTTACGACCCCTT ATTTACCGAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAA CAACATGGCTTTCTCAACTTTTAAAGGATAACAGCTATCCATTGGTCTTA GGCCccaaaaatttTGGTGCAACTCCAAATAAAAGTAATAACCATGCACA CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC ACCCTCGTTAACCCTAACAAAAAAAACTCATACCCCCATTATGTAAAATC CATTGTCGCATCCACCTTTATTATCAGTCTCTTCCCCACAACAATATTCA TGTGCCTAGACCAAGAAGTTATTATCTCGAACTGACACTGAGCCACAACC CAAACAACCCAGCTCTCCCTAAGCTTCAAACTAGACTACTTCTCCATAAT ATTCATCCCTGTAGCATTGTTCGTTACATGGTCCATCATAGAATTCTCAC TGTGATATATAAACTCAGACCCAAACATTAATCAGTTCTTCAAATATCTA CTCATTTTCCTAATTACCATACTAATCTTAGTTACCGCTAACAACCTATT CCAACTGTTCATCGGCTGAGAGGGCGTAGGAATTATATCCTTCTTGCTCA TCAGTTGATGATACGCCCGAGCAGATGCCAACACAGCAGCCATTCAAGCA GTCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATG ATTTATCCTACACTCCAACTCATGAGACCCACAACAAATAGCCCTTCTAA ACGCTAATCCAAGCCTCACCCCACTACTAGGCCTcctcctagcagcagca gGCAAATCAGCCCAATTAGGTCTCCACCCCTGACTCCCCTCAGCCATAGA AGGCCCCACCCCAGTCTCAGCCCTACTCCACTCAAGCACTATAGTTGTAG CAGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCAGAAAATAGCCCA CTAATCCAAACTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGC AGCAGTCTGCGCCCTTACACAAAATGACATCAAAAAAATCGTAGCCTTCT CCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCATCAACCAA CCACACCTAGCATTCCTGCACATCTGTACCCACGCCTTCTTCAAAGCCAT ACTATTTATGTGCTCCGGGTCCATCATCCACAACCTTAACAATGAACAAG ATATTCGAAAAATAGGAGGACTACTCAAAACCATACCTCTCACTTCAACC TCCCTCACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTCACAGG TTTCTACTCCAAAGACCACATCATCGAAACCGCAAACATATCATACACAA ACGCCTGAGCCCTATCTATTACTCTCATCGCTACCTCCCTGACAAGCGCC TATAGCACTCGAATAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCC CACCCTTACTAACATTAACGAAAATAACCCCACCCTACTAAACCCCATTA AACGCCTGGCAGCCGGAAGCCTATTCGCAGGATTTCTCATTACTAACAAC ATTTCCCCCGCATCCCCCTtccaaacaacaatccccCTCTACCTAAAACT CACAGCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCA ACTACCTAACCAACAAACTTAAAATAAAATCCCCACTATGCACATTTTAT TTCTCCAACATACTCGGATTCTACCCTAGCATCACACACCGCACAATCCC CTATCTAGGCCTTCTTACGAGCCAAAACCTGCCCCTACTCCTCCTAGACC TAACCTGACTAGAAAAGCTATTACCTAAAACAATTTCACAGCACCAAATC TCCACCTCCATCATCACCTCAACCCAAAAAGGCATAATTAAACTTTACTT CCTCTCTTTcttcttcccactcatcctaaccctactcctaaTCACATAAC CTATTCCCCCGAGCAATCTCAATTACAATATATACACCAACAAACAATGT TCAACCAGTAACCACTACTAATCAACGCCCATAATCATACAAAGCCCCCG CACCAATAGGATCCTCCCGAATCAACCCTGACCCCTCTCCTTCATAAATT ATTCAGCTTCCTACACTATTAAAGTTTACCACAaccaccaccccATCATA CTCTTTCACCCACAGCACCAATCCTACCTCCATCGCTAACCCCACTAAAA CACTCACCAAGACCTCAACCCCTGACCCCCATGCCTCAGGATACTCCTCA ATAGCCATCGCTGTAGTATATCCAAAGACAACCATCATTCCCCCTAAATA AATtaaaaaaactattaaacCCATATAACCTCCCCCAAAATTCAGAATAA TAACACACCCGACCACACCGCTAACAATCAGTACTAAACCCCCATAAATA GGAGAAGGCTTAGAAGAAAACCCCACAAACCCCATTACTAAACCCACACT CAACAGAAACAAAGCATACATCATTATTCTCGCACGGACTACAACCACGA CCAATGATATGAAAAACCATCGTTGTATTTCAACTACAAGAACACCAATG ACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATT CATCGACCTCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCT CACTCCTTGGCGCCTGCCTGATCCTCCAAATCACCACAGGACTATTCCTA GCCATACACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCA CATCACTCGAGACGTAAATTATGGCTGAATCATCCGCTACCTTCACGCCA ATGGCGCCTCAATATTCTTTATCTGCCTCTTCCTACACATCGGGCGAGGC CTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTAT CCTCCTGCTTGCAACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGT GAGGCCAAATATCATTCTGAGGGGCCACAGTAATTACAAACTTACTATCC GCCATCCCATACATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTA CTCAGTAGACAGTCCCACCCTCACACGATTCTTTACCTTTCACTTCATct tacccttcaTTATTGCAGCCCTAGCAGCACTCCACCTCCTATTCTTGCAC GAAACGGGATCAAACAACCCCCTAGGAATCACCTCCCATTCCGATAAAAT CACCTTCCACCCTTACTACACAATCAAAGACGCCCTCGGCTTACTTCTct tccttctctccttaatGACATTAACACTATTCTCACCAGACCTCCTAGGC GACCCAGACAATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACAT CAAGCCCGAATGATATTTCCTATTCGCCTACACAATTCTCCGATCCGTCC CTAACAAACTAGGAGGCGTCCTTGCCCTATTACTATCCATCCTCATCCTA GCAATAATCCCCATCCTCCATATATCCAAACAACAAAGCATAATATTTCG CCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCAGACCTCCTCATTC TAACCTGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGA CAAGTAGCATCCGTACTATACTTCACAACAATCCTaatcctaatacCAAC TATCTCCCTAATTGAAAACAAAATACTCAAATGGGCCTGTCCTTGTAGTA TAAACTAATACACCAGTCTTGTAAACCGGAGACGAAAACCTTTTTCCAAG GACAAATCAGAGAAAAAGTCTTTAACTCCACCATTAGCACCCAAAGCTAA GATTCTAATTTAAACTATTCTCTGTTCTTTCATGGGGAAGCAGATTTGGG TACCACCCAAGTATTGACTCACCCATCAACAACCGCTATGTATTTCGTAC ATTACTGCCAGCCACCATGAATATTGTACGGTACCATAAATACTTGACCA CCTGTAGTACATAAAAACCCAACCCACATCAaaccccccccccccATGCT TACAAGCAAGTACAGCAATCAACCTTCAACTATCACACATCAACTGCAAC TCCAAAGCCACCCCTCACCCACTAGGATACCAACAAACCTACCCACCCTT AACAGTACATAGTACATAAAGTCAtttaccgtacatagcacatTACAGTC AAATCCCTTCTCGTCCCCATGGATGACCCCCCTCAGATAGGGGTCCCTTG ACCACCATCCTCCGTGAAATCAATATCCCGCACAAGAGTGCTACTCTCCT CGCTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGAC ATCTGGTTCCTACTTCAGGGCCATAAAGCCTAAATAGCCCACACGTTCCC CTTAAATAAGACATCACGATG >sp|Q8WZ42|TITIN_HUMAN MTTQAPTFTQPLQSVVVLEGSTATFEAHISGFPVPEVSWFRDGQVISTST LPGVQISFSDGRAKLTIPAVTKANSGRYSLKATNGSGQATSTAELLVKAE TAPPNFVQRLQSMTVRQGSQVRLQVRVTGIPTPVVKFYRDGAEIQSSLDF QISQEGDLYSLLIAEAYPEDSGTYSVNATNSVGRATSTAELLVQGEEEVP AKKTKTIVSTAQISESRQTRIEKKIEAHFDARSIATVEMVIDGAAGQQLP HKTPHRIPPKPKSRSxxxPSIAAKAQLARQQSPSPIxxxxxxxxxxxxxx PSPVRSVSPAAxxxxxxxxxxxxxxLMRKTQASTVATGPEVPPPWKQEGY VASSSEAEMRETTLTTSTQIRTEERWEGRYGVQExxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxDMARVREPVISAVEQTA QRTTTTAVHIQxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxISEEITxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxVPGAQEETTTQQxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxPREPGHLEESYAQQTTLEYGYKERISAAKVAEPP QRPASEPHVVPKAVKPRVIQAPSETHIKTTDQKGMHISSQIKKTTDLTTE RLVHVDKRPRTASPHFTVSKISVPKTEHGYEASIAGSAIATLQKELSATS SAQKITKSVKAPTVKPSETRVRAEPTPLPQFPFADTPDTYKSEAGVEVKK EVGVSITGTTVREERFEVLHGREAKVTETARVPAPVEIPVTPPTLVSGLK NVTVIEGESVTLECHISGYPSPTVTWYREDYQIESSIDFQITFQSGIARL MIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFEKETTAVTEKFT TEEKRFVESRDVVMTDTSLTEEQAGPGEPAAPYFITKPVVQKLVEGGSVV FGCQVGGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADD AGEYTIVVRNKHGETSASASLLEEADYELLMKSQQEMLYQTQVTAFVQEP KVGETAPGFVYSEYEKxxxxxQALIRKKMAKDTVVVRTYVEDQEFHISSF EERxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxSGFDLRIKNY RILEGMGVTFHCKMSGYPLPKIAWYKDGKRIKHGERYQMDFLQDGRASLR IPVVLPEDEGIYTAFASNIKGNAICSGKLYVEPAAPLGAPTYIPTLEPVS RIRSLSPxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxLEETDESQ LERLYKPVFVLKPVSFKCLEGQTARFDLKVVGRPMPETFWFHDGQQIVND YTHKVVIKEDGTQSLIIVPATPSDSGEWTVVAQNRAGRSSISVILTVEAV EHQVKPMFVEKLKNVNIKEGSQLEMKVRATGNPNPDIVWLKNSDIIVPHK YPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGRDTTRCKVNVEVE FAEPEPERKLIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQ QKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANR LRMINEFGYCSLDYGVAYSRDSGIITCRATNKYGTDHTSATLIVKDEKSL VEESQLPEGRKGLQRIEELERMAHEGALTGVTTDQKEKQKPDIVLYPEPV RVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDGIHYLDIV DCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRRAPEPRPEFH VHEPGKLQFEVQKVDRPVDTTETKEVVKLKRAERITHEKVPEESEELRSK FKRRTEEGYYEAITAVELKSRKKDESYEELLRKTxxxxxxxxxxxxxxxx xxxxxxxxITIPTFKPDKIELSPSMEAPKIFERIQSQTVGQGSDAHFRVR VVGKPDPECEWYKNGVKIERSDRIYWYWPEDNVCELVIRDVTAEDSASIM VKAINIAGETSSHAFLLVQAKQLITFTQELQDVVAKEKDTMATFECETSE PFVKVKWYKDGMEVHEGDKYRMHSDRKVHFLSILTIDTSDAEDYSCVLVE DENVKTTAKLIVEGAVVEFVKELQDIEVPESYSGELECIVSPENIEGKWY HNDVELKSNGKYTITSRRGRQNLTVKDVTKEDQGEYSFVIDGKKTTCKLK MKPRPIAILQGLSDQKVCEGDIVQLEVKVSLESVEGVWMKDGQEVQPSDR VHIVIDKQSHMLLIEDMTKEDAGNYSFTIPALGLSTSGRVSVYSVDVITP LKDVNVIEGTKAVLECKVSVPDVTSVKWYLNDEQIKPDDRVQAIVKGTKQ RLVINRTHASDEGPYKLIVGRVETNCNLSVEKIKIIRGLRDLTCTETQNV VFEVELSHSGIDVLWNFKDKEIKPSSKYKIEAHGKIYKLTVLNMMKDDEG KYTFYAGENITSGKLTVAGGAISKPLTDQTVAESQEAVFECEVANPDSKG EWLRDGKHLPLTNNIRSESDGHKRRLIIAATKLDDIGEYTYKVATSKTSA KLKVEAVKIKKTLKNLTVTETQDAVFTVELTHPNVKGVQWIKNGVVLESN EKYAISVKGTIYSLRIKNCAIVDESVYGFRLGRLGASARLHVETVKIIKK PKDVTALENATVAFEVSVSHDTVPVKWFHKNVEIKPSDKHRLVSERKVHK LMLQNISPSDAGEYTAVVGQLECKAKLFVETLHITKTMKNIEVPETKTAS FECEVSHFNVPSMWLKNGVEIEMSEKFKIVVQGKLHQLIIMNTSTEDSAE YTFVCGNDQVSATLTVTPIMITSMLKDINAEEKDTITFEVTVNYEGISYK WLKNGVEIKSTDKCQMRTKKLTHSLNIRNVHFGDAADYTFVAGKATSTAT LYVEARHIEFRKHIKDIKVLEKKRAMFECEVSEPDITVQWMKDDQELQIT DRIKIQKEKYVHRLLIPSTRMSDAGKYTVVAGGNVSTAKLFVEGRDVRIR SIKKEVQVIEKQRAVVEFEVNEDDVDAHWYKDGIEINFQVQERHKYVVER RIHRMFISETRQSDAGEYTFVAGRNRSSVTLYVNAPEPPQVLQELQPVTV QSGKPARFCAVISGRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIE AFPEDAAVYTCEAKNDYGVATTSASLSVEVPEVVSPDQEMPVYPPAIITP LQDTVTSEGQPARFQCRVSGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQ LEIAEAYPEDEGTYTFVASNAVGQVSSTANLSLEAPESILHERIEQEIEM EMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYSTKFDSEKEG TGPIFIKEVSNADISMGDVATLSVTVIGIPKPKIQWFFNGVLLTPSADYK FVFDGDDHSLIILFTKLEDEGEYTCMASNDYGKTICSAYLKINSKGEGHK DTETESAVAKSLEKLGGPCPPHFLKELKPIRCAQGLPAIFEYTVVGEPAP TVTWFKENKQLCTSVYYTIIHNPNGSGTFIVNDPQREDSGLYICKAENML GESTCAAELLVLLEDTDMTDTPCKAKSTPEAPEDFPQTPLKGPAVEALDS EQEIATFVKDTILKAALITEENQQLSYEHIAKANELSSQLPLGAQELQSI LEQDKLTPESTREFLCINGSIHFQPLKEPSPNLQLQIVQSQKTFSKEGIL MPEEPETQAVLSDTEKIFPSAMSIEQINSLTVEPLKTLLAEPEGNYPQSS IEPPMHSYLTSVAEEVLSPKEKTVSDTNREQRVTLQKQEAQSALILSQSL AEGHVESLQSPDVMISQVNYEPLVPSEHSCTEGGKILIESANPLENAGQD SAVRIEEGKSLRFPLALEEKQVLLKEEHSDNVVMPPDQIIESKREPVAIK KVQEVQGRDLLSKESLLSGIPEEQRLNLKIQICRALQAAVASEQPGLFSE WLRNIEKVEVEAVNITQEPRHIMCMYLVTSAKSVTEEVTIIIEDVDPQMx xxxxxxxxALxAIIYEEIDILTAEGPRIQQGAKTSLQEEMDSFSGSQKVE PITEPEVESKYLISTEEVSYFNVQSRVKYLDATPVTKGVASAxxxxxxxx xxxxxxxxxxxxxxxxxxxxVATVKIQEAEGGLIKEDGPMIHTPLVDTVS EEGDIVHLTTSITNAKEVNWYFENKLVPSDEKFKCLQDQNTYTLVIDKVN TEDHQGEYVCEALNDSGKTATSAKLTVVKRAAPVIKRKIEPLEVALGHLA KFTCEIQSAPNVRFQWFKAGREIYESDKCSIRSSKYISSLEILRTQVVDC GEYTCKASNEYGSVSCTATLTVTEAYPPTFLSRPKSLTTFVGKAAKFICT VTGTPVIETIWQKDGAALSPSPNWKISDAENKHILELSNLTIQDRGVYSC KASNKFGADICQAELIIIDKPHFIKELEPVQSAINKKVHLECQVDEDRKV TVTWSKDGQKLPPGKDYKICFEDKIATLEIPLAKLKDSGTYVCTASNEAG SSSCSATVTVREPPSFVKKVDPSYLMLPGESARLHCKLKGSPVIQVTWFK NNKELSESNTVRMYFVNSEAILDITDVKVEDSGSYSCEAVNDVGSDSCST EIVIKEPPSFIKTLEPADIVRGTNALLQCEVSGTGPFEISWFKDKKQIRS SKKYRLFSQKSLVCLEIFSFNSADVGEYECVVANEVGKCGCMATHLLKEP PTFVKKVDDLIALGGQTVTLQAAVRGSEPISVTWMKGQEVIREDGKIKMS FSNGVAVLIIPDVQISFGGKYTCLAENEAGSQTSVGELIVKEPAKIIERA ELIQVTAGDPATLEYTVAGTPELKPKWYKDGRPLVASKKYRISFKNNVAQ LKFYSAELHDSGQYTFEISNEVGSSSCETTFTVLDRDIAPFFTKPLRNVD SVVNGTCRLDCKIAGSLPMRVSWFKDGKEIAASDRYRIAFVEGTASLEII RVDMNDAGNFTCRATNSVGSKDSSGALIVQEPPSFVTKPGSKDVLPGSAV CLKSTFQGSTPLTIRWFKGNKELVSGGSCYITKEALESSLELYLVKTSDS GTYTCKVSNVAGGVECSANLFVKEPATFVEKLEPSQLLKKGDATQLACKV TGTPPIKITWFANDREIKESSKHRMSFVESTAVLRLTDVGIEDSGEYMCE AQNEAGSDHCSSIVIVKESPYFTKEFKPIEVLKEYDVMLLAEVAGTPPFE ITWFKDNTILRSGRKYKTFIQDHLVSLQILKFVAADAGEYQCRVTNEVGS SICSARVTLREPPSFIKKIESTSSLRGGTAAFQATLKGSLPITVTWLKDS DEITEDDNIRMTFENNVASLYLSGIEVKHDGKYVCQAKNDAGIQRCSALL SVKEPATITEEAVSIDVTQGDPATLQVKFSGTKEITAKWFKDGQELTLGS KYKISVTDTVSILKIISTEKKDSGEYTFEVQNDVGRSSCKARINVLDLII PPSFTKKLKKMDSIKGSFIDLECIVAGSHPISIQWFKDDQEISASEKYKF SFHDNTAFLEISQLEGTDSGTYTCSATNKAGHNQCSGHLTVKEPPYFVEK PQSQDVNPNTRVQLKALVGGTAPMTIKWFKDNKELHSGAARSVWKDDTST SLELFAAKATDSGTYICQLSNDVGTATSKATLFVKEPPQFIKKPSPVLVL RNGQSTTFECQITGTPKIRVSWYLDGNEITAIQKHGISFIDGLATFQISG ARVENSGTYVCEARNDAGTASCSIELKVKEPPTFIRELKPVEVVKYSDVE LECEVTGTPPFEVTWLKNNREIRSSKKYTLTDRVSVFNLHITKCDPSDTG EYQCIVSNEGGSCSCSTRVALKEPPSFIKKIENTTTVLKSSATFQSTVAG SPPISITWLKDDQILDEDDNVYISFVDSVATLQIRSVDNGHSGRYTCQAK NESGVERCYAFLLVQEPAQIVEKAKSVDVTEKDPMTLECVVAGTPELKVK WLKDGKQIVPSRYFSMSFENNVASFRIQSVMKQDSGQYTFKVENDFGSSS CDAYLRVLDQNIPPSFTKKLTKMDKVLGSSIHMECKVSGSLPISAQWFKD GKEISTSAKYRLVCHERSVSLEVNNLELEDTANYTCKVSNVAGDDACSGI LTVKEPPSFLVKPGRQQAIPDSTVEFKAILKGTPPFKIKWFKDDVELVSG PKCFIGLEGSTSFLNLYSVDASKTGQYTCHVTNDVGSDSCTTMLLVTEPP KFVKKLEASKIVKAGDSSRLECKIAGSPEIRVVWFRNEHELPASDKYRMT FIDSVAVIQMNNLSTEDSGDFICEAQNPAGSTSCSTKVIVKEPPVFSSFP PIVETLKNAEVSLECELSGTPPFEVVWYKDKRQLRSSKKYKIASKNFHTS IHILNVDTSDIGEYHCKAQNEVGSDTCVCTVKLKEPPRFVSKLNSLTVVA GEPAELQASIEGAQPIFVQWLKEKEEVIRESENIRITFVENVATLQFAKA EPANAGKYICQIKNDGGMEENMATLMVLEPAVIVEKAGPMTVTVGETCTL ECKVAGTPELSVEWYKDGKLLTSSQKHKFSFYNKISSLRILSVERQDAGT YTFQVQNNVGKSSCTAVVDVSDRAVPPSFTRRLKNTGGVLGASCILECKV AGSSPISVAWFHEKTKIVSGAKYQTTFSDNVCTLQLNSLDSSDMGNYTCV AANVAGSDECRAVLTVQEPPSFVKEPEPLEVLPGKNVTFTSVIRGTPPFK VNWFRGARELVKGDRCNIYFEDTVAELELFNIDISQSGEYTCVVSNNAGQ ASCTTRLFVKEPAAFLKRLSDHSVEPGKSIILESTYTGTLPISVTWKKDG FNITTSEKCNIxxxxxTCILEILNSTKRDAGQYSCEIENEAGRDVCGALV STLEPPYFVTELEPLEAAVGDSVSLQCQVAGTPEITVSWYKGDTKLRPTP EYRTYFTNNVATLVFNKVNINDSGEYTCKAENSIGTASSKTVFRIQERQL PPSFARQLKDIEQTVGLPVTLTCRLNGSAPIQVCWYRDGVLLRDDENLQT SFVDNVATLKILQTDLSHSGQYSCSASNPLGTASSSARLTAREPKKSPFF DIKPVSIDVIAGESADFECHVTGAQPMRITWSKDNKEIRPGGNYTITCVG NTPHLRILKVGKGDSGQYTCQATNDVGKDMCSAQLSVKEPPKFVKKLEAS KVAKQGESIQLECKISGSPEIKVSWFRNDSELHESWKYNMSFINSVALLT INEASAEDSGDYICEAHNGVGDASCSTALTVKAPPVFTQKPSPVGALKGS DVILQCEISGTPPFEVVWVKDRKQVRNSKKFKITSKHFDTSLHILNLEAS DVGEYHCKATNEVGSDTCSCSVKFKEPPRFVKKLSDTSTLIGDAVELRAI VEGFQPISVVWLKDRGEVIRESENTRISFIDNIATLQLGSPEASNSGKYI CQIKNDAGMRECSAVLTVLEPARIIEKPEPMTVTTGNPFALECVVTGTPE LSAKWFKDGRELSADSKHHITFINKVASLKIPCAEMSDKGLYSFEVKNSV GKSNCTVSVHVSDRIVPPSFIRKLKDVNAILGASVVLECRVSGSAPISVG WFQDGNEIVSGPKCQSSFSENVCTLNLSLLEPSDTGIYTCVAANVAGSDE CSAVLTVQEPPSFEQTPDSVEVLPGMSLTFTSVIRGTPPFKVKWFKGSRE LVPGESCNISLEDFVTELELFEVQPLESGDYSCLVTNDAGSASCTTHLFV KEPATFVKRLADFSVETGSPIVLEATYTGTPPISVSWIKDEYLISQSERC SITMTEKSTILEILESTIEDYAQYSCLIENEAGQDICEALVSVLEPPYFI EPLEHVEAVIGEPATLQCKVDGTPEIRISWYKEHTKLRSAPAYKMQFKNN VASLVINKVDHSDVGEYSCKADNSVGAVASSAVLVIKERKLPPFFARKLK DVHETLGFPVAFECRINGSEPLQVSWYKDGVLLKDDANLQTSFVHNVATL QILQTDQSHIGQYNCSASNPLGTASSSAKLILSEHEVPPFFDLKPVSVDL ALGESGTFKCHVTGTAPIKITWAKDNREIRPGGNYKMTLVENTATLTVLK VGKGDAGQYTCYASNIAGKDSCSAHLGVQEPPRFIKKLEPSRIVKQDEFT RYECKIGGSPEIKVLWYKDETEIQESSKFRMSFVDSVAVLEMHNLSVEDS GDYTCEAHNAAGSASSSTSLKVKEPPIFRKKPHPIETLKGADVHLECELQ GTPPFHVSWYKDKRELRSGKKYKIMSENFLTSIHILNVDAADIGEYQCKA TNDVGSDTCVGSIALKAPPRFVKKLSDISTVVGKEVQLQTTIEGAEPISV VWFKDKGEIVRESDNIWISYSENIATLQFSRVEPANAGKYTCQIKNDAGM QECFATLSVLEPATIVEKPESIKVTTGDTCTLECTVAGTPELSTKWFKDG KELTSDNKYKISFFNKVSGLKIINVAPSDSGVYSFEVQNPVGKDSCTASL QVSDRTVPPSFTRKLKETNGLSGSSVVMECKVYGSPPISVSWFHEGNEIS SGRKYQTTLTDNTCALTVNMLEESDSGDYTCIATNMAGSDECSAPLTVRE PPSFVQKPDPMDVLTGTNVTFTSIVKGTPPFSVSWFKGSSELVPGDRCNV SLEDSVAELELFDVDTSQSGEYTCIVSNEAGKASCTTHLYIKAPAKFVKR LNDYSIEKGKPLILEGTFTGTPPISVTWKKNGINVTPSQRCNITTTEKSA ILEIPSSTVEDAGQYNCYIENASGKDSCSAQILILEPPYFVKQLEPVKVS VGDSASLQCQLAGTPEIGVSWYKGDTKLRPTTTYKMHFRNNVATLVFNQV DINDSGEYICKAENSVGEVSASTFLTVQEQKLPPSFSRQLRDVQETVGLP VVFDCAISGSEPISVSWYKDGKPLKDSPNVQTSFLDNTATLNIFKTDRSL AGQYSCTATNPIGSASSSARLILTEGKNPPFFDIRLAPVDAVVGESADFE CHVTGTQPIKVSWAKDSREIRSGGKYQISYLENSAHLTVLKVDKGDSGQY TCYAVNEVGKDSCTAQLNIKERLIPPSFTKRLSETVEETEGNSFKLEGRV AGSQPITVAWYKNNIEIQPTSNCEITFKNNTLVLQVRKAGMNDAGLYTCK VSNDAGSALCTSSIVIKEPKKPPVFDQHLTPVTVSEGEYVQLSCHVQGSE PIRIQWLKAGREIKPSDRCSFSFASGTAVLELRDVAKADSGDYVCKASNV AGSDTTKSKVTIKDKPAVAPATKKAAVDGRLFFVSEPQSIRVVEKTTATF IAKVGGDPIPNVKWTKGKWRQLNQGGRVFIHQKGDEAKLEIRDTTKTDSG LYRCVAFNEHGEIESNVNLQVDERKKQEKIEGDLRAMLKKTPILKKGAGE EEEIDIMELLKNVDPKEYEKYARMYGITDFRGLLQAFELLKQSQEEETHR LEIxxxxxxxxxxxxxxxxxSFIQQRLSQTEPVTLIKDIENQTVLKDNDA VFEIDIKINYPEIKLSWYKGTEKLEPSDKFEISIDGDRHTLRVKNCQLKD QGNYRLVCGPHIASAKLTVIEPAWERHLQDVTLKEGQTCTMTCQFSVPNV KSEWFRNGRILKPQGRHKTEVEHKVHKLTIADVRAEDQGQYTCKYEDLET SAELRIEAEPIQFTKRIQNIVVSEHQSATFECEVSFDDAIVTWYKGPTEL TESQKYNFRNDGRCHYMTIHNVTPDDEGVYSVIARLEPRGEARSTAELYL TTKEIKLELKPPDIPDSxxxxxxxxxxxxxxxxxxxxxxxxxPLLLPTxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxPAK APEIIDVSSKAEEVKIMTITRKKEVQKxxxxxxxxxxxxxxxxxxFIESx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxPVIEKIEKTSRRMEEEKVQVTKVPEVSKKIVPQKPxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxAxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xSFAVPQRVEVTRHEVSAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx EYEVMEEPxxxxxxxxxxxxxxxxxxxxxxxxxxxEKKIVLKPKIPAKxx xxxxxxxxxxxxxxxxxxKVPAPVPxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx EEKERAYTLEEEAVSVQRExxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxITEPEKPIxxxxxxxxxxxxxxxxxxxxxLKxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxSITKREKEQVxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxEVAxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxKxxxxxxxxxxxxxxxxxxxxxxxxxxxxVxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxKREPVPVPVALPQEEEVLxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxKVVIPxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxExxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxEKVxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxRTVLEEKVSVAFRQEVVVKERLELEVVEAE VEEIPxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxEKPKAPPKGPEISEKIIPPKKPPxxxxxxxxxxxxxxxVP xKxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxAxxxxxxxxxx xxxxxxxxxxxxxxxxxxxPERAESPPPEVYxxxxxxxxxxxxxxxxxxP VPVxxxxxxxxxxxxxxxxxxxxxxxxxxPVIxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxVELTPL KVPGGEKKVRKLLPERKPEPKEEVVLxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGKKAEAKAPKEEAAKPKGPI KGVPKKTPSPIEAERRKLRPGSGGEKPPDEAPFTYQLKAVPLKFVKEIKD IILTESEFVGSSAIFECLVSPSTAITTWMKDGSNIRESPKHRFIADGKDR KLHIIDVQLSDAGEYTCVLRLGNKEKTSTAKLVVEELPVRFVKTLEEEVT VVKGQPLYLSCELNKERDVVWRKDGKIVVEKPGRIVPGVIGLMRALTIND ADDTDAGTYTVTVENANNLECSSCVKVVEVIRDWLVKPIRDQHVKPKGTA IFACDIAKDTPNIKWFKGYDEIPAEPNDKTEILRDGNHLYLKIKNAMPED IAEYAVEIEGKRYPAKLTLGEREVELLKPIEDVTIYEKESASFDAEISEA DIPGQWKLKGELLRPSPTCEIKAEGGKRFLTLRKVKLDQAGEVLYQALNA ITTAILTVKEIELDFAVPLKDVTVPERRQARFECVLTREANVIWSKGPDI IKSSDKFDIIADGKKHILVINDSQFDDEGVYTAEVEGKKTSARLFVTGIR LKFMSPLEDQTVKEGETATFVCELSHEKMHVVWFKNDAKLHTSRTVLISS EGKTHKLEMKEVTLDDISQIKAQVKELSSTAQLKVLEADPYFTVKLHDKT AVEKDEITLKCEVSKDVPVKWFKDGEEIVPSPKYSIKADGLRRILKIKKA DLKDKGEYVCDCGTDKTKANVTVEARLIKVEKPLYGVEVFVGETAHFEIE LSEPDVHGQWKLKGQPLTASPDCEIIEDGKKHILILHNCQLGMTGEVSFQ AANAKSAANLKVKELPLIFITPLSDVKVFEKDEAKFECEVSREPKTFRWL KGTQEITGDDRFELIKDGTKHSMVIKSAAFEDEAKYMFEAEDKHTSGKLI IEGIRLKFLTPLKDVTAKEKESAVFTVELSHDNIRVKWFKNDQRLHTTRS VSMQDEGKTHSITFKDLSIDDTSQIRVEAMGMSSEAKLTVLEGDPYFTGK LQDYTGVEKDEVILQCEISKADAPVKWFKDGKEIKPSKNAVIKADGKKRM LILKKALKSDIGQYTCDCGTDKTSGKLDIEDREIKLVRPLHSVEVMETET ARFETEISEDDIHANWKLKGEALLQTPDCEIKEEGKIHSLVLHNCRLDQT GGVDFQAANVKSSAHLRVKPRVIGLLRPLKDVTVTAGETATFDCELSYED IPVEWYLKGKKLEPSDKVVPRSEGKVHTLTLRDVKLEDAGEVQLTAKDFK THANLFVKEPPVEFTKPLEDQTVEEGATAVLECEVSRENAKVKWFKNGTE ILKSKKYEIVADGRVRKLVIHDCTPEDIKTYTCDAKDFKTSCNLNVVPPH VEFLRPLTDLQVREKEMARFECELSRENAKVKWFKDGAEIKKGKKYDIIS KGAVRILVINKCLLDDEAEYSCEVRTARTSGMLTVLEEEAVFTKNLANIE VSETDTIKLVCEVSKPGAEVIWYKGxxxxxxxxxxxxxxxxxxxxxVIQN AHLEDAGNYNCRLPSSRTDGKVKVHELAAEFISKPQNLEILEGEKAEFVC SISKESFPVQWKRDDKTLESGDKYDVIADGKKRVLVVKDATLQDMGTYVV MVGAARAAAHLTVIEKLRIVVPLKDTRVKEQQEVVFNCEVNTEGAKAKWF RNEEAIFDSSKYIILQKDLVYTLRIRDAHLDDQANYNVSLTNHRGENVKS AANLIVEEEDLRIVEPLKDIETMEKKSVTFWCKVNRLNVTLKWTKNGEEV PFDNRVSYRVDKYKHMLTIKDCGFPDEGEYIVTAGQDKSVAELLIIEAPT EFVEHLEDQTVTEFDDAVFSCQLSREKANVKWYRNGREIKEGKKYKFEKD GSIHRLIIKDCRLDDECEYACGVEDRKSRARLFVEEIPVEIIRPPQDILE APGADVVFLAELNKDKVEVQWLRNNMVVVQGDKHQMMSEGKIHRLQICDI KPRDQGEYRFIAKDKEARAKLELAAAPKIKTADQDLVVDVGKPLTMVVPY DAYPKAEAEWFKENEPLSTKTIDTTAEQTSFRILEAKKGDKGRYKIVLQN KHGKAEGFINLKVIDVPGPVRNLEVTETFDGEVSLAWEEPLTDGGSKIIG YVVERRDIKRKTWVLATDRAESCEFTVTGLQKGGVEYLFRVSARNRVGTG EPVETDNxxEARSKYDVPGPPLNVTITDVNRFGVSLTWEPPEYDGGAEIT NYVIELRDKTSIRWDTAMTVRAEDLSATVTDVVEGQEYSFRVRAQNRIGV GKPSAATPFVKVADPIERPSPPVNLTSSDQTQSSVQLKWEPPLKDGGSPI LGYIIERCEEGKDNWIRCNMKLVPELTYKVTGLEKGNKYLYRVSAENKAG VSDPSEILGPLTADDAFVEPTMDLSAFKDGLEVIVPNPITILVPSTGYPR PTATWCFGDKVLETGDRVKMKTLSAYAELVISPSERSDKGIYTLKLENRV KTISGEIDVNVIARPSAPKELKFGDITKDSVHLTWEPPDDDGGSPLTGYV VEKREVSRKTWTKVMDFVTDLEFTVPDLVQGKEYLFKVCARNKCGPGEPA YVDEPVNMSTPATVPDPPENVKWRDRTANSIFLTWDPPKNDGGSRIKGYI VERCPRGSDKWVACGEPVAETKMEVTGLEEGKWYAYRVKALNRQGASKPS RPTEEIQAVDTQEAPEIFLDVKLLAGLTVKAGTKIELPATVTGKPEPKIT WTKADMILKQDKRITIENVPKKSTVTIVDSKRSDTGTYIIEAVNVCGRAT AVVEVNVLDKPGPPAAFDITDVTNESCLLTWNPPRDDGGSKITNYVVERR ATDSEVWHKLSSTVKDTNFKATKLIPNKEYIFRVAAENMYGVGEPVQASP ITAKYQFDPPGPPTRLEPSDITKDAVTLTWCEPDDDGGSPITGYWVERLD PDTDKWVRCNKMPVKDTTYRVKGLTNKKKYRFRVLAENLAGPGKPSKSTE PILIKDPIDPPWPPGKPTVKDVGKTSVRLNWTKPEHDGGAKIESYVIEML KTGTDEWVRVAEGVPTTQHLLPGLMEGQEYSFRVRAVNKAGExxxxxxxx xVLCREKLYPPSPPRWLEVINITKNTADLKWTVPEKDGGSPITNYIVEKR DVRRKGWQTVDTTVKDTKCTVTPLTEGSLYVFRVAAENAIGQSDYTEIED SVLAKDTFTTPGPPYALAVVDVTKRHVDLKWEPPKNDGGRPIQRYVIEKK ERLGTRWVKAGKTAGPDCNFRVTDVIEGTEVQFQVRAENEAGVGHPSEPx xxxxxxxxxxxxxxxLDLHVTDAGRKHIAIAWKPPEKNGGSPIIGYHVEM CPVGTEKWMRVNSRPIKDLKFKVEEGVVPDKEYVLRVRAVNAIGVSEPSE ISENVVAKDPDCKPTIDLETHDIIVIEGEKLSIPVPFRAVPVPTVSWHKD GKEVKASDRLTMKNDHISAHLEVPKSVRADAGIYTITLENKLGSATASIN VKVIGLPGPCKDIKASDITKSSCKLTWEPPEFDGGTPILHYVLERREAGR RTYIPVMSGENKLSWTVKDLIPNGEYFFRVKAVNKVGGGEYIELKNPVIA QDPKQPPDPPVDVEVHNPTAEAMTITWKPPLYDGGSKIMGYIIEKIAKGE ERWKRCNEHLVPILTYTAKGLEEGKEYQFRVRAENAAGISEPSRATPPTK AVDPIDAPKVILRTSLEVKRGDEIALDASISGSPYPTITWIKDENVIVPE EIKKRAAPLVRRRKGEVQEEEPFVLPLTQRLSIDNSKKGESQLRVRDSLR PDHGLYMIKVENDHGIAKAPCTVSVLDTPGPPINFVFEDIRKTSVLCKWE PPLDDGGSEIINYTLEKKDKTKPDSEWIVVTSTLRHCKYSVTKLIEGKEY LFRVRAENRFGPGPPCVSKPLVAKDPFGPPDAPDKPIVEDVTSNSMLVKW NEPKDNGSPILGYWLEKREVNSTHWSRVNKSLLNALKANVDGLLEGLTYV FRVCAENAAGPGKFSPPSDPKTAHDPISxxxxxxxxVTDTSSTTIELEWE PPAFNGGGEIVGYFVDKQLVGTNEWSRCTEKMIKVRQYTVKEIREGADYK LRVSAVNAAGEGPPGETQPVTVAEPQEPPAVELDVSVKGGIQIMAGKTLR IPAVVTGRPVPTKVWTKEEGELDKDRVVIDNVGTKSELIIKDALRKDHGR YVITATNSCGSKFAAARVEVFDVPGPVLDLKPVVTNRKMCLLNWSDPEDD GGSEITGFIIERKDAKMHTWRQPIETERSKCDITGLLEGQEYKFRVIAKN KFGCGPPVEIGPILAVDPLGPPTSPERLTYTERTKSTITLDWKEPRSNGG SPIQGYIIEKRRHDKPDFERVNKRLCPTTSFLVENLDEHQMYEFRVKAVN EIGESEPSLPLNVVIQDDEVPPTIKLRLSVRGDTIKVKAGEPVHIPADVT GLPMPKIEWSKNETVIEKPTDALQITKEEVSRSEAKTELSIPKAVREDKG TYTVTASNRLGSVFRNVHVEVYDRPSPPRNLAVTDIKAESCYLTWDAPLD NGGSEITHYVIDKRDASRKKAEWEEVTNTAVEKRYGIWKLIPNGQYEFRV RAVNKYGISDECKSDKVVIQDPYRLPGPPGKPKVLARTKGSMLVSWTPPL DNGGSPITGYWLEKREEGSPYWSRVSRAPITKVGLKGVEFNVPRLLEGVK YQFRAMAINAAGIGPPSEPSDPEVAGDPxxxxxxxxxxxxxDKTKSSISL GWKPPAKDGGSPIKGYIVEMQEEGTTDWKRVNEPDKLITTCECVVPNLKE LRKYRFRVKAVNEAGESEPSDTTGEIPATDIQEEPEVFIDIGAQDCLVCK AGSQIRIPAVIKGRPTPKSSWEFDGKAKKAMKDGVHDIPEDAQLETAENS SVIIIPECKRSHTGKYSITAKNKAGQKTANCRVKVMDVPGPPKDLKVSDI TRGSCRLSWKMPDDDGGDRIKGYVIEKRTIDGKAWTKVNPDCGSTTFVVP DLLSEQQYFFRVRAENRFGIGPPVETIQRTTARDPIYPPDPPIKLKIGLI TKNTVHLSWKPPKNDGGSPVTHYIVECLAWDPTGTKKEAWRQCNKRDVEE LQFTVEDLVEGGEYEFRVKAVNAAGVSKPSATVGPCDCQRPDMPPSIDLK EFMEVEEGTNVNIVAKIKGVPFPTLTWFKAPPKKPDNKEPVLYDTHVNKL VVDDTCTLVIPQSRRSDTGLYTITAVNNLGTASKEMRLNVLGRPGPPVGP IKFESVSADQMTLSWFPPKDDGGSKITNYVIEKREANRKTWVHVSSEPKE CTYTIPKLLEGHEYVFRIMAQNKYGIGEPLDSEPETARNLFSVPGAPDKP TVSSVTRNSMTVNWEEPEYDGGSPVTGYWLEMKDTTSKRWKRVNRDPIKA MTLGVSYKVTGLIEGSDYQFRVYAINAAGVGPASLPSDPATARDPIAPPG PPFPKVTDWTKSSADLEWSPPLKDGGSKVTGYIVEYKEEGKEEWEKxKDK EVRGTKLVVTGLKEGAFYKFRVSAVNIAGIGEPGEVTDVIEMKDRLVSPD LQLDASVRDRIVVHAGGVIRIIAYVSGKPPPTVTWNMNERTLPQEATIET TAISSSMVIKNCQRSHQGVYSLLAKNEAGERKKTIIVDVLDVPGPVGTPF LAHNLTNESCKLTWFSPEDDGGSPITNYVIEKRESDRRAWTPVTYTVTRQ NATVQGLIQGKAYFFRIAAENSIGMGPFVETSEALVIREPITVPERPEDL EVKEVTKNTVTLTWNPPKYDGGSEIINYVLESRLIGTEKFHKVTNDNLLS RKYTVKGLKEGDTYEYRVSAVNIVGQGKPSFCTKPITCKDELAPPTLHLD FRDKLTIRVGEAFALTGRYSGKPKPKVSWFKDEADVLEDDRTHIKTTPAT LALEKIKAKRSDSGKYCVVVENSTGSRKGFCQVNVVDRPGPPVGPVSFDE VTKDYMVISWKPPLDDGGSKITNYIIEKKEVGKDVWMPVTSASAKTTCKV SKLLEGKDYIFRIHAENLYGISDPLVSDSMKAKDRFRVPDAPDQPIVTEV TKDSALVTWNKPHDGGKPITNYILEKRETMSKRWARVTKDPIHPYTKFRV PDLLEGCQYEFRVSAENEIGIGDPSPPSKPVFAKDPIAxxxxxxxPEAID TTCNSVDLTWQPPRHDGGSKILGYIVEYQKVGDEEWRRANHTPESCPETK YKVTGLRDGQTYKFRVLAVNAAGESDPAHVPEPVLVKDRLEPPELILDAN MAREQHIKVGDTLRLSAIIKGVPFPKVTWKKEDRDAPTKARIDVTPVGSK LEIRNAAHEDGGIYSLTVENPAGSKTVSVKVLVLDKPGPPRDLEVSEIRK DSCYLTWKEPLDDGGSVITNYVVERRDVASAQWSPLSATSKKKSHFAKHL NEGNQYLFRVAAENQYGRGPFVETPKPIKALDPLHPPGPPKDLHHVDVDK TEVSLVWNKPDRDGGSPITGYLVEYQEEGTQDWIKFKTVTNLECVVTGLQ QGKTYRFRVKAENIVGLGLPDTTIPIECQEKLVPPSVELDVKLIEGLVVK AGTTVRFPAIIRGVPVPTAKWTTDGSEIKTDEHYTVETDNFSSVLTIKNC LRRDTGEYQITVSNAAGSKTVAVHxxxLDVPGPPTGPINILDVTPEHMTI SWQPPKDDGGSPVINYIVEKQDTRKDTWGVVSSGSSKTKLKIPHLQKGCE YVFRVRAENKIGVGPPLDSTPTVAKHKFSPPSPPGKPVVTDITENAATVS WTLPKSDGGSPITGYYMERREVTGKWVRVNKTPIADLKFRVTGLYEGNTY EFRVFAENLAGLSKPSPSSDPIKACRPIKPPGPPINPKLKDKSRETADLV WTKPLSDGGSPILGYVVECQKPGTAQWNRINKDELIRQCAFRVPGLIEGN EYRFRIKAANIVGEGEPRELAESVIAKDILHPPEVELDVTCRDVITVRVG QTIRILARVKGRPEPDITWTKEGKVLVREKRVDLIQDLPRVELQIKEAVR ADHGKYIISAKNSSGHAQGSAIVNVLDRPGPCQNLKVTNVTKENCTISWE NPLDNGGSEITNFIVEYRKPNQKGWSIVASDVTKRLIKANLLANNEYYFR VCAENKVGVGPTIETKTPILAINPIDRPGEPENLHIADKGKTFVYLKWRR PDYDGGSPNLSYHVERRLKGSDDWERVHKGSIKETHYMVDRCVENQIYEF RVQTKNEGGESDWVKTEEVVVKEDLQKPVLDLKLSGVLTVKAGDTIRLEA GVRGKPFPEVAWTKDKDATDLTRSPRVKIDTRADSSKFSLTKAKRSDGGK YVVTATNTAGSFVAYATVNVLDKPGPVRNLKIVDVSSDRCTVCWDPPEDD GGCEIQNYILEKCETKRMVWSTYSATVLTPGTTVTRLIEGNEYIFRVRAE NKIGTGPPTESKPVIAKTKYDKPGRPDPPEVTKVSKEEMTVVWNPPEYDG GKSITGYFLEKKEKHSTRWVPVNKSAIPERRMKVQNLLPDHEYQFRVKAE NEIGIGEPSLPSRPVVAKDPIEPPGPPTNFRVVDTTKHSITLGWGKPVYD GGAPIIGYVVEMRPKIADASPDEGWKRCNAAAQLVRKEFTVTSLDENQEY EFRVCAQNQVGIGRPAELKEAIKPKEILEPPEIDLDASMRKLVIVRAGCP IRLFAIVRGRPAPKVTWRKVGIDNVVRKGQVDLVDTMAFLVIPNSTRDDS GKYSLTLVNPAGEKAVFVNVRVLDTPGPVSDLKVSDVTKTSCHVSWAPPE NDGGSQVTHYIVEKREADRKTWSTVTPEVKKTSFHVTNLVPGNEYYFRVT AVNEYGPGVPTDVPKPVLASDPLSEPDPPRKLEVTEMTKNSATLAWLPPL RDGGAKIDGYITSYREEEQPADRWTEYSVVKDLSLVVTGLKEGKKYKFRV AARNAVGVSLPREAEGVYEAKEQLLPPKILMPEQITIKAGKKLRIEAHVY GKPHPTCKWKKGEDEVVTSSHLAVHKADSSSILIIKDVTRKDSGYYSLTA ENSSGTDTQKIKVVVMDAPGPPQPPFDISDIDADACSLSWHIPLEDGGSN ITNYIVEKCDVSRGDWVTALASVTKTSCRVGKLIPGQEYIFRVRAENRFG ISEPLTSPKMVAQFPFGVPSEPKNARVTKVNKDCIFVAWDRPDSDGGSPI IGYLIERKERNSLLWVKANDTLVRSTEYPCAGLVEGLEYSFRIYALNKAG SSPPSKPTEYVTARMPVDPPGKPEVIDVTKSTVSLIWARPKHDGGSKIIG YFVEACKLPGDKWVRCNTAPHQIPQEEYTATGLEEKAQYQFRAIARTAVN ISPPSEPSDPVTILAENVPPRIDLSVAMKSLLTVKAGTNVCLDATVFGKP MPTVSWKKDGTLLKPAEGIKMAMQRNLCTLELFSVNRKDSGDYTITAENS SGSKSATIKLKVLDKPGPPASVKINKMYSDRAMLSWEPPLEDGGSEITNY IVDKRETSRPNWAQVSATVPITSCSVEKLIEGHEYQFRICAENKYGVGDP VFTEPAIAKNPYDPPGRCDPPVISNITKDHMTVSWKPPADDGGSPITGYL LEKRETQAVNWTKVNRKPIIERTLKATGLQEGTEYEFRVTAINKAGPGKx xxxxxxxxxxxxxxxxxxxxxxKVYDTTRSSVSLSWGKPAYDGGSPIIGY LVEVKRADSDNWVRCNLPQNLQKTRFEVTGLMEDTQYQFRVYAVNKIGYS DPSDVPDKHYPKDILIPPEGELDADLRKTLILRAGVTMRLYVPVKGRPPP KITWSKPNVNLRDRIGLDIKSTDFDTFLRCENVNKYDAGKYILTLENSCG KKEYTIVVKVLDTPGPPVNVTVKEISKDSAYVTWEPPIIDGGSPIINYVV QKRDAERKSWSTVTTECSKTSFRVANLEEGKSYFFRVFAENEYGIGDPGE TRDAVKASQTPGPVVDLKVRSVSKSSCSIGWKKPHSDGGSRIIGYVVDFL TEENKWQRVMKSLSLQYSAKDLTEGKEYTFRVSAENENGEGTPSEITVVA RDDVVAPDLDLKGLPDLCYLAKENSNFRLKIPIKGKPAPSVSWKKGEDPL ATDTRVSVESSAVNTTLIVYDCQKSDAGKYTITLKNVAGTKEGTISIKVV GKPGIPTGPIKFDEVTAEAMTLKWAPPKDDGGSEITNYILEKRDSVNNKW VTCASAVQKTTFRVTRLHEGMEYTFRVSAENKYGVGEGLKSEPIVARHPF DVPDAPPPPNIVDVRHDSVSLTWTDPKKTGGSPITGYHLEFKERNSLLWK RANKTPIRMRDFKVTGLTEGLEYEFRVMAINLAGVGKPSLPSEPVVALDP IDPPGKPEVINITRNSVTLIWTEPKYDGGHKLTGYIVEKRDLPSKSWMKA NHVNVPECAFTVTDLVEGGKYEFRIRAKNTAGAISAPSESTETIICKDEY EAPTIVLDPTIKDGLxxxxxDTIVLNAISILGKPLPKSSWSKAGKDIRPS DITQITSTPTSSMLTIKYATRKDAGEYTITATNPFGTKVEHVKVTVLDVP GPPGPVEISNVSAEKATLTWTPPLEDGGSPIKSYILEKRETSRLLWTVVS EDIQSCRHVATKLIQGNEYIFRVSAVNHYGKGEPVQSEPVKMVDRFGPPx xxxxxxxxxxxxxxATVSWKRPVDDGGSEITGYHVERREKKSLRWVRAIK TPVSDLRCKVTGLQEGSTYEFRVSAENRAGIGPPSEASDSVLMKDAAYPP GPPSNPHVTDTTKKSASLAWGKPHYDGGLEITGYVVEHQKVGDEAWIKDT TGTALRITQFVVPDLQTKEKYNFRISAINDAGVGEPAVIPDVEIVEREMA PDFELDAELRRTLVVRAGLSIRIFVPIKGRPAPEVTWTKDNINLKNRANI ENTESFTLLIIPECNRYDTGKFVMTIENPAGKKSGFVNVRVLDTPGPVLN LRPTDITKDSVTLHWDLPLIDGGSRITNYIVEKREATRKSYSTATTKCHK CTYKVTGLSEGCEYFFRVMAENEYGIGEPTETTEPVKASEAPSPPDSLNI MDITKSTVSLAWPKPKHDGGSKITGYVIEAQRKGSDQWTHITTVKGLECV VRNLTEGEEYTFQVMAVNSAGRSAPRESRPVIVKEQTMLPELDLRGIYQK LVIAKAGDNIKVEIPVLGRPKPTVTWKKGDQILKQTQRVNFETTATSTIL NINECVRSDSGPYPLTARNIVGEVGDVITIQVHDIPGPPTGPIKFDEVSS DFVTFSWDPPENDGGVPISNYVVEMRQTDSTTWVELATTVIRTTYKATRL TTGLEYQFRVKAQNRYGVGPGITSACIVANYPFKVPGPPGTPQVTAVTKD SMTISWHEPLSDGGSPILGYHVERKERNGILWQTVSKALVPGNIFKSSGL TDGIAYEFRVIAENMAGKSKxxxxxxxxxxxxxxxxxxxxVPLNITRHTV TLKWAKPEYTGGFKITSYIVEKRDLPNGRWLKANFSNILENEFTVSGLTE DAAYEFRVIAKNAAGAISPPSEPSDAITCRDDVEAPKIKVDVKFKDTVIL KAGEAFRLEADVSGRPPPTMEWSKDGKELEGTAKLEIKIADFSTNLVNKD STRRDSGAYTLTATNPGGFAKHIFNVKVLDRPGPPEGPLAVTEVTSEKCV LSWFPPLDDGGAKIDHYIVQKRETSRLAWTNVASEVQVTKLKVTKLLKGN EYIFRVMAVNKYGVGEPLESEPxxxxxxxxxxxxxxxxxxxxITKDSMVV CWGHPDSDGGSEIINYIVERRDKAGQRWIKCNKKTLTDLRYKVSGLTEGH EYEFRIMAENAAGISAPSPTSPFYKACDTVFKPGPPGNPRVLDTSRSSIS IAWNKPIYDGGSEITGYMVEIALPEEDEWQIVTPPAGLKATSYTITGLTE NQEYKIRIYAMNSEGLGEPALVPGTPKAEDRMLPPEIELDADLRKVVTIR ACCTLRLFVPIKGRPAPEVKWARDHGESLDKASIESTSSYTLLIVGNVNR FDSGKYILTVENSSGSKSAFVNVRVLDTPGPPQDLKVKEVTKTSVTLTWD PPLLDGGSKIKNYIVEKRESTRKAYSTVATNCHKTSWKVDQLQEGCSYYF RVLAENEYGIGLPAETAESVKASERPLPPGKITLMDVTRNSVSLSWEKPE HDGGSRILGYIVEMQTKGSDKWATCATVKVTEATITGLIQGEEYSFRVSA QNEKGISDPRQLSVPVIAKDLVIPPAFKLLFNTFTVLAGEDLKVDVPFIG RPTPAVTWHKDNVPLKQTTRVNAESTENNSLLTIKDACREDVGHYVVKLT NSAGEAIETLNVIVLDKPGPPTGPVKMDEVTADSITLSWGPPKYDGGSSI NNYIVEKRDTSTTTWQIVSATVARTTIKACRLKTGCEYQFRIAAENRYGK STYLNSEPTVAQYPFKVPGPPGTPVVTLSSRDSMEVQWNEPISDGGSRVI GYHLERKERNSILWVKLNKTPIPQTKFKTTGLEEGVEYEFRVSAENIVGI GKPSKVSECYVARDPCDPPGRPEAIIVTRNSVTLQWKKPTYDGGSKITGY IVEKKELPEGRWMKASFTNIIDTHFEVTGLVEDHRYEFRVIARNAAGVFS EPSESTGAITARDEVDPPRISMDPKYKDTIVVHAGESFKVDADIYGKPIP TIQWIKGDQELSNTARLEIKSTDFATSLSVKDAVRVDSGNYILKAKNVAG ERSVTVNVKVLDRPGPPEGPVVISGVTAEKCTLAWKPPLQDGGSDIINYI VERRETSRLVWTVVDANVQTLSCKVTKLLEGNEYTFRIMAVNKYGVGEPL ESEPVVAKNPFVVPDAPKAPEVTTVTKDSMIVVWERPASDGGSEILGYVL EKRDKEGIRWTRCHKRLIGELRLRVTGLIENHDYEFRVSAENAAGLSEPS PPSAYQKACDPIYKPGPPNNPKVIDITRSSVFLSWSKPIYDGGCEIQGYI VEKCDVSVGEWTMCTPPTGINKTNIEVEKLLEKHEYNFRICAINKAGVGE HADVPGPIIVEEKLEAPDIDLDLELRKIINIRAGGSLRLFVPIKGRPTPE VKWGKVDGEIRDAAIIDVTSSFTSLVLDNVNRYDSGKYTLTLENSSGTKS AFVTVRVLDTPSPPVNLKVTEITKDSVSITWEPPLLDGGSKIKNYIVEKR EATRKSYAAVVTNCHKNSWKIDQLQEGCSYYFRVTAENEYGIGLPAQTAD PIKVAEVPQPPGKITVDDVTRNSVSLSWTKPEHDGGSKIIQYIVEMQAKH SEKWSECARVKSLQAVITNLTQGEEYLFRVVAVNEKGRSDPRSLAVPIVA KDLVIEPDVKPAFSSYSVQVGQDLKIEVPISGRPKPTITWTKDGLPLKQT TRINVTDSLDLTTLSIKETHKDDGGQYGITVANVVGQKTASIEIVTLDKP DPPKGPVKFDDVSAESITLSWNPPLYTGGCQITNYIVQKRDTTTTVWDVV SATVARTTLKVTKLKTGTEYQFRIFAENRYGQSFALESDPIVAQYPYKEP GPPGTPFATAISKDSMVIQWHEPVNNGGSPVIGYHLERKERNSILWTKVN KTIIHDTQFKAQNLEEGIEYEFRVYAENIVGVGKASKNSECYVARDPCDP PGTPEPIMVKRNEITLQWTKPVYDGGSMITGYIVEKRDLPDGRWMKASFT NVIETQFTVSGLTEDQRYEFRVIAKNAAGAISKPSDSTGPITAKDEVELP RISMDPKFRDTIVVNAGETFRLEADVHGKPLPTIEWLRGDKEIEESARCE IKNTDFKALLIVKDAIRIDGGQYILRASNVAGSKSFPVNVKVLDRPGPPE GPVQVTGVTSEKCSLTWSPPLQDGGSDISHYVVEKRETSRLAWTVVASEV VTNSLKVTKLLEGNEYVFRIMAVNKYGVGEPLESAPVLMKNPFVLPGPPK SLEVTNIAKDSMTVCWNRPDSDGGSEIIGYIVEKRDRSGIRWIKCNKRRI TDLRLRVTGLTEDHEYEFRVSAENAAGVGEPSPATVYYKACDPVFKPGPP TNAHIVDTTKNSITLAWGKPIYDGGSEILGYVVEICKADEEEWQIVTPQT GLRVTRFEISKLTEHQEYKIRVCALNKVGLGEATSVPGTVKPEDKLEAPE LDLDSELRKGIVVRAGGSARIHIPFKGRPTPEITWSREEGEFTDKVQIEK GVNYTQLSIDNCDRNDAGKYILKLENSSGSKSAFVTVKVLDTPGPPQNLA VKEVRKDSAFLVWEPPIIDGGAKVKNYVIDKRESTRKAYANVSSKCSKTS FKVENLTEGAIYYFRVMAENEFGVGVPVETVDAVKAAEPPSPPGKVTLTD VSQTSASLMWEKPEHDGGSRVLGYVVEMQPKGTEKWSIVAESKVCNAVVT GLSSGQEYQFRVKAYNEKGKSDPRVLGVPVIAKDLTIQPSLKLPFNTYSI QAGEDLKIEIPVIGRPRPNISWVKDGEPLKQTTRVNVEETATSTVLHIKE GNKDDFGKYTVTATNSAGTATENLSVIVLEKPGPPVGPVRFDEVSADFVV ISWEPPAYTGGCQISNYIVEKRDTTTTTWHMVSATVARTTIKITKLKTGT EYQFRIFAENRYGKSAPLDSKAVIVQYPFKEPGPPGTPFVTSISKDQMLV QWHEPVNDGGTKIIGYHLEQKEKNSILWVKLNKTPIQDTKFKTTGLDEGL EYEFKVSAENIVGIGKPSKVSECFVARDPCDPPGRPEAIVITRNNVTLKW KKPAYDGGSKITGYIVEKKDLPDGRWMKASFTNVLETEFTVSGLVEDQRY EFRVIARNAAGNFSEPSDSSGAITARDEIDAPNASLDPKYKDVIVVHAGE TFVLEADIRGKPIPDVVWSKDGKELEETAARMEIKSTIQKTTLVVKDCIR TDGGQYILKLSNVGGTKSIPITVKVLDRPGPPEGPLKVTGVTAEKCYLAW NPPLQDGGANISHYIIEKRETSRLSWTQVSTEVQALNYKVTKLLPGNEYI FRVMAVNKYGIGEPLESGPVTACNPYKPPGPPSTPEVSAITKDSMVVTWA RPVDDGGTEIEGYILEKRDKEGVRWTKCNKKTLTDLRLRVTGLTEGHSYE FRVAAENAAGVGEPSEPSVFYRACDALYPPGPPSNPKVTDTSRSSVSLAW SKPIYDGGAPVKGYVVEVKEAAADEWTTCTPPTGLQGKQFTVTKLKENTE YNFRICAINSEGVGEPATLPGSVVAQERIEPPEIELDADLRKVVVLRASA TLRLFVTIKGRPEPEVKWEKAEGILTDRAQIEVTSSFTMLVIDNVTRFDS GRYNLTLENNSGSKTAFVNVRVLDSPSAPVNLTIREVKKDSVTLSWEPPL IDGGAKITNYIVEKRETTRKAYATITNNCTKTTFRIENLQEGCSYYFRVL ASNEYGIGLPAETTEPVKVSEPPLPPGRVTLVDVTRNTATIKWEKPESDG GSKITGYVVEMQTKGSEKWSTCTQVKTLEATISGLTAGEEYVFRVAAVNE KGRSDPRQLGVPVIARDIEIKPSVELPFHTFNVKAREQLKIDVPFKGRPQ ATVNWRKDGQTLKETTRVNVSSSKTVTSLSIKEASKEDVGTYELCVSNSA GSITVPITIIVLDRPGPPGPIRIDEVSCDSITISWNPPEYDGGCQISNYI VEKKETTSTTWHIVSQAVARTSIKIVRLTTGSEYQFRVCAENRYGKSSYS ESSAVVAEYPFSxxxxxxxxxxVHATKSTMLVTWQVPVNDGGSRVIGYHL EYKERSSILWSKANKILIADTQMKVSGLDEGLMYEYRVYAENIAGIGKCS KSCEPVPARDPCDPPGQPEVTNITRKSVSLKWSKPHYDGGAKITGYIVER RELPDGRWLKCNYTNIQETYFEVTELTEDQRYEFRVFARNAADSVSEPSE STGPIIVKDDVEPPRVMMDVKFRDVIVVKAGEVLKINADIAGRPLPVISW AKDGIEIEERARTEIISTDNHTLLTVKDCIRRDTGQYVLTLKNVAGTRSV AVNCKVLDKPGPPAGPLEINGLTAEKCSLSWGRPQEDGGADIDYYIVEKR ETSHLAWTICEGELQMTSCKVTKLLKGNEYIFRVTGVNKYGVGEPLESVA IKALDPFTVPSPPTSLEITSVTKESMTLCWSRPESDGGSEISGYIIERRE KNSLRWVRVNKKPVYDLRVKSTGLREGCEYEYRVYAENAAGLSLPSETSP LIRAEDPVFLPSPPSKPKIVDSGKTTITIAWVKPLFDGGAPITGYTVEYK KSDDTDWKTSIQSLRGTEYTISGLTTGAEYVFRVKSVNKVGASDPSDSSD PQIAKEREEEPLFDIDSEMRKTLIVKAGASFTMTVPFRGRPVPNVLWSKP DTDLRTRAYVDTTDSRTSLTIENANRNDSGKYTLTIQNVLSAASLTLVVK VLDTPGPPTNITVQDVTKESAVLSWDVPENDGGAPVKNYHIEKREASKKA WVSVTNNCNRLSYKVTNLQEGAIYYFRVSGENEFGVGIPAETKEGVKITE KPSPPEKLGVTSISKDSVSLTWLKPEHDGGSRIVHYVVEALEKGQKNWVK CAVAKSTHHVVSGLRENSEYFFRVFAENQAGLSDPRELLLPVLIKEQLEP PEIDMKNFPSHTVYVRAGSNLKVDIPISGKPLPKVTLSRDGVPLKATMRF NTEITAENLTINLKESVTADAGRYEITAANSSGTTKAFINIVVLDRPGPP TGPVVISDITEESVTLKWEPPKYDGGSQVTNYILLKRETSTAVWTEVSAT VARTMMKVMKLTTGEEYQFRIKAENRFGISDHIDSACVTVKLPYTTPGPP STPWVTNVTRESITVGWHEPVSNGGSAVVGYHLEMKDRNSILWQKANKLV IRTTHFKVTTISAGLIYEFRVYAENAAGVGKPSHPSEPVLAIDACEPPRN VRITDISKNSVSLSWQQPAFDGGSKITGYIVERRDLPDGRWTKASFTNVT ETQFIISGLTQNSQYEFRVFARNAVGSISNPSEVVGPITCIDSYGGPVID LPLEYTEVVKYRAGTSVKLRAGISGKPAPTIEWYKDDKELQTNALVCVEN TTDLASILIKDADRLNSGCYELKLRNAMGSASATIRVQILDKPGPPGGPI EFKTVTAEKITLLWRPPADDGGAKITHYIVEKRETSRVVWSMVSEHLEEC IITTTKIIKGNEYIFRVRAVNKYGIGEPLESDSVVAKNAFVTPGPxxxxx xxxxxxNSMTVVWSRPIADGGSDISGYFLEKRDKKSLGWFKVLKETIRDT RQKVTGLTENSDYQYRVCAVNAAGQGPFSEPSEFYKAADPIDPPGPPAKI RIADSTKSSITLGWSKPVYDGGSAVTGYVVEIRQGEEEEWTTVSTKGEVR TTEYVVSNLKPGVNYYFRVSAVNCAGQGEPIEMNEPVQAKDILEAPEIDL DVALRTSVIAKAGEDVQVLIPFKGRPPPTVTWRKDEKNLGSDARYSIENT DSSSLLTIPQVTRNDTGKYILTIENGVGEPKSSTVSVKVLDTPAACQKLQ VKHVSRGTVTLLWDPPLIDGGSPIINYVIEKRDATKRTWSVVSHKCSSTS FKLIDLSEKTPFFFRVLAENEIGIGEPCETTEPVKAAEVPAPIRDLSMKD STKTSVILSWTKPDFDGGSVITEYVVERKGKGEQTWSHAGISKTCEIEVS QLKEQSVLEFRVFAKNEKGLSDPVTIGPITVKELIITPEVDLSDIPGAQV TVRIGHNVHLELPYKGKPKPSISWLKDGLPLKESEFVRFSKTENKITLSI KNAKKEHGGKYTVILDNAVCRIAVPITVITLGPPSKPKGPIRFDEIKADS VILSWDVPEDNGGGEITCYSIEKRETSQTNWKMVCSSVARTTFKVPNLVK DAEYQFRVRAENRYGVSQPLVSSIIVAKHQFRIPGPPGKPVIYNVTSDGM SLTWDAPVYDGGSEVTGFHVEKKERNSILWQKVNTSPISGREYRATGLVE GLDYQFRVYAENSAGLSSPSDPSKFTLAVSPVDPPGTPDYIDVTRETITL KWNPPLRDGGSKIVGYSIEKRQGNERWVRCNFTDVSECQYTVTGLSPGDR YEFRIIARNAVGTISPPSQSSGIIMTRDENVPPIVEFGPEYFDGLIIKSG ESLRIKALVQGRPVPRVTWFKDGVEIEKRMNMEITDVLGSTSLFVRDATR DHRGVYTVEAKNASGSAKAEIKVKVQDTPGKVVGPIRFTNITGEKMTLWW DAPLNDGCAPITHYIIEKRETSRLAWALIEDKCEAQSYTAIKLINGNEYQ FRVSAVNKFGVGRPLDSDPVVAQIQYTVPDAPGIPEPSNITGNSITLTWA RPESDGGSEIQQYILERREKKSTRWVKVISKRPISETRFKVTGLTEGNEY EFHVMAENAAGVGPASGISRLIKCREPVNPPGPPTVVKVTDTSKTTVSLE WSKPVFDGGMEIIGYIIEMCKADLGDWHKVNAEACVKTRYTVTDLQAGEE YKFRVSAINGAGKGDSCEVTGTIKAVDRLTAPELDIDANFKQTHVVRAGA SIRLFIAYQGRPTPTAVWSKPDSNLSLRADIHTTDSFSTLTVENCNRNDA GKYxxxxxxxxxxxxxxxxxxxxDTPGPPGPITFKDVTRGSATLMWDAPL LDGGARIHHYVVEKREASRRSWQVISEKCTRQIFKVNDLAEGVPYYFRVS AVNEYGVGEPYEMPEPIVATEQPAPPRRLDVVDTSKSSAVLAWLKPDHDG GSRITGYLLEMRQKGSDFWVEAGHTKQLTFTVERLVEKTEYEFRVKAKND AGYSEPREAFSSVIIKEPQIEPTADLTGITNQLITCKAGSPFTIDVPISG RPAPKVTWKLEEMRLKETDRVSITTTKDRTTLTVKDSMRGDSGRYFLTLE NTAGVKTFSVTVVVIGRPGPVTGPIEVSSVSAESCVLSWGEPKDGGGTEI TNYIVEKRESGTTAWQLVNSSVKRTQIKVTHLTKYMEYSFRVSSENRFGV SKPLESAPIIAEHPFVPPSAPTRPEVYHVSANAMSIRWEEPYHDGGSKII GYWVEKKERNTILWVKENKVPCLECNYKVTGLVEGLEYQFRTYALNAAGV SKASEASRPIMAQNPVDAPGRPEVTDVTRSTVSLIWSAPAYDGGSKVVGY IIERKPVSEVGDGRWLKCNYTIVSDNFFTVTALSEGDTYEFRVLAKNAAG VISKGSESTGPVTCRDEYAPPKAELDARLHGDLVTIRAGSDLVLDAAVGG KPEPKIIWTKGDKELDLCEKVSLQYTGKRATAVIKFCDRSDSGKYTLTVK NASGTKAVSVMVKVLDSPGPCGKLTVSRVTQEKCTLAWSLPQEDGGAEIT HYIVERRETSRLNWVIVEGECPTLSYVVTRLIKNNEYIFRVRAVNKYGPG VPVESEPIVARNSFTIPSPPGIPEEVGTGKEHIIIQWTKPESDGGNEISN YLVDKREKKSLRWTRVNKDYVVYDTRLKVTSLMEGCDYQFRVTAVNAAGN SEPSEASNFISCREPSYTPGPPSAPRVVDTTKHSISLAWTKPMYDGGTDI VGYVLEMQEKDTDQWYRVHTNATIRNTEFTVPDLKMGQKYSFRVAAVNVK GMSEYSESIAEIEPVERIEIPDLELADDLKKTVTIRAGASLRLMVSVSGR PPPVITWSKQGIDLASRAIIDTTESYSLLIVDKVNRYDAGKYTIEAENQS GKKSATVLVKVYDTPGPCPSVKVKEVSRDSVTITWEIPTIDGGAPVNNYI VEKREAAMRAFKTVTTKCSKTLYRISGLVEGTMYYFRVLPENIYGIGEPC ETSDAVLVSEVPLVPAKLEVVDVTKSTVTLAWEKPLYDGGSRLTGYVLEA CKAGTERWMKVVTLKPTVLEHTVTSLNEGEQYLFRIRAQNEKGVSEPRET VTAVTVQDLRVLPTIDLSTMPQKTIHVPAGRPVELVIPIAGRPPPAASWF FAGSKLRESERVTVETHTKVAKLTIRETTIRDTGEYTLELKNVTGTTSET IKVIILDKPGPPTGPIKIDEIDATSITISWEPPELDGGAPLSGYVVEQRD AHRPGWLPVSESVTRSTFKFTRLTEGNEYVFRVAATNRFGIGSYLQSEVI ECRSSIRIPGPPETLQIFDVSRDGMTLTWYPPEDDGGSQVTGYIVERKEV RADRWVRVNKVPVTMTRYRSTGLTEGLEYEHRVTAINARGSGKPxxxxxx xxxxxxxxxxxxxxxxxxxxxxRTSVSLAWSVPEDEGGSKVTGYLIEMQK VDQHEWTKCNTTPTKIREYTLTHLPQGAEYRFRVLACNAGGPGEPAEVPG TVKVTEMLEYPDYELDERYQEGIFVRQGGVIRLTIPIKGKPFPICKWTKE GQDISKRAMIATSETHTELVIKEADRGDSGTYDLVLENKCGKKAVYIKVR VIGSPNSPEGPLEYDDIQVRSVRVSWRPPADDGGADILGYILERREVPKA AWYTIDSRVRGTSLVVKGLKENVEYHFRVSAENQFGISKPLKSEEPVxxx xxxxxxxxxxxxxxxLDVTKSSVSLSWSRPKDDGGSRVTGYYIERKETST DKWVRHNKTQITTTMYTVTGLVPDAEYQFRIIAQNDVGLSETSPASEPVV CKDPFDKPSQPGELEILSISKDSVTLQWEKPECDGGKEILGYWVEYRQSG DSAWKKSNKERIKDKQFTIGGLLEATEYEFRVFAENETGLSRPRRTAMSI KTKLTSGEAPGIRKEMKDVTTKLGEAAQLSCQIVGRPLPDIKWYRFGKEL IQSRKYKMSSDGRTHTLTVMTEEQEDEGVYTCIATNEVGEVETSSKLLLQ ATPQFHPGYPLKEKYYGAVGSTLRLHVMYIGRPVPAMTWFHGQKLLQNSE NITIENTEHYTHLVMKNVQRKTHAGKYKVQLSNVFGTVDAILDVEIQDKP DKPTGPIVIEALLKNSAVISWKPPADDGGSWITNYVVEKCEAKEGAEWQL VSSAISVTTCRIVNLTENAGYYFRVSAQNTFGISDPLEVSSVVIIKSPFE KPGAPGKPTITAVTKDSCVVAWKPPASDGGAKIRNYYLEKREKKQNKWIS VTTEEIRETVFSVKNLIEGLEYEFRVKCENLGGESEWSEISEPITPKSDV PIQAPHFKEELRNLNVRYQSNATLVCKVTGHPKPIVKWYRQGKEIIADGL KYRIQEFKGGYHQLIIASVTDDDATVYQVRATNQGGSVSGTASLEVEVPA KIHLPKTLEGMGAVHALRGEVVSIKIPFSGKPDPVITWQKGQDLIDNNGH YQVIVTRSFTSLVFPNGVERKDAGFYVVCAKNRFGIDQKTVELDVADVPD PPRGVKVSDVSRDSVNLTWTEPASDGGSKITNYIVEKCATTAERWLRVGQ ARETRYTVINLFGKTSYQFRVIAENKFGLSKPSxxxxxTITKEDKTRAMN YDEEVDETREVSMTKASHSSTKELYEKYMIAEDLGRGEFGIVHRCVETSS KKTYMAKFVKVKGTDQVLVKKEISILNIARHRNILHLHESFESMEELVMI FEFISGLDIFERINTSAFELNEREIVSYVHQVCEALQFLHSHNIGHFDIR PENIIYQTRRSSTIKIIEFGQARQLKPGDNFRLLFTAPEYYAPEVHQHDV VSTATDMWSLGTLVYVLLSGINPFLAETNQQIIENIMNAEYTFDEEAFKE ISIEAMDFVDRLLVKERKSRMTASEALQHPWLKQKIERVSTKVIRTLKHR RYYHTLIKKDLNMVVSAARISCGGAIRSQKGVSVAKVKVASIEIGPVSGQ IMHAVGEEGGHVKYVCKIENYDQSTQVTWYFGVRQLENSEKYEITYEDGV AILYVKDITKLDDGTYRCKVVNDYGEDSSYAELFVKGVREVYDYYCRRTM KKIKRRTDTMRLLERPPEFTLPLYNKTAYVGENVRFGVTITVHPEPHVTW YKSGQKIKPGDNDKKYTFESDKGLYQLTINSVTTDDDAEYTVVARNKYGE DSCKAKLTVTLHPPPTDSTLRPMFKRLLANAECQEGQSVCFEIRVSGIPP PTLKWEKDGQPLSLGPNIEIIHEGLDYYALHIRDTLPEDTGYYRVTATNT AGSTSCQAHLQVERLRYKKQEFKSKEEHERHVQKQIDKTLRMAEILSGTE SVPLTQVAKEALREAAVLYKPAVSTKTVKGEFRLEIEEKKEERKLRMPYD VPEPRKYKQTTIEEDQRIKQFVPMSDMKWYKKIRDQYEMPGKLDRVVQKR PKRIRLSRWEQFYVMPLPRITDQYRPKWRIPKLSQDDLEIVRPARRRTPS PDYDFYYRPRRRSLGDISDEELLLPIDDYLAMKRTEEERLRLEEELELGF SASPPSRSPPHFELSSLRYSSPQAHVKVEETRKDFRYSTYHIPTKAEAST SYAELRERHAQAAYRQPKQRQRIMAEREDEELLRPVTTTQHLSEYKSELD FMSKExxxxxxxxxxxxxxxxxxxxxxxxxSKHAxxxxxxxxxxxxxxxx SLSPTYIELMRPVSELIRSRPQPAEEYEDDTERRSPTPExxxxxxxxxVS SERSLSRFERSARFDIFSRYESMKAALKTQKTSERKYEVLSQQPFTLDHA PRITLRMRSHRVPCGQNTRFILNVQSKPTAEVKWYHNGVELQESSKIHYT NTSGVLTLEILDCHTDDSGTYRAVCTNYKGEASDYATLDVTGGDYTTYAS QRRDEEVPRSVFPELTRTEAYAVSSFKKTSEMEASSxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTLAARILTKPRSMTVY EGESARFSCDTDGEPVPTVTWLRKGQVLSTSARHQVTTTKYKSTFEISSV QASDEGNYSVVVENSEGKQEAEFTLTIQKARVTEKAVTSPPRVKSxxxxx xxxxxxxxxxxxxxxxxxHxKAVSxxxxxxxxxEKVQHLPVSAPPKITQF LKAEASKEIAKLTCVVESSVLRAKEVTWYKDGKKLKENGHFQFHYSADGT YELKINNLTESDQGEYVCEISGEGGTSKTNLQFMGQAFKSIHEKVSKISE xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxVTGLQDTTVSSDSVAKFAV KATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYT CTVKNSAGSVSSSCKLTIKAIKDTEAQKVSTQKTSEITPQKKAVVQEEIS QxxxxxxxxxxxxxxxxxKLAxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxVRTHAEIKAFSTQMSINEGQRLVLKANIAGATDVKWVLN GVELTNSEEYRYGVSGSDQTLTIKQASHRDEGILTCISKTKEGIVKCQYD LTLSKELSDAPAFISQPRSQNINEGQNVLFTCEISGEPSPEIEWFKNNLP ISISSxxxxxxxxxVYSLEIRNASVSDSGKYTIKAKNFRGQCSATASLMV LPLVEEPSREVVLRTSGDTSLQGSFSSQSVQMSASKxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxLKAG IRGIPPKIEALPSDISIDEGKVLTVACAFTGEPTPEVTWSCGGRKIHSQE QGRFHIENTDDLTTLIIMDVQKQDGGLYTLSLGNEFGSDSATVNIHIRSI #period estimated number of tracts 1 11.5539 2 12.071 3 23.5112 4 8.6641 5 10.2403 6 10.2883 7 11.3853 8 11.585 9 4.50516 10 4.75721 11 8.23821 12 3.43443 13 4.34498 14 2.43048 15 6.08299 16 5.03573 17 3.15392 18 1.90906 19 2.38249 20 1.69083 21 1.64297 22 1.36296 23 1.42971 24 1.31135 25 3.43442 26 5.87207 27 2.84509 28 5.23273 29 0.737526 30 0.733329 31 1.04262 32 0.994512 33 0.406413 34 0.253399 35 0.214558 36 1.24664 37 0.817335 38 0.361951 39 0.299148 40 0.221853 41 0.147372 42 1.56514 43 0.211522 44 0.0943722 45 0.327042 46 2.10489 47 0.0983897 48 0.488297 49 0.0632472 50 0.0948114 # estimated total number of repetitive tracts: 182.92 # estimated number of background-to-background transitions: 30295.5 # total number of transitions (#letters + #sequences): 34351 # best-fit probability decay per period: 0.914731 # best-fit probability of a repeat starting per position: 0.00600163 # best-fit probability of a repeat ending per position: 0.0472345 817 234 934 3615 @SRR019778.1 I331_2_FC3076HAAXX:1:1:1416:1963 length=45 GTTCAATGGGGGAAAGGAGAGTCCTTCAACAAATGGTGCGGAGAA +SRR019778.1 I331_2_FC3076HAAXX:1:1:1416:1963 length=45 IIIIIIIIIIIIIIIIIIIIIIIIIIIB2IHH(-0+ @SRR019778.3 I331_2_FC3076HAAXX:1:1:1611:1333 length=45 GTTTTCCCATTTGTCCTTAATCCACCTAAAAAAGATTTGCATGGG +SRR019778.3 I331_2_FC3076HAAXX:1:1:1611:1333 length=45 IIIIIIIIIIIIIEIIIIG:IDIGECI454,10+0A?8/4+40%( @SRR019778.4 I331_2_FC3076HAAXX:1:1:1277:1927 length=45 GTTGtgtgtgtattgtgtgttgtgtgtggtgtgtgtgtttttttt +SRR019778.4 I331_2_FC3076HAAXX:1:1:1277:1927 length=45 IIIIIIIIIIIIII6IIIIIIDIII4I%4I*I0IAI+II8/3I+I @SRR019778.5 I331_2_FC3076HAAXX:1:1:1494:1984 length=45 GGATGTTTATTTTCCTGAACCAGGGTTTGGCATTGAAGAGACAGA +SRR019778.5 I331_2_FC3076HAAXX:1:1:1494:1984 length=45 IIIIIIIIIIIIIIIIIIIII/III@III@<-I6I'3I67-++>) @SRR019778.6 I331_2_FC3076HAAXX:1:1:682:1158 length=45 GTTTGTTTCCTAGAGTTAAGAGTCTCTCATGGTTTGTCTCCCTCT +SRR019778.6 I331_2_FC3076HAAXX:1:1:682:1158 length=45 IIIIIIIIIIIII:IIII=IEIIHI@IC2I9;I@@//?4DA5080,&, @SRR019778.13 I331_2_FC3076HAAXX:1:1:1245:2003 length=45 GTTTCCATAACCAGAAATATACAGCGGATTAGAGAAGTGATTTTT +SRR019778.13 I331_2_FC3076HAAXX:1:1:1245:2003 length=45 IIIIIIIIIIIIIIG>1I4I5I9IIII*II.I+G)-B7G&:B@C@ @SRR019778.14 I331_2_FC3076HAAXX:1:1:1226:1990 length=45 GGGAGGATCTAGCCTTAGAACCAAGAAACAAAATGTGAGGGGAAA +SRR019778.14 I331_2_FC3076HAAXX:1:1:1226:1990 length=45 IIIIIIIIIIIIIIII6IIIIIAII>.III-3/II3I1IIII.-- @SRR019778.15 I331_2_FC3076HAAXX:1:1:1879:1017 length=45 GTGATGTGCCATTGACCTATTTGTTTATCTTTATACCAATACTTC +SRR019778.15 I331_2_FC3076HAAXX:1:1:1879:1017 length=45 IIIIIIIIIIIIII2III/IIDGFII/I7IG:5CC;/0&=)+;4+ @SRR019778.16 I331_2_FC3076HAAXX:1:1:1478:1340 length=45 GTATTTTACAGAAGTTTCTCTGGGTCTCAGATTCCTACCTGACCC +SRR019778.16 I331_2_FC3076HAAXX:1:1:1478:1340 length=45 IIIIIIIIIIIIIIDIIIIII>;C;DI@D*3IE<2B/20;&'/-/ @SRR019778.17 I331_2_FC3076HAAXX:1:1:1489:1981 length=45 GGGAAAGATAGAGAGCCTTGTCTTCAAAATGGCTTATCCAGTAAG +SRR019778.17 I331_2_FC3076HAAXX:1:1:1489:1981 length=45 IIIIIIIIIIIIIIIIIIIIIIIII?+<5IIIIIB*66<%=3'(5 @SRR019778.18 I331_2_FC3076HAAXX:1:1:1710:940 length=45 GAGAATCCACAAGAAGTTGCTGTTAGGGATTTGTTCTTATTTTAA +SRR019778.18 I331_2_FC3076HAAXX:1:1:1710:940 length=45 IIIIIIIIIIIII0CIIIIIII@I(-?6-@?C.37,A:%D;:&** @SRR019778.19 I331_2_FC3076HAAXX:1:1:1339:973 length=45 GAAAAGGCATAAATATTTCATTCAATTGCAAGCTTAATATAAGTC +SRR019778.19 I331_2_FC3076HAAXX:1:1:1339:973 length=45 IIIIIIIIIIIIIIFIIIIHIIII:II;II63=IIC.GFI76(.. @SRR019778.20 I331_2_FC3076HAAXX:1:1:1408:1952 length=45 GAGTTTTCCATTTAAGAACTTGAGTGTCATCGCCTTAGGAAAGAC +SRR019778.20 I331_2_FC3076HAAXX:1:1:1408:1952 length=45 IIIIIIIIIIIII@FIFGIIII8I?+HIIDI=E>I>8?1)1&*// @SRR019778.21 I331_2_FC3076HAAXX:1:1:1468:1371 length=45 GAAGAGAAAGGAGATACCATCATTTGTCATTAACAGGATTACCTT +SRR019778.21 I331_2_FC3076HAAXX:1:1:1468:1371 length=45 IIIIIIIIIIIIIIIIIIIIIFIIII=I3I:13=A<@,/03I2<+:- @SRR019778.26 I331_2_FC3076HAAXX:1:1:1225:1945 length=45 GTAATGATTGAATAAATCTTATTAActtcttaatcttttaatTTT +SRR019778.26 I331_2_FC3076HAAXX:1:1:1225:1945 length=45 IIIIIIIIIIIIIIIIIIIIIIIB4HIIIII:3IIIIII5)IIGI @SRR019778.27 I331_2_FC3076HAAXX:1:1:1506:1294 length=45 GCTTCAAGAAGAAGGGCATGACTCACCCCCCAGGAACAGAAGCTG +SRR019778.27 I331_2_FC3076HAAXX:1:1:1506:1294 length=45 IIIIIIIIIIIIIIIIIIIIIIII@IIICDB15B+39.9*.B4:5 @SRR019778.28 I331_2_FC3076HAAXX:1:1:1318:1418 length=45 GATTTTATTCTTATTGTTTTGATGGTGACCTTTAATATTTTCTTA +SRR019778.28 I331_2_FC3076HAAXX:1:1:1318:1418 length=45 IIIIIIIIIIIIIIIIIIIIADI,BFIA..IIIC2$7IFEC1&;) @SRR019778.29 I331_2_FC3076HAAXX:1:1:1332:1930 length=45 GAGAATAAGTCAATACCAAGGTGAATTGAATAATGGCCAAGAATT +SRR019778.29 I331_2_FC3076HAAXX:1:1:1332:1930 length=45 IIIIIIIIIIIIIIIIGIHIIII58IIII5I@9AI8I0=,B,-3> @SRR019778.30 I331_2_FC3076HAAXX:1:1:1099:1168 length=45 GTTTATTCTCTATCATTTAGAGTCTCAGCTGTTTGGGTTTCTAGA +SRR019778.30 I331_2_FC3076HAAXX:1:1:1099:1168 length=45 IIIIIIIIIIIIII;IIII8I+BFII+C.2+8I.,&%3$$3/$)$ @SRR019778.31 I331_2_FC3076HAAXX:1:1:1529:1292 length=45 GGATAGCCCCAAGTGTTACAAATTGTAAGTAAATATTTGTAGGGA +SRR019778.31 I331_2_FC3076HAAXX:1:1:1529:1292 length=45 IIIIIIIIIIIIIIIII>I<<8IIIID2I=/7.;2EH@22/6+&* @SRR019778.32 I331_2_FC3076HAAXX:1:1:1256:1996 length=45 GTTGGGGATTAGAAAGTAGATGTAAGGAGTAGTAAAGGGAATACA +SRR019778.32 I331_2_FC3076HAAXX:1:1:1256:1996 length=45 IIIIIIIIIIIIIHIIIIIIIIHI6II5I;I3 @SRR019778.39 I331_2_FC3076HAAXX:1:1:1136:1244 length=45 GTTATATGTGAGTAATGAACCATTGAACACTACACCAAAAATTAA +SRR019778.39 I331_2_FC3076HAAXX:1:1:1136:1244 length=45 IIIIIIIIIIIIIIIIIIIIDIIIII:8B8IH3;72<8031D92- @SRR019778.40 I331_2_FC3076HAAXX:1:1:1819:927 length=45 GGGTTTGGAGTTTAAAGAGAGAATAAGTCATGTGCCAATATATGA +SRR019778.40 I331_2_FC3076HAAXX:1:1:1819:927 length=45 IIIIIIIIIIIIIIG;IFIIII6II?IEBE?8@A<79,D5<4F1) @SRR019778.41 I331_2_FC3076HAAXX:1:1:1250:1938 length=45 GTTTGTGTCCCTCTAAAGTTCATATACTGAAGCCCTAACCCCCAG +SRR019778.41 I331_2_FC3076HAAXX:1:1:1250:1938 length=45 IIIIIIIIIIIIIIII2IIIIIIIIIIII=:=IIEI3/I7I7G+0 @SRR019778.42 I331_2_FC3076HAAXX:1:1:1648:1646 length=45 GGTTGTgtgtgtgtgtgtgtgtgtgtgtgtttgtttttttttttA +SRR019778.42 I331_2_FC3076HAAXX:1:1:1648:1646 length=45 IIIIIIIIIIIIIIIIIIII7I@I.IDIB,.@%I0*-E(G%(/.% @SRR019778.43 I331_2_FC3076HAAXX:1:1:690:962 length=45 GTGAGATGATGTTCGTTGATATTTCCCTGTATTTTTTGTTTTTTC +SRR019778.43 I331_2_FC3076HAAXX:1:1:690:962 length=45 IIIIIIIIIIIIIIIIII>I?III??,I'$)II%:&B&'8(9$=$ @SRR019778.44 I331_2_FC3076HAAXX:1:1:1399:1957 length=45 GTTGATATTTCCCTGGACTGTGTGGGGTCTCAGAGACCACTATGG +SRR019778.44 I331_2_FC3076HAAXX:1:1:1399:1957 length=45 IIIIIIIIIIIIIIII=IIIIIIIIIIIIIG4>,I/9;*3D*:<+ @SRR019778.45 I331_2_FC3076HAAXX:1:1:1797:1797 length=45 GTgtgtattgtgtgtgggtgtgtgtgttgtgtgtgttttGGGTGG +SRR019778.45 I331_2_FC3076HAAXX:1:1:1797:1797 length=45 IIIIIIIIIIIIII@IIIIIIIAII?II?I0(+D)II$:&5%'&% @SRR019778.46 I331_2_FC3076HAAXX:1:1:721:921 length=45 GATGTCATAGAAGTTGTTGCCTATGTTCTCCTCTAGAATTTTGAT +SRR019778.46 I331_2_FC3076HAAXX:1:1:721:921 length=45 IIIIIIIIIIIIIIIIIIH?CI6I7II0I11F5I+&+(ICIH&&= @SRR019778.47 I331_2_FC3076HAAXX:1:1:1584:1300 length=45 GGTAGTGAGTATCAGCCCTGTGCAGGTTTCTGCAGATGGCCATGT +SRR019778.47 I331_2_FC3076HAAXX:1:1:1584:1300 length=45 IIIII-IIIIIIIHIIIII4;GCIEI-II320-&-#)%,.,$-%$ @SRR019778.48 I331_2_FC3076HAAXX:1:1:1397:1963 length=45 GAAAACTTACTTACAAATTCATTCTATGAGGCCAGCATTACCCTG +SRR019778.48 I331_2_FC3076HAAXX:1:1:1397:1963 length=45 IIIIIIIIIIIIIIII:IIIIIIII4II4FIIH0F@*CI47><:1 @SRR019778.49 I331_2_FC3076HAAXX:1:1:1550:1296 length=45 GGTAGATGTGACTCTTGATCTCAGGGTCATGAGTTCAAGCCCCAT +SRR019778.49 I331_2_FC3076HAAXX:1:1:1550:1296 length=45 IIIIIIIIIIIIIIIIICIIBI6D%I9B5C;0I+;37-4)0**)3 @SRR019778.50 I331_2_FC3076HAAXX:1:1:913:1939 length=45 GTGtgttgtgtgttgagtgttttgtgtgtgtgttgtgttttttGT +SRR019778.50 I331_2_FC3076HAAXX:1:1:913:1939 length=45 IIIIIIIIIIIIIII2IIIIIII@IIG2I.I,II&9&4%II,:%, @SRR019778.51 I331_2_FC3076HAAXX:1:1:1734:1009 length=45 GAAACAATGGTTTTCAAGACACTGGACATCAGCCTACAATGGACA +SRR019778.51 I331_2_FC3076HAAXX:1:1:1734:1009 length=45 IIIIIIIIIIIIIIIIIIEIBIIID.>2I?186C?)/&-8,1)1+ @SRR019778.52 I331_2_FC3076HAAXX:1:1:1355:100 length=45 GTACACAAATCTACGTGATATTTCGAAAGGTAAACAGATAATGCT +SRR019778.52 I331_2_FC3076HAAXX:1:1:1355:100 length=45 IIIIIIIIIIIII7I7,IIC&FD>1->9'+00 @SRR019778.56 I331_2_FC3076HAAXX:1:1:1759:1586 length=45 GGTACTCTAATAGCGTTTTATGGTAAGAGATGGTAGCTGTACTTG +SRR019778.56 I331_2_FC3076HAAXX:1:1:1759:1586 length=45 IIIIIIIIIF0I1III++;6.':594*7)4* @SRR019778.58 I331_2_FC3076HAAXX:1:1:1371:1534 length=45 GTGATGGGAAAGGAAAGAGCCAATCCTGTAGGGCCTGGTAGATCA +SRR019778.58 I331_2_FC3076HAAXX:1:1:1371:1534 length=45 I7IIIIIIIIIIIIIIIIIIII>III@*86IIIF??I7+)6-9+0 @SRR019778.59 I331_2_FC3076HAAXX:1:1:1780:1771 length=45 GTAAAATGGAGTAAATAGAGTAGAATAACATAAAAATTACAATGT +SRR019778.59 I331_2_FC3076HAAXX:1:1:1780:1771 length=45 IIIIIIIIIIIII;IIIE.-/0827:4./3- @SRR019778.60 I331_2_FC3076HAAXX:1:1:1539:1636 length=45 GTGTTGCTCTTTCTGAGGTGGTAATTGTGGTTTCCTTGGGCCTTT +SRR019778.60 I331_2_FC3076HAAXX:1:1:1539:1636 length=45 IIIIIIIIIIIIIIIIIICII:7III9I9IFII1(4I%%+&*1&. @SRR019778.61 I331_2_FC3076HAAXX:1:1:1360:1444 length=45 GTTGGGTTGATTTTCATTGGTGCTAATGTAATGGGGTATTGATCC +SRR019778.61 I331_2_FC3076HAAXX:1:1:1360:1444 length=45 IIIIIIIIIIIIIIIIIIIBI/GI>1II%).I),,+9'IB)+B+) @SRR019778.62 I331_2_FC3076HAAXX:1:1:1763:997 length=45 GAGATGAGTCAGCAGAGGTAAAAGCTAGAAGGATGTTAGGATGAT +SRR019778.62 I331_2_FC3076HAAXX:1:1:1763:997 length=45 IIIIIIIIIIIII?I@IID=II@A?E?I1;D=:C@6>-<0+A2.= @SRR019778.63 I331_2_FC3076HAAXX:1:1:996:1270 length=45 GTCAGGGACTTAATATCTTTCCCTTAAAATATGTGTGTTATTTTC +SRR019778.63 I331_2_FC3076HAAXX:1:1:996:1270 length=45 IIIIIIIIIIIIIIIIIIIIIEIII<:9)<(:I%: @SRR019778.66 I331_2_FC3076HAAXX:1:1:1314:76 length=45 GTGCTTAGCAGATAGGAGGGGCTCATTAACATGTTGAACAAATGT +SRR019778.66 I331_2_FC3076HAAXX:1:1:1314:76 length=45 IIIIIIIIIIIII7II7IIII?II4II/14+I*I@/.:/.6*F>>+ @SRR019778.68 I331_2_FC3076HAAXX:1:1:1459:1955 length=45 GTGTTCCCATACTTTAACTTGtgtgtgttgtggggggAAGCATAT +SRR019778.68 I331_2_FC3076HAAXX:1:1:1459:1955 length=45 IIIIIIIIDIIIIII>FIIIIIIIIIIII6I>47>I+*(/#$I$@ @SRR019778.69 I331_2_FC3076HAAXX:1:1:1381:1967 length=45 GCCCTAAGAACCAAGAAACAAAGTGTGAGGGGAAAGCCCTTGAAT +SRR019778.69 I331_2_FC3076HAAXX:1:1:1381:1967 length=45 IIIIIIIIIIIII:IE)CIC?1F4I+I8IIII.,/I959-:B&'9 @SRR019778.70 I331_2_FC3076HAAXX:1:1:1224:2001 length=45 GATACGTCAAATTTATGGAAGTGTTTTATAAGTGCATTTAAGTTT +SRR019778.70 I331_2_FC3076HAAXX:1:1:1224:2001 length=45 IIIIIIIIIIIIIIIIII8EI=IIIII8I4.9E9+$II&%+(:?& @SRR019778.71 I331_2_FC3076HAAXX:1:1:1771:1569 length=45 GTTTTTATAATGACTTCCTTAGGCTGTACCCCCAATTTACTATAT +SRR019778.71 I331_2_FC3076HAAXX:1:1:1771:1569 length=45 IIIIIIIIIIIIIIIIIIII*(IAB(4?'D'F @SRR019778.72 I331_2_FC3076HAAXX:1:1:1745:1606 length=45 GATTTGCAGTCAAGGACAGGTCCACACAGTCAGGACCACAGTGTT +SRR019778.72 I331_2_FC3076HAAXX:1:1:1745:1606 length=45 IIIIIIIIIIIIEIIII-I2; @SRR019778.73 I331_2_FC3076HAAXX:1:1:1279:1959 length=45 GGAGGAAGACCACAGAGCTGAAGCACCGTCCTCATCACATCAGGT +SRR019778.73 I331_2_FC3076HAAXX:1:1:1279:1959 length=45 IIIIIIIIIIIIIIIIIIIIHFII86II6IICI1CI4I4*5*4B( @SRR019778.74 I331_2_FC3076HAAXX:1:1:1769:1745 length=45 GAAGTGATGTAGATGCCTATATGAATACAGGCACATATGGTTTCC +SRR019778.74 I331_2_FC3076HAAXX:1:1:1769:1745 length=45 IIIIDIIIIIIIIIIIII7IIIIG>I0E2II=+7/:+7DA04*'7 @SRR019778.75 I331_2_FC3076HAAXX:1:1:721:1248 length=45 GACAAGTTTAGGAAGCCTGTTATTTTACTACATGAAACATTCATA +SRR019778.75 I331_2_FC3076HAAXX:1:1:721:1248 length=45 IIEIIIIIIIIIIIIIIII>IIIIII>III9AI+75>76?84-2I @SRR019778.76 I331_2_FC3076HAAXX:1:1:1306:616 length=45 GTTAAGATAAATCACAGAGCACCAACCTCAAGCGATGGCAGCTGC +SRR019778.76 I331_2_FC3076HAAXX:1:1:1306:616 length=45 IIIIIIIIIIIIIIIIIIIIIIIIAIC=B6:57:274.2,+(+%& @SRR019778.77 I331_2_FC3076HAAXX:1:1:1474:1625 length=45 GTAAAGGACTTTCAGGACCTAATTTAAATGATCACTCAGGCTGAG +SRR019778.77 I331_2_FC3076HAAXX:1:1:1474:1625 length=45 IIIIIIIIIIIIIIIIIIII>9III@7GII3IIFIIA34'1FA)7 @SRR019778.78 I331_2_FC3076HAAXX:1:1:1866:942 length=45 GACAGATACTGCCTTACTAtgccttactatgTGCACATATACACA +SRR019778.78 I331_2_FC3076HAAXX:1:1:1866:942 length=45 IIIIIIIIIIIIIIIIII5IIIIII1II.I+:,C33,?/;,,)/) @SRR019778.79 I331_2_FC3076HAAXX:1:1:1803:1776 length=45 GTAATATATAAACAGCATAGATAATATCAGATAAAAGGACTTTCT +SRR019778.79 I331_2_FC3076HAAXX:1:1:1803:1776 length=45 IIIIIIIIIIIII:II8IDI;I9:I/=E;I>AA0132/29>=/15 @SRR019778.80 I331_2_FC3076HAAXX:1:1:716:927 length=45 GCAAGGTAGGGACACGGGGACTCAGACAGATGAACAGATTAGACT +SRR019778.80 I331_2_FC3076HAAXX:1:1:716:927 length=45 IIIIIIIIIIIIIIIIIIIACIGBI=?2I6DA8;3+;/5:/./+1 @SRR019778.81 I331_2_FC3076HAAXX:1:1:1294:71 length=45 GTAAACATGGAACACAACAAAAAAACTTCAAAAAGGCAAAACTAG +SRR019778.81 I331_2_FC3076HAAXX:1:1:1294:71 length=45 IIIIIIIIIIIIIDIIII93I@AE.HE7I632-,63?72/1476, @SRR019778.82 I331_2_FC3076HAAXX:1:1:1486:1393 length=45 GATTTAAACAATTACATCAAAATGCATTGATTCAATTGTTTTTTA +SRR019778.82 I331_2_FC3076HAAXX:1:1:1486:1393 length=45 IIIIIIIIIIIIIIIIIIIBIIIID9:I@;4@8*B0I4A77 @SRR019778.83 I331_2_FC3076HAAXX:1:1:1880:1764 length=45 GTAAAAATAACTACATTAACTGTGACAAGGGATACCCAAGAAGAT +SRR019778.83 I331_2_FC3076HAAXX:1:1:1880:1764 length=45 IIIIIIIIIIIIIIIIIIIIIIAI:I8:IHI.52IG4'/4-+2)0 @SRR019778.84 I331_2_FC3076HAAXX:1:1:1410:1972 length=45 GCATGTGGATATCCAGTTTTCCCATTTCTTGAAAAGTGTGTCCTT +SRR019778.84 I331_2_FC3076HAAXX:1:1:1410:1972 length=45 IIIIIIIIIIIIII/IIIIIIII7IIIIII;3.)1=<<8-D86G> @SRR019778.85 I331_2_FC3076HAAXX:1:1:1681:1342 length=45 GTAGAAATGACCTTAGCCCACTATACTAGAAACTTCGGGTTTGTG +SRR019778.85 I331_2_FC3076HAAXX:1:1:1681:1342 length=45 IIIIIIIIIIIIIIIIIIFDII3IFIII)712145+.5,1&3+ @SRR019778.87 I331_2_FC3076HAAXX:1:1:1395:1991 length=45 GTGCTATCTCATCATGGTTTTGATCTGTATTTCCCTGATAATGAG +SRR019778.87 I331_2_FC3076HAAXX:1:1:1395:1991 length=45 IIIIIIIIIIIII?IIIIIIII2IIIIF7IIIGCEI;+D&-B+** @SRR019778.88 I331_2_FC3076HAAXX:1:1:1332:1959 length=45 GATAGTAACTCCTGTCTTCCTACGGGGTTAAAAAAAGCCTTCTGC +SRR019778.88 I331_2_FC3076HAAXX:1:1:1332:1959 length=45 IIIIIIIIIIIIIIIIIIII3IIIIII=ID-2/+824<;4?A872 @SRR019778.89 I331_2_FC3076HAAXX:1:1:1304:77 length=45 GAAGAGAGAAGTCTGGGGGATCAGAGGCCACCAAAAGCAAAACAA +SRR019778.89 I331_2_FC3076HAAXX:1:1:1304:77 length=45 IIIIIIIIIIIIIIICIDI?D97957@;+839,))+5-2*')**) @SRR019778.90 I331_2_FC3076HAAXX:1:1:1478:1372 length=45 GTTAAAGGTGAAGAAGGAAGAGAAGTTCCTTCCATTTGGCAGATT +SRR019778.90 I331_2_FC3076HAAXX:1:1:1478:1372 length=45 IIIIIIIIIIIIIIIIIIIIII2AI3II>IB@50A:3<*5+@):2 @SRR019778.91 I331_2_FC3076HAAXX:1:1:1518:630 length=45 GTAGTGGTGAAAGTATTTATTTTTCCCTATATCTTTGTTAATTGC +SRR019778.91 I331_2_FC3076HAAXX:1:1:1518:630 length=45 IIIIIIIIIIIIICIIII@IIIIIIDFI?I1I9IHC$:B.+BI%) @SRR019778.92 I331_2_FC3076HAAXX:1:1:1486:1973 length=45 GAAACAGACAAAAGCCTTAGTAACCTGGGGCTCAGCAAAAGGCCA +SRR019778.92 I331_2_FC3076HAAXX:1:1:1486:1973 length=45 IIIIIIIIIIIIIIIIIIIIIH?IIIIIIIIII3IH-//(I??I3 @SRR019778.93 I331_2_FC3076HAAXX:1:1:1296:1435 length=45 GAGTTTTGAACTCAGGCTGTTCTGGCCTCAAAATTCATGCTCATT +SRR019778.93 I331_2_FC3076HAAXX:1:1:1296:1435 length=45 IIIIIIIIIIIIIIIIIIIII4@&;74C1)01/9I)'9&-7+(29 @SRR019778.94 I331_2_FC3076HAAXX:1:1:1502:1287 length=45 GCACAGATGAGCCAAGAAGGGTTCCAGTTCCCCTGCTTCATTATG +SRR019778.94 I331_2_FC3076HAAXX:1:1:1502:1287 length=45 IIIIIIIIIIIIII;'*B63'20,%6- @SRR019778.96 I331_2_FC3076HAAXX:1:1:1878:956 length=45 GATAATATACTATGGCTCTGCAAGATAGTACTTGGGGGAAACAAA +SRR019778.96 I331_2_FC3076HAAXX:1:1:1878:956 length=45 IIIIIIIIIIIIIIIIII(/:*--01+1.2.-$/+0&6#,2&(&4 @SRR019778.97 I331_2_FC3076HAAXX:1:1:608:1209 length=45 GAAATTTTCCTTCAATAAAGGGAGATTCTAATCTACATATTTTAA +SRR019778.97 I331_2_FC3076HAAXX:1:1:608:1209 length=45 IIIIIIIIIIIIIIIIHH4I=Ahard catcatcatcatcat atcatatcatatcatatcatatcat tantan-22/test/hg19_chrM.fa0000644000000000000000000004101513406427735014306 0ustar rootroot>chrM GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCAT TTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTG GAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATT CTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACCTACTA AAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAACAATTGAAT GTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCA AACCCCCCCCTCCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGC CAAACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAAT TTTATCTTTAGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACACA TTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATACAACCCCC GCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAAC CAACCAAACCCCAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCA AAGCAATACACTGAAAATGTTTAGACGGGCTCACATCACCCCATAAACAA ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC AAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAG GGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCC ACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGT TTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACC GCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTT TAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAA CTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAAC ACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGC CCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGA GCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGA GGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGC TCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGT AAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGG TGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTT ATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGT GCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCC TCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATA GAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACG AACCAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTT CAACTTAACTTGACCGCTCTGAGCTAAACCTAGCCCCAAACCCACTCCAC CTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGG CGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGA TGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTT CTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAA GACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCC GTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCT ACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTT TAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAG TCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGA GTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGA AAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACT GAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAA TGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCA GATCAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA ACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAG GAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAACCTTACCCCGCCTGTT TACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCC CAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAaaggtagca taatcacttgttccttaaatagggacctgtatgaatggctccacgagggt tcagctgtctcttacttttaaccagtgaaattgacctgcccgtgaagagg cgggcatgacacagcaagacgagaagaccctatggagctttaatttaTTA ATGCAAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCA TTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCA GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT CCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATC CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGAT CAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA TTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTT CTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAGGCC TACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTAT TATACCCACACCCACCCAAGAACAGGGTTTgttaagatggcagagcccgg taatcgcataaaacttaaaactttacagtcagaggttcaattcctcttct taacaacaTACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAA TCGCAATGGCATTCCTAATGCTTACCGAACGAAAAATTCTAGGCTATATA CAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTACAACC CTTCGCTGACGCCATAAAACTCTTCACCAAAGAGCCCCTAAAACCCGCCA CATCTACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTCACCATC GCTCTTCTACTATGAACCCCCCTCCCCATACCCAACCCCCTGGTCAACCT CAACCTAGGCCTCCTATTTATTCTAGCCACCTCTAGCCTAGCCGTTTACT CAATCCTCTGATCAGGGTGAGCATCAAACTCAAACTACGCCCTGATCGGC GCACTGCGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCAT CATTCTACTATCAACATTACTAATAAGTGGCTCCTTTAACCTCTCCACCC TTATCACAACACAAGAACACCTCTGATTACTCCTGCCATCATGACCCTTG GCCATAATATGATTTATCTCCACACTAGCAGAGACCAACCGAACCCCCTT CGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGCTTCAACATCGAAT ACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATACACAAACATT ATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGA CGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTAC TTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCCGC TACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCT AGCATTACTTATATGATATGTCTCCATACCCATTACAATCTCCAGCATTC CCCCTCAAACCTAAGAAATATGTCTGATAAAAGAGTTACTTTGATAGAGT AAATAATAGGAGCTTAAACCCCCTTATTTctaggactatgagaatcgaac ccatccctgagaatccaaaattctccgtgccacctatcacaccccatcct aAAGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTG GTTATACCCTTCCCGTACTAATTAATCCCCTGGCCCAACCCGTCATCTAC TCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATT TTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTC TAACCAAAAAAATAAACCCTCGTTCCACAGAAGCTGCCATCAAGTATTTC CTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAA CAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACT CATCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGCCCCC TTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACATCCGG CCTGCTTCTTCTCACATGACAAAAACTAGCCCCCATCTCAATCATATACC AAATCTCTCCCTCACTAAACGTAAGCCTTCTCCTCACTCTCTCAATCTTA TCCATCATAGCAGGCAGTTGAGGTGGATTAAACCAAACCCAGCTACGCAA AATCTTAGCATACTCCTCAATTACCCACATAGGATGAATAATAGCAGTTC TACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATC CTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGAC CCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAA TTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTT TTGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATAGCCTCAT CATCCCCACCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTACC TACGCCTAATCTACTCCACCTCAATCACACTACTCCCCATATCTAACAAC GTAAAAATAAAATGACAGTTTGAACATACAAAACCCACCCCATTCCTCCC CACACTCATCGCCCTTACCACGCTACTCCTACCTATCTCCCCTTTTATAC TAATAATCTTATAGAAATTTAGGTTAAATACAGACCAAGAGCCTTCAAAG CCCTCAGTAAGTTGCAATACTTAATTTCTGCAACAGCTAAGGACTGCAAA ACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAA GCCCTTACTAGACCAATGGGACTTAAACCCACAAACACTTAGTTAACAGC TAAGCACCCTAATCAACTGGCTTCAATCTACTTCTCCCGCCGCCGGGAAA AAAGGCGGGAGAAGCCCCGGCAGGTTTGAAGCTGCTTCTTCGAATTTGCA ATTCAATATGAAAATCACCTCGGAGCTGGTAAAAAGAGGCCTAACCCCTG TCTTTAGATTTACAGTCCAATGCTTCACTCAGCCATTTTACCTCACCCCC ACTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTG GAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCT CTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGG TAACGACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAA TCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTA GTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAA CATAAGCTTCTGACTCTTACCTCCCTCTCTCCTACTCCTGCTCGCATCTG CTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA GCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTC CTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCA CAACAATTATCAATATAAAACCCCCTGCCATAACCCAATACCAAACGCCC CTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT CCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA ACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAA CACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACC AGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAG AACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTC CTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGT AGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCA CCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATG AAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCAC CGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCG TACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCA ATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCT ATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTA TCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGC CTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCAC ATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAA TATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTC CTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCC CCCACCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAaa aaaggaaggaatcgaaccccccaaagctggtttcaagccaaccccatggc ctccatgactttttcAAAAAGGTATTAGAAAAACCATTTCATAACTTTGT CAAAGTTAAATTATAGGCTAAATCCTATATATCTTAATGGCACATGCAGC GCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCA CCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTC CTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACAT CTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCA TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC GAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCCACCAATGGTA CTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACA TACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTT GACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTAC ATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAA CAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCGCTACACGA CCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAG TTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAG GGCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCCACTG TAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAGATTAAGAGAACCAAC ACCTCTTTACAGTGAAATGCCCCAACTAAATACTACCGTATGGCCCACCA TAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAAATA TTAAACACAAACTACCACCTACCTCCCTCACCAAAGCCCATAAAAATAAA AAATTATAACAAACCCTGAGAACCAAAATGAACGAAAATCTGTTCGCTTC ATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTC TATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGA CTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATGAT AGCCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCT TAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCA TTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTT ATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCC TAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTA GTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGT ACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTG GAAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATC ATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTT AATCCAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCACGACA ACACATAATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC CATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTA GCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGCCTACT AACCAACACACTAACCATATACCAATGGTGGCGCGATGTAACACGAGAAA GCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATAC GGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTT CTGAGCCTTTTACCACTCCAGCCTAGCCCCTACCCCCCAACTAGGAGGGC ACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTC CTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCA CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA TTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTAC TTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTT TGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCC TCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACAT CACTTTGGCTTCGAAGCCGCCGCCTGATACTGGCATTTTGTAGATGTGGT TTGACTATTTCTGTATGTCTCCATCTATTGATGAGGGTCTTACTCTTTTA GTATAAATAGTACCGTTAACTTCCAATTAACTAGTTTTGACAACATTCAA AAAAGAGTAATAAACTTCGCCTTAATTTTAATAATCAACACCCTCCTAGC CTTACTACTAATAATTATTACATTTTGACTACCACAACTCAACGGCTACA TAGAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCC CGCGTCCCTTTCTCCATAAAATTCTTCTTAGTAGCTATTACCTTCTTATT ATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCATGAGCCCTACAAA CAACTAACCTGCCACTAATAGTTATGTCATCCCTCTTATTAATCATCATC CTAGCCCTAAGTCTGGCCTATGAGTGACTACAAAAAGGATTAGACTGAGC CGAATTGGTATATAGTTTAAACAAAACGAATGATTTCGACTCATTAAATT ATGATAATCATATTTACCAAATGCCCCTCATTTACATAAATATTATACTA GCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCTCAT ATCCTCCCTACTATGCCTAGAAGGAATAATACTATCGCTGTTCATTATAG CTACTCTCATAACCCTCAACACCCACTCCCTCTTAGCCAATATTGTGCCT ATTGCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCT ACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGTACATAACCTAA ACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTACTACCAC TGACATGACTTTCCAAAAAGCACATAATTTGAATCAACACAACCACCCAC AGCCTAATTATTAGCATCATCCCCCTACTATTTTTTAACCAAATCAACAA CAACCTATTTAGCTGTTCCCCAACCTTTTCCTCCGACCCCCTAACAACCC CCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGGCAAGC CAACGCCACTTATCCAGCGAACCACTATCACGAAAAAAACTCTACCTCTC TATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCCACAG AACTAATCATATTTTATATCTTCTTCGAAACCACACTTATCCCCACCTTG GCTATCATCACCCGATGAGGCAACCAGCCAGAACGCCTGAACGCAGGCAC ATACTTCCTATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCATCGCAC TAATTTACACTCACAACACCCTAGGCTCACTAAACATTCTACTACTCACT CTCACTGCCCAAGAACTATCAAACTCCTGAGCCAACAACTTAATATGACT AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT TATGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAATAGTA CTTGCCGCAGTACTCTTAAAACTAGGCGGCTATGGTATAATACGCCTCAC ACTCATTCTCAACCCCCTGACAAAACACATAGCCTACCCCTTCCTTGTAC TATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACA GACCTAAAATCGCTCATTGCATACTCTTCAATCAGCCACATAGCCCTCGT AGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAGTCA TTCTCATAATCGCCCACGGACTCACATCCTCATTACTATTCTGCCTAGCA AACTCAAACTACGAACGCACTCACAGTCGCATCATAATCCTCTCTCAAGG ACTTCAAACTCTACTCCCACTAATAGCTTTTTGATGACTTCTAGCAAGCC TCGCTAACCTCGCCTTACCCCCCACTATTAACCTACTGGGAGAACTCTCT GTGCTAGTAACCACGTTCTCCTGATCAAATATCACTCTCCTACTTACAGG ACTCAACATACTAGTCACAGCCCTATACTCCCTCTACATATTTACCACAA CACAATGGGGCTCACTCACCCACCACATTAACAACATAAAACCCTCATTC ACACGAGAAAACACCCTCATGTTCATACACCTATCCCCCATTCTCCTCCT ATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATAGTT TAACCAAAACATCAGATTGTGAATCTGACAACAGAGGCTTACGACCCCTT ATTTACCGAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAA CAACATGGCTTTCTCAACTTTTAAAGGATAACAGCTATCCATTGGTCTTA GGCCCCAAAAATTTTGGTGCAACTCCAAATAAAAGTAATAACCATGCACA CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC ACCCTCGTTAACCCTAACAAAAAAAACTCATACCCCCATTATGTAAAATC CATTGTCGCATCCACCTTTATTATCAGTCTCTTCCCCACAACAATATTCA TGTGCCTAGACCAAGAAGTTATTATCTCGAACTGACACTGAGCCACAACC CAAACAACCCAGCTCTCCCTAAGCTTCAAACTAGACTACTTCTCCATAAT ATTCATCCCTGTAGCATTGTTCGTTACATGGTCCATCATAGAATTCTCAC TGTGATATATAAACTCAGACCCAAACATTAATCAGTTCTTCAAATATCTA CTCATTTTCCTAATTACCATACTAATCTTAGTTACCGCTAACAACCTATT CCAACTGTTCATCGGCTGAGAGGGCGTAGGAATTATATCCTTCTTGCTCA TCAGTTGATGATACGCCCGAGCAGATGCCAACACAGCAGCCATTCAAGCA GTCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATG ATTTATCCTACACTCCAACTCATGAGACCCACAACAAATAGCCCTTCTAA ACGCTAATCCAAGCCTCACCCCACTACTAGGCCTCCTCCTAGCAGCAGCA GGCAAATCAGCCCAATTAGGTCTCCACCCCTGACTCCCCTCAGCCATAGA AGGCCCCACCCCAGTCTCAGCCCTACTCCACTCAAGCACTATAGTTGTAG CAGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCAGAAAATAGCCCA CTAATCCAAACTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGC AGCAGTCTGCGCCCTTACACAAAATGACATCAAAAAAATCGTAGCCTTCT CCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCATCAACCAA CCACACCTAGCATTCCTGCACATCTGTACCCACGCCTTCTTCAAAGCCAT ACTATTTATGTGCTCCGGGTCCATCATCCACAACCTTAACAATGAACAAG ATATTCGAAAAATAGGAGGACTACTCAAAACCATACCTCTCACTTCAACC TCCCTCACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTCACAGG TTTCTACTCCAAAGACCACATCATCGAAACCGCAAACATATCATACACAA ACGCCTGAGCCCTATCTATTACTCTCATCGCTACCTCCCTGACAAGCGCC TATAGCACTCGAATAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCC CACCCTTACTAACATTAACGAAAATAACCCCACCCTACTAAACCCCATTA AACGCCTGGCAGCCGGAAGCCTATTCGCAGGATTTCTCATTACTAACAAC ATTTCCCCCGCATCCCCCTTCCAAACAACAATCCCCCTCTACCTAAAACT CACAGCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCA ACTACCTAACCAACAAACTTAAAATAAAATCCCCACTATGCACATTTTAT TTCTCCAACATACTCGGATTCTACCCTAGCATCACACACCGCACAATCCC CTATCTAGGCCTTCTTACGAGCCAAAACCTGCCCCTACTCCTCCTAGACC TAACCTGACTAGAAAAGCTATTACCTAAAACAATTTCACAGCACCAAATC TCCACCTCCATCATCACCTCAACCCAAAAAGGCATAATTAAACTTTACTT CCTCTCTTTCTTCTTCCCACTCATCCTAACCCTACTCCTAATCACATAAC CTATTCCCCCGAGCAATCTCAATTACAATATATACACCAACAAACAATGT TCAACCAGTAACCACTACTAATCAACGCCCATAATCATACAAAGCCCCCG CACCAATAGGATCCTCCCGAATCAACCCTGACCCCTCTCCTTCATAAATT ATTCAGCTTCCTACACTATTAAAGTTTACCACAACCACCACCCCATCATA CTCTTTCACCCACAGCACCAATCCTACCTCCATCGCTAACCCCACTAAAA CACTCACCAAGACCTCAACCCCTGACCCCCATGCCTCAGGATACTCCTCA ATAGCCATCGCTGTAGTATATCCAAAGACAACCATCATTCCCCCTAAATA AATTAAAAAAACTATTAAACCCATATAACCTCCCCCAAAATTCAGAATAA TAACACACCCGACCACACCGCTAACAATCAGTACTAAACCCCCATAAATA GGAGAAGGCTTAGAAGAAAACCCCACAAACCCCATTACTAAACCCACACT CAACAGAAACAAAGCATACATCATTATTCTCGCACGGACTACAACCACGA CCAATGATATGAAAAACCATCGTTGTATTTCAACTACAAGAACACCAATG ACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATT CATCGACCTCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCT CACTCCTTGGCGCCTGCCTGATCCTCCAAATCACCACAGGACTATTCCTA GCCATACACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCA CATCACTCGAGACGTAAATTATGGCTGAATCATCCGCTACCTTCACGCCA ATGGCGCCTCAATATTCTTTATCTGCCTCTTCCTACACATCGGGCGAGGC CTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTAT CCTCCTGCTTGCAACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGT GAGGCCAAATATCATTCTGAGGGGCCACAGTAATTACAAACTTACTATCC GCCATCCCATACATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTA CTCAGTAGACAGTCCCACCCTCACACGATTCTTTACCTTTCACTTCATCT TACCCTTCATTATTGCAGCCCTAGCAGCACTCCACCTCCTATTCTTGCAC GAAACGGGATCAAACAACCCCCTAGGAATCACCTCCCATTCCGATAAAAT CACCTTCCACCCTTACTACACAATCAAAGACGCCCTCGGCTTACTTCTCT TCCTTCTCTCCTTAATGACATTAACACTATTCTCACCAGACCTCCTAGGC GACCCAGACAATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACAT CAAGCCCGAATGATATTTCCTATTCGCCTACACAATTCTCCGATCCGTCC CTAACAAACTAGGAGGCGTCCTTGCCCTATTACTATCCATCCTCATCCTA GCAATAATCCCCATCCTCCATATATCCAAACAACAAAGCATAATATTTCG CCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCAGACCTCCTCATTC TAACCTGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGA CAAGTAGCATCCGTACTATACTTCACAACAATCCTAATCCTAATACCAAC TATCTCCCTAATTGAAAACAAAATACTCAAATGGGCCTGTCCTTGTAGTA TAAACTAATACACCAGTCTTGTAAACCGGAGACGAAAACCTTTTTCCAAG GACAAATCAGAGAAAAAGTCTTTAACTCCACCATTAGCACCCAAAGCTAA GATTCTAATTTAAACTATTCTCTGTTCTTTCATGGGGAAGCAGATTTGGG TACCACCCAAGTATTGACTCACCCATCAACAACCGCTATGTATTTCGTAC ATTACTGCCAGCCACCATGAATATTGTACGGTACCATAAATACTTGACCA CCTGTAGTACATAAAAACCCAACCCACATCAAACCCCCCCCCCCCATGCT TACAAGCAAGTACAGCAATCAACCTTCAACTATCACACATCAACTGCAAC TCCAAAGCCACCCCTCACCCACTAGGATACCAACAAACCTACCCACCCTT AACAGTACATAGTACATAAAGTCATTTACCGTACATAGCACATTACAGTC AAATCCCTTCTCGTCCCCATGGATGACCCCCCTCAGATAGGGGTCCCTTG ACCACCATCCTCCGTGAAATCAATATCCCGCACAAGAGTGCTACTCTCCT CGCTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGAC ATCTGGTTCCTACTTCAGGGCCATAAAGCCTAAATAGCCCACACGTTCCC CTTAAATAAGACATCACGATG tantan-22/test/titin_human.fa0000644000000000000000000010436413406427735015113 0ustar rootroot>sp|Q8WZ42|TITIN_HUMAN MTTQAPTFTQPLQSVVVLEGSTATFEAHISGFPVPEVSWFRDGQVISTST LPGVQISFSDGRAKLTIPAVTKANSGRYSLKATNGSGQATSTAELLVKAE TAPPNFVQRLQSMTVRQGSQVRLQVRVTGIPTPVVKFYRDGAEIQSSLDF QISQEGDLYSLLIAEAYPEDSGTYSVNATNSVGRATSTAELLVQGEEEVP AKKTKTIVSTAQISESRQTRIEKKIEAHFDARSIATVEMVIDGAAGQQLP HKTPHRIPPKPKSRSPTPPSIAAKAQLARQQSPSPIRHSPSPVRHVRAPT PSPVRSVSPAARISTSPIRSVRSPLLMRKTQASTVATGPEVPPPWKQEGY VASSSEAEMRETTLTTSTQIRTEERWEGRYGVQEQVTISGAAGAAASVSA SASYAAEAVATGAKEVKQDADKSAAVATVVAAVDMARVREPVISAVEQTA QRTTTTAVHIQPAQEQVRKEAEKTAVTKVVVAADKAKEQELKSRTKEVIT TKQEQMHVTHEQIRKETEKTFVPKVVISAAKAKEQETRISEEITKKQKQV TQEAIRQETEITAASMVVVATAKSTKLETVPGAQEETTTQQDQMHLSYEK IMKETRKTVVPKVIVATPKVKEQDLVSRGREGITTKREQVQITQEKMRKE AEKTALSTIAVATAKAKEQETILRTRETMATRQEQIQVTHGKVDVGKKAE AVATVVAAVDQARVREPREPGHLEESYAQQTTLEYGYKERISAAKVAEPP QRPASEPHVVPKAVKPRVIQAPSETHIKTTDQKGMHISSQIKKTTDLTTE RLVHVDKRPRTASPHFTVSKISVPKTEHGYEASIAGSAIATLQKELSATS SAQKITKSVKAPTVKPSETRVRAEPTPLPQFPFADTPDTYKSEAGVEVKK EVGVSITGTTVREERFEVLHGREAKVTETARVPAPVEIPVTPPTLVSGLK NVTVIEGESVTLECHISGYPSPTVTWYREDYQIESSIDFQITFQSGIARL MIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFEKETTAVTEKFT TEEKRFVESRDVVMTDTSLTEEQAGPGEPAAPYFITKPVVQKLVEGGSVV FGCQVGGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADD AGEYTIVVRNKHGETSASASLLEEADYELLMKSQQEMLYQTQVTAFVQEP KVGETAPGFVYSEYEKEYEKEQALIRKKMAKDTVVVRTYVEDQEFHISSF EERLIKEIEYRIIKTTLEELLEEDGEEKMAVDISESEAVESGFDLRIKNY RILEGMGVTFHCKMSGYPLPKIAWYKDGKRIKHGERYQMDFLQDGRASLR IPVVLPEDEGIYTAFASNIKGNAICSGKLYVEPAAPLGAPTYIPTLEPVS RIRSLSPRSVSRSPIRMSPARMSPARMSPARMSPARMSPGRRLEETDESQ LERLYKPVFVLKPVSFKCLEGQTARFDLKVVGRPMPETFWFHDGQQIVND YTHKVVIKEDGTQSLIIVPATPSDSGEWTVVAQNRAGRSSISVILTVEAV EHQVKPMFVEKLKNVNIKEGSQLEMKVRATGNPNPDIVWLKNSDIIVPHK YPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGRDTTRCKVNVEVE FAEPEPERKLIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQ QKPFFKKKLTSLRLKRFGPAHFECRLTPIGDPTMVVEWLHDGKPLEAANR LRMINEFGYCSLDYGVAYSRDSGIITCRATNKYGTDHTSATLIVKDEKSL VEESQLPEGRKGLQRIEELERMAHEGALTGVTTDQKEKQKPDIVLYPEPV RVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDGIHYLDIV DCKSYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRRAPEPRPEFH VHEPGKLQFEVQKVDRPVDTTETKEVVKLKRAERITHEKVPEESEELRSK FKRRTEEGYYEAITAVELKSRKKDESYEELLRKTKDELLHWTKELTEEEK KALAEEGKITIPTFKPDKIELSPSMEAPKIFERIQSQTVGQGSDAHFRVR VVGKPDPECEWYKNGVKIERSDRIYWYWPEDNVCELVIRDVTAEDSASIM VKAINIAGETSSHAFLLVQAKQLITFTQELQDVVAKEKDTMATFECETSE PFVKVKWYKDGMEVHEGDKYRMHSDRKVHFLSILTIDTSDAEDYSCVLVE DENVKTTAKLIVEGAVVEFVKELQDIEVPESYSGELECIVSPENIEGKWY HNDVELKSNGKYTITSRRGRQNLTVKDVTKEDQGEYSFVIDGKKTTCKLK MKPRPIAILQGLSDQKVCEGDIVQLEVKVSLESVEGVWMKDGQEVQPSDR VHIVIDKQSHMLLIEDMTKEDAGNYSFTIPALGLSTSGRVSVYSVDVITP LKDVNVIEGTKAVLECKVSVPDVTSVKWYLNDEQIKPDDRVQAIVKGTKQ RLVINRTHASDEGPYKLIVGRVETNCNLSVEKIKIIRGLRDLTCTETQNV VFEVELSHSGIDVLWNFKDKEIKPSSKYKIEAHGKIYKLTVLNMMKDDEG KYTFYAGENITSGKLTVAGGAISKPLTDQTVAESQEAVFECEVANPDSKG EWLRDGKHLPLTNNIRSESDGHKRRLIIAATKLDDIGEYTYKVATSKTSA KLKVEAVKIKKTLKNLTVTETQDAVFTVELTHPNVKGVQWIKNGVVLESN EKYAISVKGTIYSLRIKNCAIVDESVYGFRLGRLGASARLHVETVKIIKK PKDVTALENATVAFEVSVSHDTVPVKWFHKNVEIKPSDKHRLVSERKVHK LMLQNISPSDAGEYTAVVGQLECKAKLFVETLHITKTMKNIEVPETKTAS FECEVSHFNVPSMWLKNGVEIEMSEKFKIVVQGKLHQLIIMNTSTEDSAE YTFVCGNDQVSATLTVTPIMITSMLKDINAEEKDTITFEVTVNYEGISYK WLKNGVEIKSTDKCQMRTKKLTHSLNIRNVHFGDAADYTFVAGKATSTAT LYVEARHIEFRKHIKDIKVLEKKRAMFECEVSEPDITVQWMKDDQELQIT DRIKIQKEKYVHRLLIPSTRMSDAGKYTVVAGGNVSTAKLFVEGRDVRIR SIKKEVQVIEKQRAVVEFEVNEDDVDAHWYKDGIEINFQVQERHKYVVER RIHRMFISETRQSDAGEYTFVAGRNRSSVTLYVNAPEPPQVLQELQPVTV QSGKPARFCAVISGRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIE AFPEDAAVYTCEAKNDYGVATTSASLSVEVPEVVSPDQEMPVYPPAIITP LQDTVTSEGQPARFQCRVSGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQ LEIAEAYPEDEGTYTFVASNAVGQVSSTANLSLEAPESILHERIEQEIEM EMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYSTKFDSEKEG TGPIFIKEVSNADISMGDVATLSVTVIGIPKPKIQWFFNGVLLTPSADYK FVFDGDDHSLIILFTKLEDEGEYTCMASNDYGKTICSAYLKINSKGEGHK DTETESAVAKSLEKLGGPCPPHFLKELKPIRCAQGLPAIFEYTVVGEPAP TVTWFKENKQLCTSVYYTIIHNPNGSGTFIVNDPQREDSGLYICKAENML GESTCAAELLVLLEDTDMTDTPCKAKSTPEAPEDFPQTPLKGPAVEALDS EQEIATFVKDTILKAALITEENQQLSYEHIAKANELSSQLPLGAQELQSI LEQDKLTPESTREFLCINGSIHFQPLKEPSPNLQLQIVQSQKTFSKEGIL MPEEPETQAVLSDTEKIFPSAMSIEQINSLTVEPLKTLLAEPEGNYPQSS IEPPMHSYLTSVAEEVLSPKEKTVSDTNREQRVTLQKQEAQSALILSQSL AEGHVESLQSPDVMISQVNYEPLVPSEHSCTEGGKILIESANPLENAGQD SAVRIEEGKSLRFPLALEEKQVLLKEEHSDNVVMPPDQIIESKREPVAIK KVQEVQGRDLLSKESLLSGIPEEQRLNLKIQICRALQAAVASEQPGLFSE WLRNIEKVEVEAVNITQEPRHIMCMYLVTSAKSVTEEVTIIIEDVDPQMA NLKMELRDALCAIIYEEIDILTAEGPRIQQGAKTSLQEEMDSFSGSQKVE PITEPEVESKYLISTEEVSYFNVQSRVKYLDATPVTKGVASAVVSDEKQD ESLKPSEEKEESSSESGTEEVATVKIQEAEGGLIKEDGPMIHTPLVDTVS EEGDIVHLTTSITNAKEVNWYFENKLVPSDEKFKCLQDQNTYTLVIDKVN TEDHQGEYVCEALNDSGKTATSAKLTVVKRAAPVIKRKIEPLEVALGHLA KFTCEIQSAPNVRFQWFKAGREIYESDKCSIRSSKYISSLEILRTQVVDC GEYTCKASNEYGSVSCTATLTVTEAYPPTFLSRPKSLTTFVGKAAKFICT VTGTPVIETIWQKDGAALSPSPNWKISDAENKHILELSNLTIQDRGVYSC KASNKFGADICQAELIIIDKPHFIKELEPVQSAINKKVHLECQVDEDRKV TVTWSKDGQKLPPGKDYKICFEDKIATLEIPLAKLKDSGTYVCTASNEAG SSSCSATVTVREPPSFVKKVDPSYLMLPGESARLHCKLKGSPVIQVTWFK NNKELSESNTVRMYFVNSEAILDITDVKVEDSGSYSCEAVNDVGSDSCST EIVIKEPPSFIKTLEPADIVRGTNALLQCEVSGTGPFEISWFKDKKQIRS SKKYRLFSQKSLVCLEIFSFNSADVGEYECVVANEVGKCGCMATHLLKEP PTFVKKVDDLIALGGQTVTLQAAVRGSEPISVTWMKGQEVIREDGKIKMS FSNGVAVLIIPDVQISFGGKYTCLAENEAGSQTSVGELIVKEPAKIIERA ELIQVTAGDPATLEYTVAGTPELKPKWYKDGRPLVASKKYRISFKNNVAQ LKFYSAELHDSGQYTFEISNEVGSSSCETTFTVLDRDIAPFFTKPLRNVD SVVNGTCRLDCKIAGSLPMRVSWFKDGKEIAASDRYRIAFVEGTASLEII RVDMNDAGNFTCRATNSVGSKDSSGALIVQEPPSFVTKPGSKDVLPGSAV CLKSTFQGSTPLTIRWFKGNKELVSGGSCYITKEALESSLELYLVKTSDS GTYTCKVSNVAGGVECSANLFVKEPATFVEKLEPSQLLKKGDATQLACKV TGTPPIKITWFANDREIKESSKHRMSFVESTAVLRLTDVGIEDSGEYMCE AQNEAGSDHCSSIVIVKESPYFTKEFKPIEVLKEYDVMLLAEVAGTPPFE ITWFKDNTILRSGRKYKTFIQDHLVSLQILKFVAADAGEYQCRVTNEVGS SICSARVTLREPPSFIKKIESTSSLRGGTAAFQATLKGSLPITVTWLKDS DEITEDDNIRMTFENNVASLYLSGIEVKHDGKYVCQAKNDAGIQRCSALL SVKEPATITEEAVSIDVTQGDPATLQVKFSGTKEITAKWFKDGQELTLGS KYKISVTDTVSILKIISTEKKDSGEYTFEVQNDVGRSSCKARINVLDLII PPSFTKKLKKMDSIKGSFIDLECIVAGSHPISIQWFKDDQEISASEKYKF SFHDNTAFLEISQLEGTDSGTYTCSATNKAGHNQCSGHLTVKEPPYFVEK PQSQDVNPNTRVQLKALVGGTAPMTIKWFKDNKELHSGAARSVWKDDTST SLELFAAKATDSGTYICQLSNDVGTATSKATLFVKEPPQFIKKPSPVLVL RNGQSTTFECQITGTPKIRVSWYLDGNEITAIQKHGISFIDGLATFQISG ARVENSGTYVCEARNDAGTASCSIELKVKEPPTFIRELKPVEVVKYSDVE LECEVTGTPPFEVTWLKNNREIRSSKKYTLTDRVSVFNLHITKCDPSDTG EYQCIVSNEGGSCSCSTRVALKEPPSFIKKIENTTTVLKSSATFQSTVAG SPPISITWLKDDQILDEDDNVYISFVDSVATLQIRSVDNGHSGRYTCQAK NESGVERCYAFLLVQEPAQIVEKAKSVDVTEKDPMTLECVVAGTPELKVK WLKDGKQIVPSRYFSMSFENNVASFRIQSVMKQDSGQYTFKVENDFGSSS CDAYLRVLDQNIPPSFTKKLTKMDKVLGSSIHMECKVSGSLPISAQWFKD GKEISTSAKYRLVCHERSVSLEVNNLELEDTANYTCKVSNVAGDDACSGI LTVKEPPSFLVKPGRQQAIPDSTVEFKAILKGTPPFKIKWFKDDVELVSG PKCFIGLEGSTSFLNLYSVDASKTGQYTCHVTNDVGSDSCTTMLLVTEPP KFVKKLEASKIVKAGDSSRLECKIAGSPEIRVVWFRNEHELPASDKYRMT FIDSVAVIQMNNLSTEDSGDFICEAQNPAGSTSCSTKVIVKEPPVFSSFP PIVETLKNAEVSLECELSGTPPFEVVWYKDKRQLRSSKKYKIASKNFHTS IHILNVDTSDIGEYHCKAQNEVGSDTCVCTVKLKEPPRFVSKLNSLTVVA GEPAELQASIEGAQPIFVQWLKEKEEVIRESENIRITFVENVATLQFAKA EPANAGKYICQIKNDGGMEENMATLMVLEPAVIVEKAGPMTVTVGETCTL ECKVAGTPELSVEWYKDGKLLTSSQKHKFSFYNKISSLRILSVERQDAGT YTFQVQNNVGKSSCTAVVDVSDRAVPPSFTRRLKNTGGVLGASCILECKV AGSSPISVAWFHEKTKIVSGAKYQTTFSDNVCTLQLNSLDSSDMGNYTCV AANVAGSDECRAVLTVQEPPSFVKEPEPLEVLPGKNVTFTSVIRGTPPFK VNWFRGARELVKGDRCNIYFEDTVAELELFNIDISQSGEYTCVVSNNAGQ ASCTTRLFVKEPAAFLKRLSDHSVEPGKSIILESTYTGTLPISVTWKKDG FNITTSEKCNIVTTEKTCILEILNSTKRDAGQYSCEIENEAGRDVCGALV STLEPPYFVTELEPLEAAVGDSVSLQCQVAGTPEITVSWYKGDTKLRPTP EYRTYFTNNVATLVFNKVNINDSGEYTCKAENSIGTASSKTVFRIQERQL PPSFARQLKDIEQTVGLPVTLTCRLNGSAPIQVCWYRDGVLLRDDENLQT SFVDNVATLKILQTDLSHSGQYSCSASNPLGTASSSARLTAREPKKSPFF DIKPVSIDVIAGESADFECHVTGAQPMRITWSKDNKEIRPGGNYTITCVG NTPHLRILKVGKGDSGQYTCQATNDVGKDMCSAQLSVKEPPKFVKKLEAS KVAKQGESIQLECKISGSPEIKVSWFRNDSELHESWKYNMSFINSVALLT INEASAEDSGDYICEAHNGVGDASCSTALTVKAPPVFTQKPSPVGALKGS DVILQCEISGTPPFEVVWVKDRKQVRNSKKFKITSKHFDTSLHILNLEAS DVGEYHCKATNEVGSDTCSCSVKFKEPPRFVKKLSDTSTLIGDAVELRAI VEGFQPISVVWLKDRGEVIRESENTRISFIDNIATLQLGSPEASNSGKYI CQIKNDAGMRECSAVLTVLEPARIIEKPEPMTVTTGNPFALECVVTGTPE LSAKWFKDGRELSADSKHHITFINKVASLKIPCAEMSDKGLYSFEVKNSV GKSNCTVSVHVSDRIVPPSFIRKLKDVNAILGASVVLECRVSGSAPISVG WFQDGNEIVSGPKCQSSFSENVCTLNLSLLEPSDTGIYTCVAANVAGSDE CSAVLTVQEPPSFEQTPDSVEVLPGMSLTFTSVIRGTPPFKVKWFKGSRE LVPGESCNISLEDFVTELELFEVQPLESGDYSCLVTNDAGSASCTTHLFV KEPATFVKRLADFSVETGSPIVLEATYTGTPPISVSWIKDEYLISQSERC SITMTEKSTILEILESTIEDYAQYSCLIENEAGQDICEALVSVLEPPYFI EPLEHVEAVIGEPATLQCKVDGTPEIRISWYKEHTKLRSAPAYKMQFKNN VASLVINKVDHSDVGEYSCKADNSVGAVASSAVLVIKERKLPPFFARKLK DVHETLGFPVAFECRINGSEPLQVSWYKDGVLLKDDANLQTSFVHNVATL QILQTDQSHIGQYNCSASNPLGTASSSAKLILSEHEVPPFFDLKPVSVDL ALGESGTFKCHVTGTAPIKITWAKDNREIRPGGNYKMTLVENTATLTVLK VGKGDAGQYTCYASNIAGKDSCSAHLGVQEPPRFIKKLEPSRIVKQDEFT RYECKIGGSPEIKVLWYKDETEIQESSKFRMSFVDSVAVLEMHNLSVEDS GDYTCEAHNAAGSASSSTSLKVKEPPIFRKKPHPIETLKGADVHLECELQ GTPPFHVSWYKDKRELRSGKKYKIMSENFLTSIHILNVDAADIGEYQCKA TNDVGSDTCVGSIALKAPPRFVKKLSDISTVVGKEVQLQTTIEGAEPISV VWFKDKGEIVRESDNIWISYSENIATLQFSRVEPANAGKYTCQIKNDAGM QECFATLSVLEPATIVEKPESIKVTTGDTCTLECTVAGTPELSTKWFKDG KELTSDNKYKISFFNKVSGLKIINVAPSDSGVYSFEVQNPVGKDSCTASL QVSDRTVPPSFTRKLKETNGLSGSSVVMECKVYGSPPISVSWFHEGNEIS SGRKYQTTLTDNTCALTVNMLEESDSGDYTCIATNMAGSDECSAPLTVRE PPSFVQKPDPMDVLTGTNVTFTSIVKGTPPFSVSWFKGSSELVPGDRCNV SLEDSVAELELFDVDTSQSGEYTCIVSNEAGKASCTTHLYIKAPAKFVKR LNDYSIEKGKPLILEGTFTGTPPISVTWKKNGINVTPSQRCNITTTEKSA ILEIPSSTVEDAGQYNCYIENASGKDSCSAQILILEPPYFVKQLEPVKVS VGDSASLQCQLAGTPEIGVSWYKGDTKLRPTTTYKMHFRNNVATLVFNQV DINDSGEYICKAENSVGEVSASTFLTVQEQKLPPSFSRQLRDVQETVGLP VVFDCAISGSEPISVSWYKDGKPLKDSPNVQTSFLDNTATLNIFKTDRSL AGQYSCTATNPIGSASSSARLILTEGKNPPFFDIRLAPVDAVVGESADFE CHVTGTQPIKVSWAKDSREIRSGGKYQISYLENSAHLTVLKVDKGDSGQY TCYAVNEVGKDSCTAQLNIKERLIPPSFTKRLSETVEETEGNSFKLEGRV AGSQPITVAWYKNNIEIQPTSNCEITFKNNTLVLQVRKAGMNDAGLYTCK VSNDAGSALCTSSIVIKEPKKPPVFDQHLTPVTVSEGEYVQLSCHVQGSE PIRIQWLKAGREIKPSDRCSFSFASGTAVLELRDVAKADSGDYVCKASNV AGSDTTKSKVTIKDKPAVAPATKKAAVDGRLFFVSEPQSIRVVEKTTATF IAKVGGDPIPNVKWTKGKWRQLNQGGRVFIHQKGDEAKLEIRDTTKTDSG LYRCVAFNEHGEIESNVNLQVDERKKQEKIEGDLRAMLKKTPILKKGAGE EEEIDIMELLKNVDPKEYEKYARMYGITDFRGLLQAFELLKQSQEEETHR LEIEEIERSERDEKEFEELVSFIQQRLSQTEPVTLIKDIENQTVLKDNDA VFEIDIKINYPEIKLSWYKGTEKLEPSDKFEISIDGDRHTLRVKNCQLKD QGNYRLVCGPHIASAKLTVIEPAWERHLQDVTLKEGQTCTMTCQFSVPNV KSEWFRNGRILKPQGRHKTEVEHKVHKLTIADVRAEDQGQYTCKYEDLET SAELRIEAEPIQFTKRIQNIVVSEHQSATFECEVSFDDAIVTWYKGPTEL TESQKYNFRNDGRCHYMTIHNVTPDDEGVYSVIARLEPRGEARSTAELYL TTKEIKLELKPPDIPDSRVPIPTMPIRAVPPEEIPPVVAPPIPLLLPTPE EKKPPPKRIEVTKKAVKKDAKKVVAKPKEMTPREEIVKKPPPPTTLIPAK APEIIDVSSKAEEVKIMTITRKKEVQKEKEAVYEKKQAVHKEKRVFIESF EEPYDELEVEPYTEPFEQPYYEEPDEDYEEIKVEAKKEVHEEWEEDFEEG QEYYEREEGYDEGEEEWEEAYQEREVIQVQKEVYEESHERKVPAKVPEKK APPPPKVIKKPVIEKIEKTSRRMEEEKVQVTKVPEVSKKIVPQKPSRTPV QEEVIEVKVPAVHTKKMVISEEKMFFASHTEEEVSVTVPEVQKEIVTEEK IHVAVSKRVEPPPKVPELPEKPAPEEVAPVPIPKKVEPPAPKVPEVPKKP VPEEKKPVPVPKKEPAAPPKVPEVPKKPVPEEKIPVPVAKKKEAPPAKVP EVQKRVVTEEKITIVTQREESPPPAVPEIPKKKVPEERKPVPRKEEEVPP PPKVPALPKKPVPEEKVAVPVPVAKKAPPPRAEVSKKTVVEEKRFVAEEK LSFAVPQRVEVTRHEVSAEEEWSYSEEEEGVSISVYREEEREEEEEAEVT EYEVMEEPEEYVVEEKLHIISKRVEAEPAEVTERQEKKIVLKPKIPAKIE EPPPAKVPEAPKKIVPEKKVPAPVPKKEKVPPPKVPEEPKKPVPEKKVPP KVIKMEEPLPAKVTERHMQITQEEKVLVAVTKKEAPPKARVPEEPKRAVP EEKVLKLKPKREEEPPAKVTEFRKRVVKEEKVSIEAPKREPQPIKEVTIM EEKERAYTLEEEAVSVQREEEYEEYEEYDYKEFEEYEPTEEYDQYEEYEE REYERYEEHEEYITEPEKPIPVKPVPEEPVPTKPKAPPAKVLKKAVPEEK VPVPIPKKLKPPPPKVPEEPKKVFEEKIRISITKREKEQVTEPAAKVPMK PKRVVAEEKVPVPRKEVAPPVRVPEVPKELEPEEVAFEEEVVTHVEEYLV EEEEEYIHEEEEFITEEEVVPVIPVKVPEVPRKPVPEEKKPVPVPKKKEA PPAKVPEVPKKPEEKVPVLIPKKEKPPPAKVPEVPKKPVPEEKVPVPVPK KVEAPPAKVPEVPKKPVPEKKVPVPAPKKVEAPPAKVPEVPKKLIPEEKK PTPVPKKVEAPPPKVPKKREPVPVPVALPQEEEVLFEEEIVPEEEVLPEE EEVLPEEEEVLPEEEEVLPEEEEIPPEEEEVPPEEEYVPEEEEFVPEEEV LPEVKPKVPVPAPVPEIKKKVTEKKVVIPKKEEAPPAKVPEVPKKVEEKR IILPKEEEVLPVEVTEEPEEEPISEEEIPEEPPSIEEVEEVAPPRVPEVI KKAVPEAPTPVPKKVEAPPAKVSKKIPEEKVPVPVQKKEAPPAKVPEVPK KVPEKKVLVPKKEAVPPAKGRTVLEEKVSVAFRQEVVVKERLELEVVEAE VEEIPEEEEFHEVEEYFEEGEFHEVEEFIKLEQHRVEEEHRVEKVHRVIE VFEAEEVEVFEKPKAPPKGPEISEKIIPPKKPPTKVVPRKEPPAKVPEVP KKIVVEEKVRVPEEPRVPPTKVPDVLPPKEVVPEKKVPVPPAKKPEAPPP KVPEAPKEVVPEKKVPVPPPKKPEVPPTKVPEVPKAAVPEKKVPEAIPPK PESPPPEVPEAPKEVVPEKKVPAAPPKKPEVTPVKVPEAPKEVVPEKKVP VPPPKKPEVPPTKVPEVPKVAVPEKKVPEAIPPKPESPPPEVFEEPEEVA LEEPPAEVVEEPEPAAPPQVTVPPKKPVPEKKAPAVVAKKPELPPVKVPE VPKEVVPEKKVPLVVPKKPEAPPAKVPEVPKEVVPEKKVAVPKKPEVPPA KVPEVPKKPVLEEKPAVPVPERAESPPPEVYEEPEEIAPEEEIAPEEEKP VPVAEEEEPEVPPPAVPEEPKKIIPEKKVPVIKKPEAPPPKEPEPEKVIE KPKLKPRPPPPPPAPPKEDVKEKIFQLKAIPKKKVPEKPQVPEKVELTPL KVPGGEKKVRKLLPERKPEPKEEVVLKSVLRKRPEEEEPKVEPKKLEKVK KPAVPEPPPPKPVEEVEVPTVTKRERKIPEPTKVPEIKPAIPLPAPEPKP KPEAEVKTIKPPPVEPEPTPIAAPVTVPVVGKKAEAKAPKEEAAKPKGPI KGVPKKTPSPIEAERRKLRPGSGGEKPPDEAPFTYQLKAVPLKFVKEIKD IILTESEFVGSSAIFECLVSPSTAITTWMKDGSNIRESPKHRFIADGKDR KLHIIDVQLSDAGEYTCVLRLGNKEKTSTAKLVVEELPVRFVKTLEEEVT VVKGQPLYLSCELNKERDVVWRKDGKIVVEKPGRIVPGVIGLMRALTIND ADDTDAGTYTVTVENANNLECSSCVKVVEVIRDWLVKPIRDQHVKPKGTA IFACDIAKDTPNIKWFKGYDEIPAEPNDKTEILRDGNHLYLKIKNAMPED IAEYAVEIEGKRYPAKLTLGEREVELLKPIEDVTIYEKESASFDAEISEA DIPGQWKLKGELLRPSPTCEIKAEGGKRFLTLRKVKLDQAGEVLYQALNA ITTAILTVKEIELDFAVPLKDVTVPERRQARFECVLTREANVIWSKGPDI IKSSDKFDIIADGKKHILVINDSQFDDEGVYTAEVEGKKTSARLFVTGIR LKFMSPLEDQTVKEGETATFVCELSHEKMHVVWFKNDAKLHTSRTVLISS EGKTHKLEMKEVTLDDISQIKAQVKELSSTAQLKVLEADPYFTVKLHDKT AVEKDEITLKCEVSKDVPVKWFKDGEEIVPSPKYSIKADGLRRILKIKKA DLKDKGEYVCDCGTDKTKANVTVEARLIKVEKPLYGVEVFVGETAHFEIE LSEPDVHGQWKLKGQPLTASPDCEIIEDGKKHILILHNCQLGMTGEVSFQ AANAKSAANLKVKELPLIFITPLSDVKVFEKDEAKFECEVSREPKTFRWL KGTQEITGDDRFELIKDGTKHSMVIKSAAFEDEAKYMFEAEDKHTSGKLI IEGIRLKFLTPLKDVTAKEKESAVFTVELSHDNIRVKWFKNDQRLHTTRS VSMQDEGKTHSITFKDLSIDDTSQIRVEAMGMSSEAKLTVLEGDPYFTGK LQDYTGVEKDEVILQCEISKADAPVKWFKDGKEIKPSKNAVIKADGKKRM LILKKALKSDIGQYTCDCGTDKTSGKLDIEDREIKLVRPLHSVEVMETET ARFETEISEDDIHANWKLKGEALLQTPDCEIKEEGKIHSLVLHNCRLDQT GGVDFQAANVKSSAHLRVKPRVIGLLRPLKDVTVTAGETATFDCELSYED IPVEWYLKGKKLEPSDKVVPRSEGKVHTLTLRDVKLEDAGEVQLTAKDFK THANLFVKEPPVEFTKPLEDQTVEEGATAVLECEVSRENAKVKWFKNGTE ILKSKKYEIVADGRVRKLVIHDCTPEDIKTYTCDAKDFKTSCNLNVVPPH VEFLRPLTDLQVREKEMARFECELSRENAKVKWFKDGAEIKKGKKYDIIS KGAVRILVINKCLLDDEAEYSCEVRTARTSGMLTVLEEEAVFTKNLANIE VSETDTIKLVCEVSKPGAEVIWYKGDEEIIETGRYEILTEGRKRILVIQN AHLEDAGNYNCRLPSSRTDGKVKVHELAAEFISKPQNLEILEGEKAEFVC SISKESFPVQWKRDDKTLESGDKYDVIADGKKRVLVVKDATLQDMGTYVV MVGAARAAAHLTVIEKLRIVVPLKDTRVKEQQEVVFNCEVNTEGAKAKWF RNEEAIFDSSKYIILQKDLVYTLRIRDAHLDDQANYNVSLTNHRGENVKS AANLIVEEEDLRIVEPLKDIETMEKKSVTFWCKVNRLNVTLKWTKNGEEV PFDNRVSYRVDKYKHMLTIKDCGFPDEGEYIVTAGQDKSVAELLIIEAPT EFVEHLEDQTVTEFDDAVFSCQLSREKANVKWYRNGREIKEGKKYKFEKD GSIHRLIIKDCRLDDECEYACGVEDRKSRARLFVEEIPVEIIRPPQDILE APGADVVFLAELNKDKVEVQWLRNNMVVVQGDKHQMMSEGKIHRLQICDI KPRDQGEYRFIAKDKEARAKLELAAAPKIKTADQDLVVDVGKPLTMVVPY DAYPKAEAEWFKENEPLSTKTIDTTAEQTSFRILEAKKGDKGRYKIVLQN KHGKAEGFINLKVIDVPGPVRNLEVTETFDGEVSLAWEEPLTDGGSKIIG YVVERRDIKRKTWVLATDRAESCEFTVTGLQKGGVEYLFRVSARNRVGTG EPVETDNPVEARSKYDVPGPPLNVTITDVNRFGVSLTWEPPEYDGGAEIT NYVIELRDKTSIRWDTAMTVRAEDLSATVTDVVEGQEYSFRVRAQNRIGV GKPSAATPFVKVADPIERPSPPVNLTSSDQTQSSVQLKWEPPLKDGGSPI LGYIIERCEEGKDNWIRCNMKLVPELTYKVTGLEKGNKYLYRVSAENKAG VSDPSEILGPLTADDAFVEPTMDLSAFKDGLEVIVPNPITILVPSTGYPR PTATWCFGDKVLETGDRVKMKTLSAYAELVISPSERSDKGIYTLKLENRV KTISGEIDVNVIARPSAPKELKFGDITKDSVHLTWEPPDDDGGSPLTGYV VEKREVSRKTWTKVMDFVTDLEFTVPDLVQGKEYLFKVCARNKCGPGEPA YVDEPVNMSTPATVPDPPENVKWRDRTANSIFLTWDPPKNDGGSRIKGYI VERCPRGSDKWVACGEPVAETKMEVTGLEEGKWYAYRVKALNRQGASKPS RPTEEIQAVDTQEAPEIFLDVKLLAGLTVKAGTKIELPATVTGKPEPKIT WTKADMILKQDKRITIENVPKKSTVTIVDSKRSDTGTYIIEAVNVCGRAT AVVEVNVLDKPGPPAAFDITDVTNESCLLTWNPPRDDGGSKITNYVVERR ATDSEVWHKLSSTVKDTNFKATKLIPNKEYIFRVAAENMYGVGEPVQASP ITAKYQFDPPGPPTRLEPSDITKDAVTLTWCEPDDDGGSPITGYWVERLD PDTDKWVRCNKMPVKDTTYRVKGLTNKKKYRFRVLAENLAGPGKPSKSTE PILIKDPIDPPWPPGKPTVKDVGKTSVRLNWTKPEHDGGAKIESYVIEML KTGTDEWVRVAEGVPTTQHLLPGLMEGQEYSFRVRAVNKAGESEPSEPSD PVLCREKLYPPSPPRWLEVINITKNTADLKWTVPEKDGGSPITNYIVEKR DVRRKGWQTVDTTVKDTKCTVTPLTEGSLYVFRVAAENAIGQSDYTEIED SVLAKDTFTTPGPPYALAVVDVTKRHVDLKWEPPKNDGGRPIQRYVIEKK ERLGTRWVKAGKTAGPDCNFRVTDVIEGTEVQFQVRAENEAGVGHPSEPT EILSIEDPTSPPSPPLDLHVTDAGRKHIAIAWKPPEKNGGSPIIGYHVEM CPVGTEKWMRVNSRPIKDLKFKVEEGVVPDKEYVLRVRAVNAIGVSEPSE ISENVVAKDPDCKPTIDLETHDIIVIEGEKLSIPVPFRAVPVPTVSWHKD GKEVKASDRLTMKNDHISAHLEVPKSVRADAGIYTITLENKLGSATASIN VKVIGLPGPCKDIKASDITKSSCKLTWEPPEFDGGTPILHYVLERREAGR RTYIPVMSGENKLSWTVKDLIPNGEYFFRVKAVNKVGGGEYIELKNPVIA QDPKQPPDPPVDVEVHNPTAEAMTITWKPPLYDGGSKIMGYIIEKIAKGE ERWKRCNEHLVPILTYTAKGLEEGKEYQFRVRAENAAGISEPSRATPPTK AVDPIDAPKVILRTSLEVKRGDEIALDASISGSPYPTITWIKDENVIVPE EIKKRAAPLVRRRKGEVQEEEPFVLPLTQRLSIDNSKKGESQLRVRDSLR PDHGLYMIKVENDHGIAKAPCTVSVLDTPGPPINFVFEDIRKTSVLCKWE PPLDDGGSEIINYTLEKKDKTKPDSEWIVVTSTLRHCKYSVTKLIEGKEY LFRVRAENRFGPGPPCVSKPLVAKDPFGPPDAPDKPIVEDVTSNSMLVKW NEPKDNGSPILGYWLEKREVNSTHWSRVNKSLLNALKANVDGLLEGLTYV FRVCAENAAGPGKFSPPSDPKTAHDPISPPGPPIPRVTDTSSTTIELEWE PPAFNGGGEIVGYFVDKQLVGTNEWSRCTEKMIKVRQYTVKEIREGADYK LRVSAVNAAGEGPPGETQPVTVAEPQEPPAVELDVSVKGGIQIMAGKTLR IPAVVTGRPVPTKVWTKEEGELDKDRVVIDNVGTKSELIIKDALRKDHGR YVITATNSCGSKFAAARVEVFDVPGPVLDLKPVVTNRKMCLLNWSDPEDD GGSEITGFIIERKDAKMHTWRQPIETERSKCDITGLLEGQEYKFRVIAKN KFGCGPPVEIGPILAVDPLGPPTSPERLTYTERTKSTITLDWKEPRSNGG SPIQGYIIEKRRHDKPDFERVNKRLCPTTSFLVENLDEHQMYEFRVKAVN EIGESEPSLPLNVVIQDDEVPPTIKLRLSVRGDTIKVKAGEPVHIPADVT GLPMPKIEWSKNETVIEKPTDALQITKEEVSRSEAKTELSIPKAVREDKG TYTVTASNRLGSVFRNVHVEVYDRPSPPRNLAVTDIKAESCYLTWDAPLD NGGSEITHYVIDKRDASRKKAEWEEVTNTAVEKRYGIWKLIPNGQYEFRV RAVNKYGISDECKSDKVVIQDPYRLPGPPGKPKVLARTKGSMLVSWTPPL DNGGSPITGYWLEKREEGSPYWSRVSRAPITKVGLKGVEFNVPRLLEGVK YQFRAMAINAAGIGPPSEPSDPEVAGDPIFPPGPPSCPEVKDKTKSSISL GWKPPAKDGGSPIKGYIVEMQEEGTTDWKRVNEPDKLITTCECVVPNLKE LRKYRFRVKAVNEAGESEPSDTTGEIPATDIQEEPEVFIDIGAQDCLVCK AGSQIRIPAVIKGRPTPKSSWEFDGKAKKAMKDGVHDIPEDAQLETAENS SVIIIPECKRSHTGKYSITAKNKAGQKTANCRVKVMDVPGPPKDLKVSDI TRGSCRLSWKMPDDDGGDRIKGYVIEKRTIDGKAWTKVNPDCGSTTFVVP DLLSEQQYFFRVRAENRFGIGPPVETIQRTTARDPIYPPDPPIKLKIGLI TKNTVHLSWKPPKNDGGSPVTHYIVECLAWDPTGTKKEAWRQCNKRDVEE LQFTVEDLVEGGEYEFRVKAVNAAGVSKPSATVGPCDCQRPDMPPSIDLK EFMEVEEGTNVNIVAKIKGVPFPTLTWFKAPPKKPDNKEPVLYDTHVNKL VVDDTCTLVIPQSRRSDTGLYTITAVNNLGTASKEMRLNVLGRPGPPVGP IKFESVSADQMTLSWFPPKDDGGSKITNYVIEKREANRKTWVHVSSEPKE CTYTIPKLLEGHEYVFRIMAQNKYGIGEPLDSEPETARNLFSVPGAPDKP TVSSVTRNSMTVNWEEPEYDGGSPVTGYWLEMKDTTSKRWKRVNRDPIKA MTLGVSYKVTGLIEGSDYQFRVYAINAAGVGPASLPSDPATARDPIAPPG PPFPKVTDWTKSSADLEWSPPLKDGGSKVTGYIVEYKEEGKEEWEKGKDK EVRGTKLVVTGLKEGAFYKFRVSAVNIAGIGEPGEVTDVIEMKDRLVSPD LQLDASVRDRIVVHAGGVIRIIAYVSGKPPPTVTWNMNERTLPQEATIET TAISSSMVIKNCQRSHQGVYSLLAKNEAGERKKTIIVDVLDVPGPVGTPF LAHNLTNESCKLTWFSPEDDGGSPITNYVIEKRESDRRAWTPVTYTVTRQ NATVQGLIQGKAYFFRIAAENSIGMGPFVETSEALVIREPITVPERPEDL EVKEVTKNTVTLTWNPPKYDGGSEIINYVLESRLIGTEKFHKVTNDNLLS RKYTVKGLKEGDTYEYRVSAVNIVGQGKPSFCTKPITCKDELAPPTLHLD FRDKLTIRVGEAFALTGRYSGKPKPKVSWFKDEADVLEDDRTHIKTTPAT LALEKIKAKRSDSGKYCVVVENSTGSRKGFCQVNVVDRPGPPVGPVSFDE VTKDYMVISWKPPLDDGGSKITNYIIEKKEVGKDVWMPVTSASAKTTCKV SKLLEGKDYIFRIHAENLYGISDPLVSDSMKAKDRFRVPDAPDQPIVTEV TKDSALVTWNKPHDGGKPITNYILEKRETMSKRWARVTKDPIHPYTKFRV PDLLEGCQYEFRVSAENEIGIGDPSPPSKPVFAKDPIAKPSPPVNPEAID TTCNSVDLTWQPPRHDGGSKILGYIVEYQKVGDEEWRRANHTPESCPETK YKVTGLRDGQTYKFRVLAVNAAGESDPAHVPEPVLVKDRLEPPELILDAN MAREQHIKVGDTLRLSAIIKGVPFPKVTWKKEDRDAPTKARIDVTPVGSK LEIRNAAHEDGGIYSLTVENPAGSKTVSVKVLVLDKPGPPRDLEVSEIRK DSCYLTWKEPLDDGGSVITNYVVERRDVASAQWSPLSATSKKKSHFAKHL NEGNQYLFRVAAENQYGRGPFVETPKPIKALDPLHPPGPPKDLHHVDVDK TEVSLVWNKPDRDGGSPITGYLVEYQEEGTQDWIKFKTVTNLECVVTGLQ QGKTYRFRVKAENIVGLGLPDTTIPIECQEKLVPPSVELDVKLIEGLVVK AGTTVRFPAIIRGVPVPTAKWTTDGSEIKTDEHYTVETDNFSSVLTIKNC LRRDTGEYQITVSNAAGSKTVAVHLTVLDVPGPPTGPINILDVTPEHMTI SWQPPKDDGGSPVINYIVEKQDTRKDTWGVVSSGSSKTKLKIPHLQKGCE YVFRVRAENKIGVGPPLDSTPTVAKHKFSPPSPPGKPVVTDITENAATVS WTLPKSDGGSPITGYYMERREVTGKWVRVNKTPIADLKFRVTGLYEGNTY EFRVFAENLAGLSKPSPSSDPIKACRPIKPPGPPINPKLKDKSRETADLV WTKPLSDGGSPILGYVVECQKPGTAQWNRINKDELIRQCAFRVPGLIEGN EYRFRIKAANIVGEGEPRELAESVIAKDILHPPEVELDVTCRDVITVRVG QTIRILARVKGRPEPDITWTKEGKVLVREKRVDLIQDLPRVELQIKEAVR ADHGKYIISAKNSSGHAQGSAIVNVLDRPGPCQNLKVTNVTKENCTISWE NPLDNGGSEITNFIVEYRKPNQKGWSIVASDVTKRLIKANLLANNEYYFR VCAENKVGVGPTIETKTPILAINPIDRPGEPENLHIADKGKTFVYLKWRR PDYDGGSPNLSYHVERRLKGSDDWERVHKGSIKETHYMVDRCVENQIYEF RVQTKNEGGESDWVKTEEVVVKEDLQKPVLDLKLSGVLTVKAGDTIRLEA GVRGKPFPEVAWTKDKDATDLTRSPRVKIDTRADSSKFSLTKAKRSDGGK YVVTATNTAGSFVAYATVNVLDKPGPVRNLKIVDVSSDRCTVCWDPPEDD GGCEIQNYILEKCETKRMVWSTYSATVLTPGTTVTRLIEGNEYIFRVRAE NKIGTGPPTESKPVIAKTKYDKPGRPDPPEVTKVSKEEMTVVWNPPEYDG GKSITGYFLEKKEKHSTRWVPVNKSAIPERRMKVQNLLPDHEYQFRVKAE NEIGIGEPSLPSRPVVAKDPIEPPGPPTNFRVVDTTKHSITLGWGKPVYD GGAPIIGYVVEMRPKIADASPDEGWKRCNAAAQLVRKEFTVTSLDENQEY EFRVCAQNQVGIGRPAELKEAIKPKEILEPPEIDLDASMRKLVIVRAGCP IRLFAIVRGRPAPKVTWRKVGIDNVVRKGQVDLVDTMAFLVIPNSTRDDS GKYSLTLVNPAGEKAVFVNVRVLDTPGPVSDLKVSDVTKTSCHVSWAPPE NDGGSQVTHYIVEKREADRKTWSTVTPEVKKTSFHVTNLVPGNEYYFRVT AVNEYGPGVPTDVPKPVLASDPLSEPDPPRKLEVTEMTKNSATLAWLPPL RDGGAKIDGYITSYREEEQPADRWTEYSVVKDLSLVVTGLKEGKKYKFRV AARNAVGVSLPREAEGVYEAKEQLLPPKILMPEQITIKAGKKLRIEAHVY GKPHPTCKWKKGEDEVVTSSHLAVHKADSSSILIIKDVTRKDSGYYSLTA ENSSGTDTQKIKVVVMDAPGPPQPPFDISDIDADACSLSWHIPLEDGGSN ITNYIVEKCDVSRGDWVTALASVTKTSCRVGKLIPGQEYIFRVRAENRFG ISEPLTSPKMVAQFPFGVPSEPKNARVTKVNKDCIFVAWDRPDSDGGSPI IGYLIERKERNSLLWVKANDTLVRSTEYPCAGLVEGLEYSFRIYALNKAG SSPPSKPTEYVTARMPVDPPGKPEVIDVTKSTVSLIWARPKHDGGSKIIG YFVEACKLPGDKWVRCNTAPHQIPQEEYTATGLEEKAQYQFRAIARTAVN ISPPSEPSDPVTILAENVPPRIDLSVAMKSLLTVKAGTNVCLDATVFGKP MPTVSWKKDGTLLKPAEGIKMAMQRNLCTLELFSVNRKDSGDYTITAENS SGSKSATIKLKVLDKPGPPASVKINKMYSDRAMLSWEPPLEDGGSEITNY IVDKRETSRPNWAQVSATVPITSCSVEKLIEGHEYQFRICAENKYGVGDP VFTEPAIAKNPYDPPGRCDPPVISNITKDHMTVSWKPPADDGGSPITGYL LEKRETQAVNWTKVNRKPIIERTLKATGLQEGTEYEFRVTAINKAGPGKP SDASKAAYARDPQYPPAPPAFPKVYDTTRSSVSLSWGKPAYDGGSPIIGY LVEVKRADSDNWVRCNLPQNLQKTRFEVTGLMEDTQYQFRVYAVNKIGYS DPSDVPDKHYPKDILIPPEGELDADLRKTLILRAGVTMRLYVPVKGRPPP KITWSKPNVNLRDRIGLDIKSTDFDTFLRCENVNKYDAGKYILTLENSCG KKEYTIVVKVLDTPGPPVNVTVKEISKDSAYVTWEPPIIDGGSPIINYVV QKRDAERKSWSTVTTECSKTSFRVANLEEGKSYFFRVFAENEYGIGDPGE TRDAVKASQTPGPVVDLKVRSVSKSSCSIGWKKPHSDGGSRIIGYVVDFL TEENKWQRVMKSLSLQYSAKDLTEGKEYTFRVSAENENGEGTPSEITVVA RDDVVAPDLDLKGLPDLCYLAKENSNFRLKIPIKGKPAPSVSWKKGEDPL ATDTRVSVESSAVNTTLIVYDCQKSDAGKYTITLKNVAGTKEGTISIKVV GKPGIPTGPIKFDEVTAEAMTLKWAPPKDDGGSEITNYILEKRDSVNNKW VTCASAVQKTTFRVTRLHEGMEYTFRVSAENKYGVGEGLKSEPIVARHPF DVPDAPPPPNIVDVRHDSVSLTWTDPKKTGGSPITGYHLEFKERNSLLWK RANKTPIRMRDFKVTGLTEGLEYEFRVMAINLAGVGKPSLPSEPVVALDP IDPPGKPEVINITRNSVTLIWTEPKYDGGHKLTGYIVEKRDLPSKSWMKA NHVNVPECAFTVTDLVEGGKYEFRIRAKNTAGAISAPSESTETIICKDEY EAPTIVLDPTIKDGLTIKAGDTIVLNAISILGKPLPKSSWSKAGKDIRPS DITQITSTPTSSMLTIKYATRKDAGEYTITATNPFGTKVEHVKVTVLDVP GPPGPVEISNVSAEKATLTWTPPLEDGGSPIKSYILEKRETSRLLWTVVS EDIQSCRHVATKLIQGNEYIFRVSAVNHYGKGEPVQSEPVKMVDRFGPPG PPEKPEVSNVTKNTATVSWKRPVDDGGSEITGYHVERREKKSLRWVRAIK TPVSDLRCKVTGLQEGSTYEFRVSAENRAGIGPPSEASDSVLMKDAAYPP GPPSNPHVTDTTKKSASLAWGKPHYDGGLEITGYVVEHQKVGDEAWIKDT TGTALRITQFVVPDLQTKEKYNFRISAINDAGVGEPAVIPDVEIVEREMA PDFELDAELRRTLVVRAGLSIRIFVPIKGRPAPEVTWTKDNINLKNRANI ENTESFTLLIIPECNRYDTGKFVMTIENPAGKKSGFVNVRVLDTPGPVLN LRPTDITKDSVTLHWDLPLIDGGSRITNYIVEKREATRKSYSTATTKCHK CTYKVTGLSEGCEYFFRVMAENEYGIGEPTETTEPVKASEAPSPPDSLNI MDITKSTVSLAWPKPKHDGGSKITGYVIEAQRKGSDQWTHITTVKGLECV VRNLTEGEEYTFQVMAVNSAGRSAPRESRPVIVKEQTMLPELDLRGIYQK LVIAKAGDNIKVEIPVLGRPKPTVTWKKGDQILKQTQRVNFETTATSTIL NINECVRSDSGPYPLTARNIVGEVGDVITIQVHDIPGPPTGPIKFDEVSS DFVTFSWDPPENDGGVPISNYVVEMRQTDSTTWVELATTVIRTTYKATRL TTGLEYQFRVKAQNRYGVGPGITSACIVANYPFKVPGPPGTPQVTAVTKD SMTISWHEPLSDGGSPILGYHVERKERNGILWQTVSKALVPGNIFKSSGL TDGIAYEFRVIAENMAGKSKPSKPSEPMLALDPIDPPGKPVPLNITRHTV TLKWAKPEYTGGFKITSYIVEKRDLPNGRWLKANFSNILENEFTVSGLTE DAAYEFRVIAKNAAGAISPPSEPSDAITCRDDVEAPKIKVDVKFKDTVIL KAGEAFRLEADVSGRPPPTMEWSKDGKELEGTAKLEIKIADFSTNLVNKD STRRDSGAYTLTATNPGGFAKHIFNVKVLDRPGPPEGPLAVTEVTSEKCV LSWFPPLDDGGAKIDHYIVQKRETSRLAWTNVASEVQVTKLKVTKLLKGN EYIFRVMAVNKYGVGEPLESEPVLAVNPYGPPDPPKNPEVTTITKDSMVV CWGHPDSDGGSEIINYIVERRDKAGQRWIKCNKKTLTDLRYKVSGLTEGH EYEFRIMAENAAGISAPSPTSPFYKACDTVFKPGPPGNPRVLDTSRSSIS IAWNKPIYDGGSEITGYMVEIALPEEDEWQIVTPPAGLKATSYTITGLTE NQEYKIRIYAMNSEGLGEPALVPGTPKAEDRMLPPEIELDADLRKVVTIR ACCTLRLFVPIKGRPAPEVKWARDHGESLDKASIESTSSYTLLIVGNVNR FDSGKYILTVENSSGSKSAFVNVRVLDTPGPPQDLKVKEVTKTSVTLTWD PPLLDGGSKIKNYIVEKRESTRKAYSTVATNCHKTSWKVDQLQEGCSYYF RVLAENEYGIGLPAETAESVKASERPLPPGKITLMDVTRNSVSLSWEKPE HDGGSRILGYIVEMQTKGSDKWATCATVKVTEATITGLIQGEEYSFRVSA QNEKGISDPRQLSVPVIAKDLVIPPAFKLLFNTFTVLAGEDLKVDVPFIG RPTPAVTWHKDNVPLKQTTRVNAESTENNSLLTIKDACREDVGHYVVKLT NSAGEAIETLNVIVLDKPGPPTGPVKMDEVTADSITLSWGPPKYDGGSSI NNYIVEKRDTSTTTWQIVSATVARTTIKACRLKTGCEYQFRIAAENRYGK STYLNSEPTVAQYPFKVPGPPGTPVVTLSSRDSMEVQWNEPISDGGSRVI GYHLERKERNSILWVKLNKTPIPQTKFKTTGLEEGVEYEFRVSAENIVGI GKPSKVSECYVARDPCDPPGRPEAIIVTRNSVTLQWKKPTYDGGSKITGY IVEKKELPEGRWMKASFTNIIDTHFEVTGLVEDHRYEFRVIARNAAGVFS EPSESTGAITARDEVDPPRISMDPKYKDTIVVHAGESFKVDADIYGKPIP TIQWIKGDQELSNTARLEIKSTDFATSLSVKDAVRVDSGNYILKAKNVAG ERSVTVNVKVLDRPGPPEGPVVISGVTAEKCTLAWKPPLQDGGSDIINYI VERRETSRLVWTVVDANVQTLSCKVTKLLEGNEYTFRIMAVNKYGVGEPL ESEPVVAKNPFVVPDAPKAPEVTTVTKDSMIVVWERPASDGGSEILGYVL EKRDKEGIRWTRCHKRLIGELRLRVTGLIENHDYEFRVSAENAAGLSEPS PPSAYQKACDPIYKPGPPNNPKVIDITRSSVFLSWSKPIYDGGCEIQGYI VEKCDVSVGEWTMCTPPTGINKTNIEVEKLLEKHEYNFRICAINKAGVGE HADVPGPIIVEEKLEAPDIDLDLELRKIINIRAGGSLRLFVPIKGRPTPE VKWGKVDGEIRDAAIIDVTSSFTSLVLDNVNRYDSGKYTLTLENSSGTKS AFVTVRVLDTPSPPVNLKVTEITKDSVSITWEPPLLDGGSKIKNYIVEKR EATRKSYAAVVTNCHKNSWKIDQLQEGCSYYFRVTAENEYGIGLPAQTAD PIKVAEVPQPPGKITVDDVTRNSVSLSWTKPEHDGGSKIIQYIVEMQAKH SEKWSECARVKSLQAVITNLTQGEEYLFRVVAVNEKGRSDPRSLAVPIVA KDLVIEPDVKPAFSSYSVQVGQDLKIEVPISGRPKPTITWTKDGLPLKQT TRINVTDSLDLTTLSIKETHKDDGGQYGITVANVVGQKTASIEIVTLDKP DPPKGPVKFDDVSAESITLSWNPPLYTGGCQITNYIVQKRDTTTTVWDVV SATVARTTLKVTKLKTGTEYQFRIFAENRYGQSFALESDPIVAQYPYKEP GPPGTPFATAISKDSMVIQWHEPVNNGGSPVIGYHLERKERNSILWTKVN KTIIHDTQFKAQNLEEGIEYEFRVYAENIVGVGKASKNSECYVARDPCDP PGTPEPIMVKRNEITLQWTKPVYDGGSMITGYIVEKRDLPDGRWMKASFT NVIETQFTVSGLTEDQRYEFRVIAKNAAGAISKPSDSTGPITAKDEVELP RISMDPKFRDTIVVNAGETFRLEADVHGKPLPTIEWLRGDKEIEESARCE IKNTDFKALLIVKDAIRIDGGQYILRASNVAGSKSFPVNVKVLDRPGPPE GPVQVTGVTSEKCSLTWSPPLQDGGSDISHYVVEKRETSRLAWTVVASEV VTNSLKVTKLLEGNEYVFRIMAVNKYGVGEPLESAPVLMKNPFVLPGPPK SLEVTNIAKDSMTVCWNRPDSDGGSEIIGYIVEKRDRSGIRWIKCNKRRI TDLRLRVTGLTEDHEYEFRVSAENAAGVGEPSPATVYYKACDPVFKPGPP TNAHIVDTTKNSITLAWGKPIYDGGSEILGYVVEICKADEEEWQIVTPQT GLRVTRFEISKLTEHQEYKIRVCALNKVGLGEATSVPGTVKPEDKLEAPE LDLDSELRKGIVVRAGGSARIHIPFKGRPTPEITWSREEGEFTDKVQIEK GVNYTQLSIDNCDRNDAGKYILKLENSSGSKSAFVTVKVLDTPGPPQNLA VKEVRKDSAFLVWEPPIIDGGAKVKNYVIDKRESTRKAYANVSSKCSKTS FKVENLTEGAIYYFRVMAENEFGVGVPVETVDAVKAAEPPSPPGKVTLTD VSQTSASLMWEKPEHDGGSRVLGYVVEMQPKGTEKWSIVAESKVCNAVVT GLSSGQEYQFRVKAYNEKGKSDPRVLGVPVIAKDLTIQPSLKLPFNTYSI QAGEDLKIEIPVIGRPRPNISWVKDGEPLKQTTRVNVEETATSTVLHIKE GNKDDFGKYTVTATNSAGTATENLSVIVLEKPGPPVGPVRFDEVSADFVV ISWEPPAYTGGCQISNYIVEKRDTTTTTWHMVSATVARTTIKITKLKTGT EYQFRIFAENRYGKSAPLDSKAVIVQYPFKEPGPPGTPFVTSISKDQMLV QWHEPVNDGGTKIIGYHLEQKEKNSILWVKLNKTPIQDTKFKTTGLDEGL EYEFKVSAENIVGIGKPSKVSECFVARDPCDPPGRPEAIVITRNNVTLKW KKPAYDGGSKITGYIVEKKDLPDGRWMKASFTNVLETEFTVSGLVEDQRY EFRVIARNAAGNFSEPSDSSGAITARDEIDAPNASLDPKYKDVIVVHAGE TFVLEADIRGKPIPDVVWSKDGKELEETAARMEIKSTIQKTTLVVKDCIR TDGGQYILKLSNVGGTKSIPITVKVLDRPGPPEGPLKVTGVTAEKCYLAW NPPLQDGGANISHYIIEKRETSRLSWTQVSTEVQALNYKVTKLLPGNEYI FRVMAVNKYGIGEPLESGPVTACNPYKPPGPPSTPEVSAITKDSMVVTWA RPVDDGGTEIEGYILEKRDKEGVRWTKCNKKTLTDLRLRVTGLTEGHSYE FRVAAENAAGVGEPSEPSVFYRACDALYPPGPPSNPKVTDTSRSSVSLAW SKPIYDGGAPVKGYVVEVKEAAADEWTTCTPPTGLQGKQFTVTKLKENTE YNFRICAINSEGVGEPATLPGSVVAQERIEPPEIELDADLRKVVVLRASA TLRLFVTIKGRPEPEVKWEKAEGILTDRAQIEVTSSFTMLVIDNVTRFDS GRYNLTLENNSGSKTAFVNVRVLDSPSAPVNLTIREVKKDSVTLSWEPPL IDGGAKITNYIVEKRETTRKAYATITNNCTKTTFRIENLQEGCSYYFRVL ASNEYGIGLPAETTEPVKVSEPPLPPGRVTLVDVTRNTATIKWEKPESDG GSKITGYVVEMQTKGSEKWSTCTQVKTLEATISGLTAGEEYVFRVAAVNE KGRSDPRQLGVPVIARDIEIKPSVELPFHTFNVKAREQLKIDVPFKGRPQ ATVNWRKDGQTLKETTRVNVSSSKTVTSLSIKEASKEDVGTYELCVSNSA GSITVPITIIVLDRPGPPGPIRIDEVSCDSITISWNPPEYDGGCQISNYI VEKKETTSTTWHIVSQAVARTSIKIVRLTTGSEYQFRVCAENRYGKSSYS ESSAVVAEYPFSPPGPPGTPKVVHATKSTMLVTWQVPVNDGGSRVIGYHL EYKERSSILWSKANKILIADTQMKVSGLDEGLMYEYRVYAENIAGIGKCS KSCEPVPARDPCDPPGQPEVTNITRKSVSLKWSKPHYDGGAKITGYIVER RELPDGRWLKCNYTNIQETYFEVTELTEDQRYEFRVFARNAADSVSEPSE STGPIIVKDDVEPPRVMMDVKFRDVIVVKAGEVLKINADIAGRPLPVISW AKDGIEIEERARTEIISTDNHTLLTVKDCIRRDTGQYVLTLKNVAGTRSV AVNCKVLDKPGPPAGPLEINGLTAEKCSLSWGRPQEDGGADIDYYIVEKR ETSHLAWTICEGELQMTSCKVTKLLKGNEYIFRVTGVNKYGVGEPLESVA IKALDPFTVPSPPTSLEITSVTKESMTLCWSRPESDGGSEISGYIIERRE KNSLRWVRVNKKPVYDLRVKSTGLREGCEYEYRVYAENAAGLSLPSETSP LIRAEDPVFLPSPPSKPKIVDSGKTTITIAWVKPLFDGGAPITGYTVEYK KSDDTDWKTSIQSLRGTEYTISGLTTGAEYVFRVKSVNKVGASDPSDSSD PQIAKEREEEPLFDIDSEMRKTLIVKAGASFTMTVPFRGRPVPNVLWSKP DTDLRTRAYVDTTDSRTSLTIENANRNDSGKYTLTIQNVLSAASLTLVVK VLDTPGPPTNITVQDVTKESAVLSWDVPENDGGAPVKNYHIEKREASKKA WVSVTNNCNRLSYKVTNLQEGAIYYFRVSGENEFGVGIPAETKEGVKITE KPSPPEKLGVTSISKDSVSLTWLKPEHDGGSRIVHYVVEALEKGQKNWVK CAVAKSTHHVVSGLRENSEYFFRVFAENQAGLSDPRELLLPVLIKEQLEP PEIDMKNFPSHTVYVRAGSNLKVDIPISGKPLPKVTLSRDGVPLKATMRF NTEITAENLTINLKESVTADAGRYEITAANSSGTTKAFINIVVLDRPGPP TGPVVISDITEESVTLKWEPPKYDGGSQVTNYILLKRETSTAVWTEVSAT VARTMMKVMKLTTGEEYQFRIKAENRFGISDHIDSACVTVKLPYTTPGPP STPWVTNVTRESITVGWHEPVSNGGSAVVGYHLEMKDRNSILWQKANKLV IRTTHFKVTTISAGLIYEFRVYAENAAGVGKPSHPSEPVLAIDACEPPRN VRITDISKNSVSLSWQQPAFDGGSKITGYIVERRDLPDGRWTKASFTNVT ETQFIISGLTQNSQYEFRVFARNAVGSISNPSEVVGPITCIDSYGGPVID LPLEYTEVVKYRAGTSVKLRAGISGKPAPTIEWYKDDKELQTNALVCVEN TTDLASILIKDADRLNSGCYELKLRNAMGSASATIRVQILDKPGPPGGPI EFKTVTAEKITLLWRPPADDGGAKITHYIVEKRETSRVVWSMVSEHLEEC IITTTKIIKGNEYIFRVRAVNKYGIGEPLESDSVVAKNAFVTPGPPGIPE VTKITKNSMTVVWSRPIADGGSDISGYFLEKRDKKSLGWFKVLKETIRDT RQKVTGLTENSDYQYRVCAVNAAGQGPFSEPSEFYKAADPIDPPGPPAKI RIADSTKSSITLGWSKPVYDGGSAVTGYVVEIRQGEEEEWTTVSTKGEVR TTEYVVSNLKPGVNYYFRVSAVNCAGQGEPIEMNEPVQAKDILEAPEIDL DVALRTSVIAKAGEDVQVLIPFKGRPPPTVTWRKDEKNLGSDARYSIENT DSSSLLTIPQVTRNDTGKYILTIENGVGEPKSSTVSVKVLDTPAACQKLQ VKHVSRGTVTLLWDPPLIDGGSPIINYVIEKRDATKRTWSVVSHKCSSTS FKLIDLSEKTPFFFRVLAENEIGIGEPCETTEPVKAAEVPAPIRDLSMKD STKTSVILSWTKPDFDGGSVITEYVVERKGKGEQTWSHAGISKTCEIEVS QLKEQSVLEFRVFAKNEKGLSDPVTIGPITVKELIITPEVDLSDIPGAQV TVRIGHNVHLELPYKGKPKPSISWLKDGLPLKESEFVRFSKTENKITLSI KNAKKEHGGKYTVILDNAVCRIAVPITVITLGPPSKPKGPIRFDEIKADS VILSWDVPEDNGGGEITCYSIEKRETSQTNWKMVCSSVARTTFKVPNLVK DAEYQFRVRAENRYGVSQPLVSSIIVAKHQFRIPGPPGKPVIYNVTSDGM SLTWDAPVYDGGSEVTGFHVEKKERNSILWQKVNTSPISGREYRATGLVE GLDYQFRVYAENSAGLSSPSDPSKFTLAVSPVDPPGTPDYIDVTRETITL KWNPPLRDGGSKIVGYSIEKRQGNERWVRCNFTDVSECQYTVTGLSPGDR YEFRIIARNAVGTISPPSQSSGIIMTRDENVPPIVEFGPEYFDGLIIKSG ESLRIKALVQGRPVPRVTWFKDGVEIEKRMNMEITDVLGSTSLFVRDATR DHRGVYTVEAKNASGSAKAEIKVKVQDTPGKVVGPIRFTNITGEKMTLWW DAPLNDGCAPITHYIIEKRETSRLAWALIEDKCEAQSYTAIKLINGNEYQ FRVSAVNKFGVGRPLDSDPVVAQIQYTVPDAPGIPEPSNITGNSITLTWA RPESDGGSEIQQYILERREKKSTRWVKVISKRPISETRFKVTGLTEGNEY EFHVMAENAAGVGPASGISRLIKCREPVNPPGPPTVVKVTDTSKTTVSLE WSKPVFDGGMEIIGYIIEMCKADLGDWHKVNAEACVKTRYTVTDLQAGEE YKFRVSAINGAGKGDSCEVTGTIKAVDRLTAPELDIDANFKQTHVVRAGA SIRLFIAYQGRPTPTAVWSKPDSNLSLRADIHTTDSFSTLTVENCNRNDA GKYTLTVENNSGSKSITFTVKVLDTPGPPGPITFKDVTRGSATLMWDAPL LDGGARIHHYVVEKREASRRSWQVISEKCTRQIFKVNDLAEGVPYYFRVS AVNEYGVGEPYEMPEPIVATEQPAPPRRLDVVDTSKSSAVLAWLKPDHDG GSRITGYLLEMRQKGSDFWVEAGHTKQLTFTVERLVEKTEYEFRVKAKND AGYSEPREAFSSVIIKEPQIEPTADLTGITNQLITCKAGSPFTIDVPISG RPAPKVTWKLEEMRLKETDRVSITTTKDRTTLTVKDSMRGDSGRYFLTLE NTAGVKTFSVTVVVIGRPGPVTGPIEVSSVSAESCVLSWGEPKDGGGTEI TNYIVEKRESGTTAWQLVNSSVKRTQIKVTHLTKYMEYSFRVSSENRFGV SKPLESAPIIAEHPFVPPSAPTRPEVYHVSANAMSIRWEEPYHDGGSKII GYWVEKKERNTILWVKENKVPCLECNYKVTGLVEGLEYQFRTYALNAAGV SKASEASRPIMAQNPVDAPGRPEVTDVTRSTVSLIWSAPAYDGGSKVVGY IIERKPVSEVGDGRWLKCNYTIVSDNFFTVTALSEGDTYEFRVLAKNAAG VISKGSESTGPVTCRDEYAPPKAELDARLHGDLVTIRAGSDLVLDAAVGG KPEPKIIWTKGDKELDLCEKVSLQYTGKRATAVIKFCDRSDSGKYTLTVK NASGTKAVSVMVKVLDSPGPCGKLTVSRVTQEKCTLAWSLPQEDGGAEIT HYIVERRETSRLNWVIVEGECPTLSYVVTRLIKNNEYIFRVRAVNKYGPG VPVESEPIVARNSFTIPSPPGIPEEVGTGKEHIIIQWTKPESDGGNEISN YLVDKREKKSLRWTRVNKDYVVYDTRLKVTSLMEGCDYQFRVTAVNAAGN SEPSEASNFISCREPSYTPGPPSAPRVVDTTKHSISLAWTKPMYDGGTDI VGYVLEMQEKDTDQWYRVHTNATIRNTEFTVPDLKMGQKYSFRVAAVNVK GMSEYSESIAEIEPVERIEIPDLELADDLKKTVTIRAGASLRLMVSVSGR PPPVITWSKQGIDLASRAIIDTTESYSLLIVDKVNRYDAGKYTIEAENQS GKKSATVLVKVYDTPGPCPSVKVKEVSRDSVTITWEIPTIDGGAPVNNYI VEKREAAMRAFKTVTTKCSKTLYRISGLVEGTMYYFRVLPENIYGIGEPC ETSDAVLVSEVPLVPAKLEVVDVTKSTVTLAWEKPLYDGGSRLTGYVLEA CKAGTERWMKVVTLKPTVLEHTVTSLNEGEQYLFRIRAQNEKGVSEPRET VTAVTVQDLRVLPTIDLSTMPQKTIHVPAGRPVELVIPIAGRPPPAASWF FAGSKLRESERVTVETHTKVAKLTIRETTIRDTGEYTLELKNVTGTTSET IKVIILDKPGPPTGPIKIDEIDATSITISWEPPELDGGAPLSGYVVEQRD AHRPGWLPVSESVTRSTFKFTRLTEGNEYVFRVAATNRFGIGSYLQSEVI ECRSSIRIPGPPETLQIFDVSRDGMTLTWYPPEDDGGSQVTGYIVERKEV RADRWVRVNKVPVTMTRYRSTGLTEGLEYEHRVTAINARGSGKPSRPSKP IVAMDPIAPPGKPQNPRVTDTTRTSVSLAWSVPEDEGGSKVTGYLIEMQK VDQHEWTKCNTTPTKIREYTLTHLPQGAEYRFRVLACNAGGPGEPAEVPG TVKVTEMLEYPDYELDERYQEGIFVRQGGVIRLTIPIKGKPFPICKWTKE GQDISKRAMIATSETHTELVIKEADRGDSGTYDLVLENKCGKKAVYIKVR VIGSPNSPEGPLEYDDIQVRSVRVSWRPPADDGGADILGYILERREVPKA AWYTIDSRVRGTSLVVKGLKENVEYHFRVSAENQFGISKPLKSEEPVTPK TPLNPPEPPSNPPEVLDVTKSSVSLSWSRPKDDGGSRVTGYYIERKETST DKWVRHNKTQITTTMYTVTGLVPDAEYQFRIIAQNDVGLSETSPASEPVV CKDPFDKPSQPGELEILSISKDSVTLQWEKPECDGGKEILGYWVEYRQSG DSAWKKSNKERIKDKQFTIGGLLEATEYEFRVFAENETGLSRPRRTAMSI KTKLTSGEAPGIRKEMKDVTTKLGEAAQLSCQIVGRPLPDIKWYRFGKEL IQSRKYKMSSDGRTHTLTVMTEEQEDEGVYTCIATNEVGEVETSSKLLLQ ATPQFHPGYPLKEKYYGAVGSTLRLHVMYIGRPVPAMTWFHGQKLLQNSE NITIENTEHYTHLVMKNVQRKTHAGKYKVQLSNVFGTVDAILDVEIQDKP DKPTGPIVIEALLKNSAVISWKPPADDGGSWITNYVVEKCEAKEGAEWQL VSSAISVTTCRIVNLTENAGYYFRVSAQNTFGISDPLEVSSVVIIKSPFE KPGAPGKPTITAVTKDSCVVAWKPPASDGGAKIRNYYLEKREKKQNKWIS VTTEEIRETVFSVKNLIEGLEYEFRVKCENLGGESEWSEISEPITPKSDV PIQAPHFKEELRNLNVRYQSNATLVCKVTGHPKPIVKWYRQGKEIIADGL KYRIQEFKGGYHQLIIASVTDDDATVYQVRATNQGGSVSGTASLEVEVPA KIHLPKTLEGMGAVHALRGEVVSIKIPFSGKPDPVITWQKGQDLIDNNGH YQVIVTRSFTSLVFPNGVERKDAGFYVVCAKNRFGIDQKTVELDVADVPD PPRGVKVSDVSRDSVNLTWTEPASDGGSKITNYIVEKCATTAERWLRVGQ ARETRYTVINLFGKTSYQFRVIAENKFGLSKPSEPSEPTITKEDKTRAMN YDEEVDETREVSMTKASHSSTKELYEKYMIAEDLGRGEFGIVHRCVETSS KKTYMAKFVKVKGTDQVLVKKEISILNIARHRNILHLHESFESMEELVMI FEFISGLDIFERINTSAFELNEREIVSYVHQVCEALQFLHSHNIGHFDIR PENIIYQTRRSSTIKIIEFGQARQLKPGDNFRLLFTAPEYYAPEVHQHDV VSTATDMWSLGTLVYVLLSGINPFLAETNQQIIENIMNAEYTFDEEAFKE ISIEAMDFVDRLLVKERKSRMTASEALQHPWLKQKIERVSTKVIRTLKHR RYYHTLIKKDLNMVVSAARISCGGAIRSQKGVSVAKVKVASIEIGPVSGQ IMHAVGEEGGHVKYVCKIENYDQSTQVTWYFGVRQLENSEKYEITYEDGV AILYVKDITKLDDGTYRCKVVNDYGEDSSYAELFVKGVREVYDYYCRRTM KKIKRRTDTMRLLERPPEFTLPLYNKTAYVGENVRFGVTITVHPEPHVTW YKSGQKIKPGDNDKKYTFESDKGLYQLTINSVTTDDDAEYTVVARNKYGE DSCKAKLTVTLHPPPTDSTLRPMFKRLLANAECQEGQSVCFEIRVSGIPP PTLKWEKDGQPLSLGPNIEIIHEGLDYYALHIRDTLPEDTGYYRVTATNT AGSTSCQAHLQVERLRYKKQEFKSKEEHERHVQKQIDKTLRMAEILSGTE SVPLTQVAKEALREAAVLYKPAVSTKTVKGEFRLEIEEKKEERKLRMPYD VPEPRKYKQTTIEEDQRIKQFVPMSDMKWYKKIRDQYEMPGKLDRVVQKR PKRIRLSRWEQFYVMPLPRITDQYRPKWRIPKLSQDDLEIVRPARRRTPS PDYDFYYRPRRRSLGDISDEELLLPIDDYLAMKRTEEERLRLEEELELGF SASPPSRSPPHFELSSLRYSSPQAHVKVEETRKDFRYSTYHIPTKAEAST SYAELRERHAQAAYRQPKQRQRIMAEREDEELLRPVTTTQHLSEYKSELD FMSKEEKSRKKSRRQREVTEITEIEEEYEISKHAQRESSSSASRLLRRRR SLSPTYIELMRPVSELIRSRPQPAEEYEDDTERRSPTPERTRPRSPSPVS SERSLSRFERSARFDIFSRYESMKAALKTQKTSERKYEVLSQQPFTLDHA PRITLRMRSHRVPCGQNTRFILNVQSKPTAEVKWYHNGVELQESSKIHYT NTSGVLTLEILDCHTDDSGTYRAVCTNYKGEASDYATLDVTGGDYTTYAS QRRDEEVPRSVFPELTRTEAYAVSSFKKTSEMEASSSVREVKSQMTETRE SLSSYEHSASAEMKSAALEEKSLEEKSTTRKIKTTLAARILTKPRSMTVY EGESARFSCDTDGEPVPTVTWLRKGQVLSTSARHQVTTTKYKSTFEISSV QASDEGNYSVVVENSEGKQEAEFTLTIQKARVTEKAVTSPPRVKSPEPRV KSPEAVKSPKRVKSPEPSHPKAVSPTETKPTPTEKVQHLPVSAPPKITQF LKAEASKEIAKLTCVVESSVLRAKEVTWYKDGKKLKENGHFQFHYSADGT YELKINNLTESDQGEYVCEISGEGGTSKTNLQFMGQAFKSIHEKVSKISE TKKSDQKTTESTVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAV KATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYT CTVKNSAGSVSSSCKLTIKAIKDTEAQKVSTQKTSEITPQKKAVVQEEIS QKALRSEEIKMSEAKSQEKLALKEEASKVLISEEVKKSAATSLEKSIVHE EITKTSQASEEVRTHAEIKAFSTQMSINEGQRLVLKANIAGATDVKWVLN GVELTNSEEYRYGVSGSDQTLTIKQASHRDEGILTCISKTKEGIVKCQYD LTLSKELSDAPAFISQPRSQNINEGQNVLFTCEISGEPSPEIEWFKNNLP ISISSNVSISRSRNVYSLEIRNASVSDSGKYTIKAKNFRGQCSATASLMV LPLVEEPSREVVLRTSGDTSLQGSFSSQSVQMSASKQEASFSSFSSSSAS SMTEMKFASMSAQSMSSMQESFVEMSSSSFMGISNMTQLESSTSKMLKAG IRGIPPKIEALPSDISIDEGKVLTVACAFTGEPTPEVTWSCGGRKIHSQE QGRFHIENTDDLTTLIIMDVQKQDGGLYTLSLGNEFGSDSATVNIHIRSI tantan-22/test/panda.fastq0000644000000000000000000005003213406427735014377 0ustar rootroot@SRR019778.1 I331_2_FC3076HAAXX:1:1:1416:1963 length=45 GTTCAATGGGGGAAAGGAGAGTCCTTCAACAAATGGTGCGGAGAA +SRR019778.1 I331_2_FC3076HAAXX:1:1:1416:1963 length=45 IIIIIIIIIIIIIIIIIIIIIIIIIIIB2IHH(-0+ @SRR019778.3 I331_2_FC3076HAAXX:1:1:1611:1333 length=45 GTTTTCCCATTTGTCCTTAATCCACCTAAAAAAGATTTGCATGGG +SRR019778.3 I331_2_FC3076HAAXX:1:1:1611:1333 length=45 IIIIIIIIIIIIIEIIIIG:IDIGECI454,10+0A?8/4+40%( @SRR019778.4 I331_2_FC3076HAAXX:1:1:1277:1927 length=45 GTTGTGTGTGTATTGTGTGTTGTGTGTGGTGTGTGTGTTTTTTTT +SRR019778.4 I331_2_FC3076HAAXX:1:1:1277:1927 length=45 IIIIIIIIIIIIII6IIIIIIDIII4I%4I*I0IAI+II8/3I+I @SRR019778.5 I331_2_FC3076HAAXX:1:1:1494:1984 length=45 GGATGTTTATTTTCCTGAACCAGGGTTTGGCATTGAAGAGACAGA +SRR019778.5 I331_2_FC3076HAAXX:1:1:1494:1984 length=45 IIIIIIIIIIIIIIIIIIIII/III@III@<-I6I'3I67-++>) @SRR019778.6 I331_2_FC3076HAAXX:1:1:682:1158 length=45 GTTTGTTTCCTAGAGTTAAGAGTCTCTCATGGTTTGTCTCCCTCT +SRR019778.6 I331_2_FC3076HAAXX:1:1:682:1158 length=45 IIIIIIIIIIIII:IIII=IEIIHI@IC2I9;I@@//?4DA5080,&, @SRR019778.13 I331_2_FC3076HAAXX:1:1:1245:2003 length=45 GTTTCCATAACCAGAAATATACAGCGGATTAGAGAAGTGATTTTT +SRR019778.13 I331_2_FC3076HAAXX:1:1:1245:2003 length=45 IIIIIIIIIIIIIIG>1I4I5I9IIII*II.I+G)-B7G&:B@C@ @SRR019778.14 I331_2_FC3076HAAXX:1:1:1226:1990 length=45 GGGAGGATCTAGCCTTAGAACCAAGAAACAAAATGTGAGGGGAAA +SRR019778.14 I331_2_FC3076HAAXX:1:1:1226:1990 length=45 IIIIIIIIIIIIIIII6IIIIIAII>.III-3/II3I1IIII.-- @SRR019778.15 I331_2_FC3076HAAXX:1:1:1879:1017 length=45 GTGATGTGCCATTGACCTATTTGTTTATCTTTATACCAATACTTC +SRR019778.15 I331_2_FC3076HAAXX:1:1:1879:1017 length=45 IIIIIIIIIIIIII2III/IIDGFII/I7IG:5CC;/0&=)+;4+ @SRR019778.16 I331_2_FC3076HAAXX:1:1:1478:1340 length=45 GTATTTTACAGAAGTTTCTCTGGGTCTCAGATTCCTACCTGACCC +SRR019778.16 I331_2_FC3076HAAXX:1:1:1478:1340 length=45 IIIIIIIIIIIIIIDIIIIII>;C;DI@D*3IE<2B/20;&'/-/ @SRR019778.17 I331_2_FC3076HAAXX:1:1:1489:1981 length=45 GGGAAAGATAGAGAGCCTTGTCTTCAAAATGGCTTATCCAGTAAG +SRR019778.17 I331_2_FC3076HAAXX:1:1:1489:1981 length=45 IIIIIIIIIIIIIIIIIIIIIIIII?+<5IIIIIB*66<%=3'(5 @SRR019778.18 I331_2_FC3076HAAXX:1:1:1710:940 length=45 GAGAATCCACAAGAAGTTGCTGTTAGGGATTTGTTCTTATTTTAA +SRR019778.18 I331_2_FC3076HAAXX:1:1:1710:940 length=45 IIIIIIIIIIIII0CIIIIIII@I(-?6-@?C.37,A:%D;:&** @SRR019778.19 I331_2_FC3076HAAXX:1:1:1339:973 length=45 GAAAAGGCATAAATATTTCATTCAATTGCAAGCTTAATATAAGTC +SRR019778.19 I331_2_FC3076HAAXX:1:1:1339:973 length=45 IIIIIIIIIIIIIIFIIIIHIIII:II;II63=IIC.GFI76(.. @SRR019778.20 I331_2_FC3076HAAXX:1:1:1408:1952 length=45 GAGTTTTCCATTTAAGAACTTGAGTGTCATCGCCTTAGGAAAGAC +SRR019778.20 I331_2_FC3076HAAXX:1:1:1408:1952 length=45 IIIIIIIIIIIII@FIFGIIII8I?+HIIDI=E>I>8?1)1&*// @SRR019778.21 I331_2_FC3076HAAXX:1:1:1468:1371 length=45 GAAGAGAAAGGAGATACCATCATTTGTCATTAACAGGATTACCTT +SRR019778.21 I331_2_FC3076HAAXX:1:1:1468:1371 length=45 IIIIIIIIIIIIIIIIIIIIIFIIII=I3I:13=A<@,/03I2<+:- @SRR019778.26 I331_2_FC3076HAAXX:1:1:1225:1945 length=45 GTAATGATTGAATAAATCTTATTAACTTCTTAATCTTTTAATTTT +SRR019778.26 I331_2_FC3076HAAXX:1:1:1225:1945 length=45 IIIIIIIIIIIIIIIIIIIIIIIB4HIIIII:3IIIIII5)IIGI @SRR019778.27 I331_2_FC3076HAAXX:1:1:1506:1294 length=45 GCTTCAAGAAGAAGGGCATGACTCACCCCCCAGGAACAGAAGCTG +SRR019778.27 I331_2_FC3076HAAXX:1:1:1506:1294 length=45 IIIIIIIIIIIIIIIIIIIIIIII@IIICDB15B+39.9*.B4:5 @SRR019778.28 I331_2_FC3076HAAXX:1:1:1318:1418 length=45 GATTTTATTCTTATTGTTTTGATGGTGACCTTTAATATTTTCTTA +SRR019778.28 I331_2_FC3076HAAXX:1:1:1318:1418 length=45 IIIIIIIIIIIIIIIIIIIIADI,BFIA..IIIC2$7IFEC1&;) @SRR019778.29 I331_2_FC3076HAAXX:1:1:1332:1930 length=45 GAGAATAAGTCAATACCAAGGTGAATTGAATAATGGCCAAGAATT +SRR019778.29 I331_2_FC3076HAAXX:1:1:1332:1930 length=45 IIIIIIIIIIIIIIIIGIHIIII58IIII5I@9AI8I0=,B,-3> @SRR019778.30 I331_2_FC3076HAAXX:1:1:1099:1168 length=45 GTTTATTCTCTATCATTTAGAGTCTCAGCTGTTTGGGTTTCTAGA +SRR019778.30 I331_2_FC3076HAAXX:1:1:1099:1168 length=45 IIIIIIIIIIIIII;IIII8I+BFII+C.2+8I.,&%3$$3/$)$ @SRR019778.31 I331_2_FC3076HAAXX:1:1:1529:1292 length=45 GGATAGCCCCAAGTGTTACAAATTGTAAGTAAATATTTGTAGGGA +SRR019778.31 I331_2_FC3076HAAXX:1:1:1529:1292 length=45 IIIIIIIIIIIIIIIII>I<<8IIIID2I=/7.;2EH@22/6+&* @SRR019778.32 I331_2_FC3076HAAXX:1:1:1256:1996 length=45 GTTGGGGATTAGAAAGTAGATGTAAGGAGTAGTAAAGGGAATACA +SRR019778.32 I331_2_FC3076HAAXX:1:1:1256:1996 length=45 IIIIIIIIIIIIIHIIIIIIIIHI6II5I;I3 @SRR019778.39 I331_2_FC3076HAAXX:1:1:1136:1244 length=45 GTTATATGTGAGTAATGAACCATTGAACACTACACCAAAAATTAA +SRR019778.39 I331_2_FC3076HAAXX:1:1:1136:1244 length=45 IIIIIIIIIIIIIIIIIIIIDIIIII:8B8IH3;72<8031D92- @SRR019778.40 I331_2_FC3076HAAXX:1:1:1819:927 length=45 GGGTTTGGAGTTTAAAGAGAGAATAAGTCATGTGCCAATATATGA +SRR019778.40 I331_2_FC3076HAAXX:1:1:1819:927 length=45 IIIIIIIIIIIIIIG;IFIIII6II?IEBE?8@A<79,D5<4F1) @SRR019778.41 I331_2_FC3076HAAXX:1:1:1250:1938 length=45 GTTTGTGTCCCTCTAAAGTTCATATACTGAAGCCCTAACCCCCAG +SRR019778.41 I331_2_FC3076HAAXX:1:1:1250:1938 length=45 IIIIIIIIIIIIIIII2IIIIIIIIIIII=:=IIEI3/I7I7G+0 @SRR019778.42 I331_2_FC3076HAAXX:1:1:1648:1646 length=45 GGTTGTGTGTGTGTGTGTGTGTGTGTGTGTTTGTTTTTTTTTTTA +SRR019778.42 I331_2_FC3076HAAXX:1:1:1648:1646 length=45 IIIIIIIIIIIIIIIIIIII7I@I.IDIB,.@%I0*-E(G%(/.% @SRR019778.43 I331_2_FC3076HAAXX:1:1:690:962 length=45 GTGAGATGATGTTCGTTGATATTTCCCTGTATTTTTTGTTTTTTC +SRR019778.43 I331_2_FC3076HAAXX:1:1:690:962 length=45 IIIIIIIIIIIIIIIIII>I?III??,I'$)II%:&B&'8(9$=$ @SRR019778.44 I331_2_FC3076HAAXX:1:1:1399:1957 length=45 GTTGATATTTCCCTGGACTGTGTGGGGTCTCAGAGACCACTATGG +SRR019778.44 I331_2_FC3076HAAXX:1:1:1399:1957 length=45 IIIIIIIIIIIIIIII=IIIIIIIIIIIIIG4>,I/9;*3D*:<+ @SRR019778.45 I331_2_FC3076HAAXX:1:1:1797:1797 length=45 GTGTGTATTGTGTGTGGGTGTGTGTGTTGTGTGTGTTTTGGGTGG +SRR019778.45 I331_2_FC3076HAAXX:1:1:1797:1797 length=45 IIIIIIIIIIIIII@IIIIIIIAII?II?I0(+D)II$:&5%'&% @SRR019778.46 I331_2_FC3076HAAXX:1:1:721:921 length=45 GATGTCATAGAAGTTGTTGCCTATGTTCTCCTCTAGAATTTTGAT +SRR019778.46 I331_2_FC3076HAAXX:1:1:721:921 length=45 IIIIIIIIIIIIIIIIIIH?CI6I7II0I11F5I+&+(ICIH&&= @SRR019778.47 I331_2_FC3076HAAXX:1:1:1584:1300 length=45 GGTAGTGAGTATCAGCCCTGTGCAGGTTTCTGCAGATGGCCATGT +SRR019778.47 I331_2_FC3076HAAXX:1:1:1584:1300 length=45 IIIII-IIIIIIIHIIIII4;GCIEI-II320-&-#)%,.,$-%$ @SRR019778.48 I331_2_FC3076HAAXX:1:1:1397:1963 length=45 GAAAACTTACTTACAAATTCATTCTATGAGGCCAGCATTACCCTG +SRR019778.48 I331_2_FC3076HAAXX:1:1:1397:1963 length=45 IIIIIIIIIIIIIIII:IIIIIIII4II4FIIH0F@*CI47><:1 @SRR019778.49 I331_2_FC3076HAAXX:1:1:1550:1296 length=45 GGTAGATGTGACTCTTGATCTCAGGGTCATGAGTTCAAGCCCCAT +SRR019778.49 I331_2_FC3076HAAXX:1:1:1550:1296 length=45 IIIIIIIIIIIIIIIIICIIBI6D%I9B5C;0I+;37-4)0**)3 @SRR019778.50 I331_2_FC3076HAAXX:1:1:913:1939 length=45 GTGTGTTGTGTGTTGAGTGTTTTGTGTGTGTGTTGTGTTTTTTGT +SRR019778.50 I331_2_FC3076HAAXX:1:1:913:1939 length=45 IIIIIIIIIIIIIII2IIIIIII@IIG2I.I,II&9&4%II,:%, @SRR019778.51 I331_2_FC3076HAAXX:1:1:1734:1009 length=45 GAAACAATGGTTTTCAAGACACTGGACATCAGCCTACAATGGACA +SRR019778.51 I331_2_FC3076HAAXX:1:1:1734:1009 length=45 IIIIIIIIIIIIIIIIIIEIBIIID.>2I?186C?)/&-8,1)1+ @SRR019778.52 I331_2_FC3076HAAXX:1:1:1355:100 length=45 GTACACAAATCTACGTGATATTTCGAAAGGTAAACAGATAATGCT +SRR019778.52 I331_2_FC3076HAAXX:1:1:1355:100 length=45 IIIIIIIIIIIII7I7,IIC&FD>1->9'+00 @SRR019778.56 I331_2_FC3076HAAXX:1:1:1759:1586 length=45 GGTACTCTAATAGCGTTTTATGGTAAGAGATGGTAGCTGTACTTG +SRR019778.56 I331_2_FC3076HAAXX:1:1:1759:1586 length=45 IIIIIIIIIF0I1III++;6.':594*7)4* @SRR019778.58 I331_2_FC3076HAAXX:1:1:1371:1534 length=45 GTGATGGGAAAGGAAAGAGCCAATCCTGTAGGGCCTGGTAGATCA +SRR019778.58 I331_2_FC3076HAAXX:1:1:1371:1534 length=45 I7IIIIIIIIIIIIIIIIIIII>III@*86IIIF??I7+)6-9+0 @SRR019778.59 I331_2_FC3076HAAXX:1:1:1780:1771 length=45 GTAAAATGGAGTAAATAGAGTAGAATAACATAAAAATTACAATGT +SRR019778.59 I331_2_FC3076HAAXX:1:1:1780:1771 length=45 IIIIIIIIIIIII;IIIE.-/0827:4./3- @SRR019778.60 I331_2_FC3076HAAXX:1:1:1539:1636 length=45 GTGTTGCTCTTTCTGAGGTGGTAATTGTGGTTTCCTTGGGCCTTT +SRR019778.60 I331_2_FC3076HAAXX:1:1:1539:1636 length=45 IIIIIIIIIIIIIIIIIICII:7III9I9IFII1(4I%%+&*1&. @SRR019778.61 I331_2_FC3076HAAXX:1:1:1360:1444 length=45 GTTGGGTTGATTTTCATTGGTGCTAATGTAATGGGGTATTGATCC +SRR019778.61 I331_2_FC3076HAAXX:1:1:1360:1444 length=45 IIIIIIIIIIIIIIIIIIIBI/GI>1II%).I),,+9'IB)+B+) @SRR019778.62 I331_2_FC3076HAAXX:1:1:1763:997 length=45 GAGATGAGTCAGCAGAGGTAAAAGCTAGAAGGATGTTAGGATGAT +SRR019778.62 I331_2_FC3076HAAXX:1:1:1763:997 length=45 IIIIIIIIIIIII?I@IID=II@A?E?I1;D=:C@6>-<0+A2.= @SRR019778.63 I331_2_FC3076HAAXX:1:1:996:1270 length=45 GTCAGGGACTTAATATCTTTCCCTTAAAATATGTGTGTTATTTTC +SRR019778.63 I331_2_FC3076HAAXX:1:1:996:1270 length=45 IIIIIIIIIIIIIIIIIIIIIEIII<:9)<(:I%: @SRR019778.66 I331_2_FC3076HAAXX:1:1:1314:76 length=45 GTGCTTAGCAGATAGGAGGGGCTCATTAACATGTTGAACAAATGT +SRR019778.66 I331_2_FC3076HAAXX:1:1:1314:76 length=45 IIIIIIIIIIIII7II7IIII?II4II/14+I*I@/.:/.6*F>>+ @SRR019778.68 I331_2_FC3076HAAXX:1:1:1459:1955 length=45 GTGTTCCCATACTTTAACTTGTGTGTGTTGTGGGGGGAAGCATAT +SRR019778.68 I331_2_FC3076HAAXX:1:1:1459:1955 length=45 IIIIIIIIDIIIIII>FIIIIIIIIIIII6I>47>I+*(/#$I$@ @SRR019778.69 I331_2_FC3076HAAXX:1:1:1381:1967 length=45 GCCCTAAGAACCAAGAAACAAAGTGTGAGGGGAAAGCCCTTGAAT +SRR019778.69 I331_2_FC3076HAAXX:1:1:1381:1967 length=45 IIIIIIIIIIIII:IE)CIC?1F4I+I8IIII.,/I959-:B&'9 @SRR019778.70 I331_2_FC3076HAAXX:1:1:1224:2001 length=45 GATACGTCAAATTTATGGAAGTGTTTTATAAGTGCATTTAAGTTT +SRR019778.70 I331_2_FC3076HAAXX:1:1:1224:2001 length=45 IIIIIIIIIIIIIIIIII8EI=IIIII8I4.9E9+$II&%+(:?& @SRR019778.71 I331_2_FC3076HAAXX:1:1:1771:1569 length=45 GTTTTTATAATGACTTCCTTAGGCTGTACCCCCAATTTACTATAT +SRR019778.71 I331_2_FC3076HAAXX:1:1:1771:1569 length=45 IIIIIIIIIIIIIIIIIIII*(IAB(4?'D'F @SRR019778.72 I331_2_FC3076HAAXX:1:1:1745:1606 length=45 GATTTGCAGTCAAGGACAGGTCCACACAGTCAGGACCACAGTGTT +SRR019778.72 I331_2_FC3076HAAXX:1:1:1745:1606 length=45 IIIIIIIIIIIIEIIII-I2; @SRR019778.73 I331_2_FC3076HAAXX:1:1:1279:1959 length=45 GGAGGAAGACCACAGAGCTGAAGCACCGTCCTCATCACATCAGGT +SRR019778.73 I331_2_FC3076HAAXX:1:1:1279:1959 length=45 IIIIIIIIIIIIIIIIIIIIHFII86II6IICI1CI4I4*5*4B( @SRR019778.74 I331_2_FC3076HAAXX:1:1:1769:1745 length=45 GAAGTGATGTAGATGCCTATATGAATACAGGCACATATGGTTTCC +SRR019778.74 I331_2_FC3076HAAXX:1:1:1769:1745 length=45 IIIIDIIIIIIIIIIIII7IIIIG>I0E2II=+7/:+7DA04*'7 @SRR019778.75 I331_2_FC3076HAAXX:1:1:721:1248 length=45 GACAAGTTTAGGAAGCCTGTTATTTTACTACATGAAACATTCATA +SRR019778.75 I331_2_FC3076HAAXX:1:1:721:1248 length=45 IIEIIIIIIIIIIIIIIII>IIIIII>III9AI+75>76?84-2I @SRR019778.76 I331_2_FC3076HAAXX:1:1:1306:616 length=45 GTTAAGATAAATCACAGAGCACCAACCTCAAGCGATGGCAGCTGC +SRR019778.76 I331_2_FC3076HAAXX:1:1:1306:616 length=45 IIIIIIIIIIIIIIIIIIIIIIIIAIC=B6:57:274.2,+(+%& @SRR019778.77 I331_2_FC3076HAAXX:1:1:1474:1625 length=45 GTAAAGGACTTTCAGGACCTAATTTAAATGATCACTCAGGCTGAG +SRR019778.77 I331_2_FC3076HAAXX:1:1:1474:1625 length=45 IIIIIIIIIIIIIIIIIIII>9III@7GII3IIFIIA34'1FA)7 @SRR019778.78 I331_2_FC3076HAAXX:1:1:1866:942 length=45 GACAGATACTGCCTTACTATGCCTTACTATGTGCACATATACACA +SRR019778.78 I331_2_FC3076HAAXX:1:1:1866:942 length=45 IIIIIIIIIIIIIIIIII5IIIIII1II.I+:,C33,?/;,,)/) @SRR019778.79 I331_2_FC3076HAAXX:1:1:1803:1776 length=45 GTAATATATAAACAGCATAGATAATATCAGATAAAAGGACTTTCT +SRR019778.79 I331_2_FC3076HAAXX:1:1:1803:1776 length=45 IIIIIIIIIIIII:II8IDI;I9:I/=E;I>AA0132/29>=/15 @SRR019778.80 I331_2_FC3076HAAXX:1:1:716:927 length=45 GCAAGGTAGGGACACGGGGACTCAGACAGATGAACAGATTAGACT +SRR019778.80 I331_2_FC3076HAAXX:1:1:716:927 length=45 IIIIIIIIIIIIIIIIIIIACIGBI=?2I6DA8;3+;/5:/./+1 @SRR019778.81 I331_2_FC3076HAAXX:1:1:1294:71 length=45 GTAAACATGGAACACAACAAAAAAACTTCAAAAAGGCAAAACTAG +SRR019778.81 I331_2_FC3076HAAXX:1:1:1294:71 length=45 IIIIIIIIIIIIIDIIII93I@AE.HE7I632-,63?72/1476, @SRR019778.82 I331_2_FC3076HAAXX:1:1:1486:1393 length=45 GATTTAAACAATTACATCAAAATGCATTGATTCAATTGTTTTTTA +SRR019778.82 I331_2_FC3076HAAXX:1:1:1486:1393 length=45 IIIIIIIIIIIIIIIIIIIBIIIID9:I@;4@8*B0I4A77 @SRR019778.83 I331_2_FC3076HAAXX:1:1:1880:1764 length=45 GTAAAAATAACTACATTAACTGTGACAAGGGATACCCAAGAAGAT +SRR019778.83 I331_2_FC3076HAAXX:1:1:1880:1764 length=45 IIIIIIIIIIIIIIIIIIIIIIAI:I8:IHI.52IG4'/4-+2)0 @SRR019778.84 I331_2_FC3076HAAXX:1:1:1410:1972 length=45 GCATGTGGATATCCAGTTTTCCCATTTCTTGAAAAGTGTGTCCTT +SRR019778.84 I331_2_FC3076HAAXX:1:1:1410:1972 length=45 IIIIIIIIIIIIII/IIIIIIII7IIIIII;3.)1=<<8-D86G> @SRR019778.85 I331_2_FC3076HAAXX:1:1:1681:1342 length=45 GTAGAAATGACCTTAGCCCACTATACTAGAAACTTCGGGTTTGTG +SRR019778.85 I331_2_FC3076HAAXX:1:1:1681:1342 length=45 IIIIIIIIIIIIIIIIIIFDII3IFIII)712145+.5,1&3+ @SRR019778.87 I331_2_FC3076HAAXX:1:1:1395:1991 length=45 GTGCTATCTCATCATGGTTTTGATCTGTATTTCCCTGATAATGAG +SRR019778.87 I331_2_FC3076HAAXX:1:1:1395:1991 length=45 IIIIIIIIIIIII?IIIIIIII2IIIIF7IIIGCEI;+D&-B+** @SRR019778.88 I331_2_FC3076HAAXX:1:1:1332:1959 length=45 GATAGTAACTCCTGTCTTCCTACGGGGTTAAAAAAAGCCTTCTGC +SRR019778.88 I331_2_FC3076HAAXX:1:1:1332:1959 length=45 IIIIIIIIIIIIIIIIIIII3IIIIII=ID-2/+824<;4?A872 @SRR019778.89 I331_2_FC3076HAAXX:1:1:1304:77 length=45 GAAGAGAGAAGTCTGGGGGATCAGAGGCCACCAAAAGCAAAACAA +SRR019778.89 I331_2_FC3076HAAXX:1:1:1304:77 length=45 IIIIIIIIIIIIIIICIDI?D97957@;+839,))+5-2*')**) @SRR019778.90 I331_2_FC3076HAAXX:1:1:1478:1372 length=45 GTTAAAGGTGAAGAAGGAAGAGAAGTTCCTTCCATTTGGCAGATT +SRR019778.90 I331_2_FC3076HAAXX:1:1:1478:1372 length=45 IIIIIIIIIIIIIIIIIIIIII2AI3II>IB@50A:3<*5+@):2 @SRR019778.91 I331_2_FC3076HAAXX:1:1:1518:630 length=45 GTAGTGGTGAAAGTATTTATTTTTCCCTATATCTTTGTTAATTGC +SRR019778.91 I331_2_FC3076HAAXX:1:1:1518:630 length=45 IIIIIIIIIIIIICIIII@IIIIIIDFI?I1I9IHC$:B.+BI%) @SRR019778.92 I331_2_FC3076HAAXX:1:1:1486:1973 length=45 GAAACAGACAAAAGCCTTAGTAACCTGGGGCTCAGCAAAAGGCCA +SRR019778.92 I331_2_FC3076HAAXX:1:1:1486:1973 length=45 IIIIIIIIIIIIIIIIIIIIIH?IIIIIIIIII3IH-//(I??I3 @SRR019778.93 I331_2_FC3076HAAXX:1:1:1296:1435 length=45 GAGTTTTGAACTCAGGCTGTTCTGGCCTCAAAATTCATGCTCATT +SRR019778.93 I331_2_FC3076HAAXX:1:1:1296:1435 length=45 IIIIIIIIIIIIIIIIIIIII4@&;74C1)01/9I)'9&-7+(29 @SRR019778.94 I331_2_FC3076HAAXX:1:1:1502:1287 length=45 GCACAGATGAGCCAAGAAGGGTTCCAGTTCCCCTGCTTCATTATG +SRR019778.94 I331_2_FC3076HAAXX:1:1:1502:1287 length=45 IIIIIIIIIIIIII;'*B63'20,%6- @SRR019778.96 I331_2_FC3076HAAXX:1:1:1878:956 length=45 GATAATATACTATGGCTCTGCAAGATAGTACTTGGGGGAAACAAA +SRR019778.96 I331_2_FC3076HAAXX:1:1:1878:956 length=45 IIIIIIIIIIIIIIIIII(/:*--01+1.2.-$/+0&6#,2&(&4 @SRR019778.97 I331_2_FC3076HAAXX:1:1:608:1209 length=45 GAAATTTTCCTTCAATAAAGGGAGATTCTAATCTACATATTTTAA +SRR019778.97 I331_2_FC3076HAAXX:1:1:608:1209 length=45 IIIIIIIIIIIIIIIIHH4I=A masked.fa This will create a new FASTA file called "masked.fa" that replaces all masked regions with lowercase letters. (tantan also works on FASTQ-format, though it does not use the quality data.) * To mask proteins effectively, tantan needs to use different algorithm parameters than for nucleotides. You have to tell it when you have proteins, using the "-p" option:: tantan -p aaseqs.fa > masked.fa If you omit "-p" and the sequences look proteinaceous, tantan will print a warning message. Advanced usage -------------- * By default, tantan indicates repetitive regions with lowercase letters. You can make it replace repetitive letters with (say) "N" by using the "-x" option:: tantan -x N ntseqs.fa > masked.fa * By default, tantan does not preserve lowercase letters in the input sequences. You can tell it to preserve them by using the "-c" option. So the output will have the union of the lowercase in the input and the lowercase assigned by tantan:: tantan -c ntseqs.fa > masked.fa * tantan's masking rate is usually OK, but you can alter it by changing the "-r" parameter from its default value of 0.005. Higher values increase the amount of masking, and lower values decrease it. This increases the masking rate:: tantan -r 0.02 ntseqs.fa > masked.fa * Finally, to mask extremely AT-rich DNA, you should change tantan's scoring matrix. The "test" directory contains a matrix "atMask.mat" that works well for DNA with ~80% A+T, such as Plasmodium and Dictyostelium genomes. We recommend masking such DNA like this:: tantan -m atMask.mat -r 0.01 atrich.fa > masked.fa The preceding examples cover all of tantan's options that you should ever need. Recommendations for homology search ----------------------------------- 1) Mask *both* (sets of) sequences. 2) If for some reason you wish to mask only one (set of) sequence(s), increase "-r" to 0.02 (0.05 for AT-rich DNA). 3) For DNA-versus-protein alignment, increase "-r" for the proteins to 0.02. If for some reason you mask only one (set of) sequence(s), make sure it's the proteins. 4) Some alignment tools exclude lowercase from their initial seeding phase, but treat lowercase identically to uppercase during their subsequent extension phase. Unfortunately, this does not reliably prevent false homology predictions. It is OK to re-align without masking after homology has been decided: FASTA and LAST can do this. For more information, please read the tantan publication (see below). FAQ --- Why does tantan sometimes mask isolated bases? ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This happens when a region is borderline repetitive, and a single base creeps just over the threshold. Don't worry about it, it's not a problem (at least for tantan's aim of preventing false homology). Does tantan mask functional sequence? ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Of course. Proteins and protein-coding exons can contain simple repeats. Repeats can be functional. If we want to avoid false homology we have to mask them. Remember that tantan merely lowercases repeats, so it's easy to lift the masking after determining homology. Options ------- -p interpret the sequences as proteins -x letter to use for masking, instead of lowercase -c preserve uppercase/lowercase in non-masked regions -m file for letter-pair score matrix -r probability of a repeat starting per position -e probability of a repeat ending per position -w maximum tandem repeat period to consider -d probability decay per period (period-(i+1) / period-i) -i match score -j mismatch cost -a gap existence cost -b gap extension cost -s minimum repeat probability for masking -n minimum copy number, affects -f4 only -f output type: 0=masked sequence, 1=repeat probabilities, 2=repeat counts, 3=BED, 4=tandem repeats -h, --help show help message, then exit --version show version information, then exit Advanced issues --------------- When tantan masks tandem repeats, it tends to leave the first (left-most) repeat unit unmasked. This sometimes allows us to find homologs we would otherwise miss:: TGCAAGCTA TTAGGCTTAGGTCAGTGC ttaagcttaggtcagtgc AACATA ||| ||| | |||||||||||||||||| ||| |||||||||||||| ||| || TGCTAGCAA TTAGGCTTAGGTCAGTGC ttaggcttaggtcagtgc AACGTA However, there is a danger of non-equivalent repeat units being unmasked. This happens especially if we mask DNA on one strand but align it on the other strand:: TGCAAGCTA TTAGGCTTAGGTCAGTGC ttaagcttaggtcagtgc AACATA |||||||||||||||||| TGCTAGCAA ttaggcttaggtcagtgc TTAGGCTTAGGTCAGTGC AACGTA (My thanks to Junko Tsuji and Paul Horton for finding these issues.) Finding straightforward tandem repeats -------------------------------------- Option -f4 runs tantan in a different mode, where it finds straightforward tandem repeats only. (Technically, it uses a Viterbi algorithm instead of a Forward-Backward algorithm.) This is *not* recommended for avoiding false homologs! But it might be useful for studying tandem repeats. The output looks like this:: mySeq 14765 14780 6 2.5 GTCATG GTCATG,GTCATG,GTC mySeq 632362 632377 2 6 GC GC,GC,GC,GCt,GCT,GCT mySeq 1278353 1278369 3 6.5 TCA TCA,TCA,TCA,TC-,TC,TC,T mySeq 3616084 3616100 3 5.33333 TGG TGA,TGA,TGG,TGG,TGG,T The first 3 columns show the start and end coordinates of the repetitive region, in `BED `_ format. Column 4 shows the length of the repeating unit (which might vary due to insertions and deletions, so this column shows the most common length). Column 5 shows the number of repeat units. Column 6 shows the repeating unit (which again might vary, so this is just a representative). Column 7 shows the whole repeat: lowercase letters are insertions relative to the previous repeat unit, and dashes are deletions relative to the previous repeat unit. Miscellaneous ------------- tantan is distributed under the GNU General Public License, either version 3 of the License, or (at your option) any later version. For details, see COPYING.txt. If you use tantan in your research, please cite: "A new repeat-masking method enables specific detection of homologous sequences", MC Frith, Nucleic Acids Research 2011 39(4):e23. tantan's website is: http://www.cbrc.jp/tantan/ If you have any questions, comments, or problems concerning tantan, please email: tantan (ATmark) cbrc (dot) jp. tantan-22/Makefile0000644000000000000000000000101513406427735012732 0ustar rootrootCXXFLAGS = -O3 all: @cd src && ${MAKE} CXXFLAGS="${CXXFLAGS}" prefix = /usr/local exec_prefix = ${prefix} bindir = ${exec_prefix}/bin install: all mkdir -p ${bindir} cp src/tantan ${bindir} clean: @cd src && ${MAKE} clean README.html: README.txt rst2html README.txt > README.html log: hg log --style changelog > ChangeLog.txt distdir = tantan-`hg id -n` dist: README.html log @cd src && ${MAKE} version.hh rsync -rC --exclude tantan src test Makefile *.txt README.html ${distdir} zip -qrm ${distdir} ${distdir} tantan-22/src/0000755000000000000000000000000013406427735012064 5ustar rootroottantan-22/src/mcf_score_matrix.cc0000644000000000000000000001374513406427735015731 0ustar rootroot// Copyright 2010 Martin C. Frith #include "mcf_score_matrix.hh" #include // min, fill_n #include #include #include // setw #include #include #include namespace mcf { typedef std::runtime_error Error; // copied from NCBI BLAST: const char* ScoreMatrix::blosum62 = "\ A R N D C Q E G H I L K M F P S T W Y V B J Z X *\n\ A 4 -1 -2 -2 0 -1 -1 0 -2 -1 -1 -1 -1 -2 -1 1 0 -3 -2 0 -2 -1 -1 -1 -4\n\ R -1 5 0 -2 -3 1 0 -2 0 -3 -2 2 -1 -3 -2 -1 -1 -3 -2 -3 -1 -2 0 -1 -4\n\ N -2 0 6 1 -3 0 0 0 1 -3 -3 0 -2 -3 -2 1 0 -4 -2 -3 4 -3 0 -1 -4\n\ D -2 -2 1 6 -3 0 2 -1 -1 -3 -4 -1 -3 -3 -1 0 -1 -4 -3 -3 4 -3 1 -1 -4\n\ C 0 -3 -3 -3 9 -3 -4 -3 -3 -1 -1 -3 -1 -2 -3 -1 -1 -2 -2 -1 -3 -1 -3 -1 -4\n\ Q -1 1 0 0 -3 5 2 -2 0 -3 -2 1 0 -3 -1 0 -1 -2 -1 -2 0 -2 4 -1 -4\n\ E -1 0 0 2 -4 2 5 -2 0 -3 -3 1 -2 -3 -1 0 -1 -3 -2 -2 1 -3 4 -1 -4\n\ G 0 -2 0 -1 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 0 -2 -2 -3 -3 -1 -4 -2 -1 -4\n\ H -2 0 1 -1 -3 0 0 -2 8 -3 -3 -1 -2 -1 -2 -1 -2 -2 2 -3 0 -3 0 -1 -4\n\ I -1 -3 -3 -3 -1 -3 -3 -4 -3 4 2 -3 1 0 -3 -2 -1 -3 -1 3 -3 3 -3 -1 -4\n\ L -1 -2 -3 -4 -1 -2 -3 -4 -3 2 4 -2 2 0 -3 -2 -1 -2 -1 1 -4 3 -3 -1 -4\n\ K -1 2 0 -1 -3 1 1 -2 -1 -3 -2 5 -1 -3 -1 0 -1 -3 -2 -2 0 -3 1 -1 -4\n\ M -1 -1 -2 -3 -1 0 -2 -3 -2 1 2 -1 5 0 -2 -1 -1 -1 -1 1 -3 2 -1 -1 -4\n\ F -2 -3 -3 -3 -2 -3 -3 -3 -1 0 0 -3 0 6 -4 -2 -2 1 3 -1 -3 0 -3 -1 -4\n\ P -1 -2 -2 -1 -3 -1 -1 -2 -2 -3 -3 -1 -2 -4 7 -1 -1 -4 -3 -2 -2 -3 -1 -1 -4\n\ S 1 -1 1 0 -1 0 0 0 -1 -2 -2 0 -1 -2 -1 4 1 -3 -2 -2 0 -2 0 -1 -4\n\ T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 1 5 -2 -2 0 -1 -1 -1 -1 -4\n\ W -3 -3 -4 -4 -2 -2 -3 -2 -2 -3 -2 -3 -1 1 -4 -3 -2 11 2 -3 -4 -2 -2 -1 -4\n\ Y -2 -2 -2 -3 -2 -1 -2 -3 2 -1 -1 -2 -1 3 -3 -2 -2 2 7 -1 -3 -1 -2 -1 -4\n\ V 0 -3 -3 -3 -1 -2 -2 -3 -3 3 1 -2 1 -1 -2 -2 0 -3 -1 4 -3 2 -2 -1 -4\n\ B -2 -1 4 4 -3 0 1 -1 0 -3 -4 0 -3 -3 -2 0 -1 -4 -3 -3 4 -3 0 -1 -4\n\ J -1 -2 -3 -3 -1 -2 -3 -4 -3 3 3 -3 2 0 -3 -2 -1 -2 -1 2 -3 3 -3 -1 -4\n\ Z -1 0 0 1 -3 4 4 -2 0 -3 -3 1 -1 -3 -1 0 -1 -2 -2 -2 0 -3 4 -1 -4\n\ X -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 -4\n\ * -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 1\n\ "; void ScoreMatrix::initMatchMismatch(int matchScore, int mismatchCost, const std::string &letters) { rowLetters.assign(letters.begin(), letters.end()); colLetters.assign(letters.begin(), letters.end()); unsigned size = letters.size(); assert(size == letters.size()); scores.resize(size); for (unsigned i = 0; i < size; ++i) { scores[i].assign(size, -mismatchCost); scores[i][i] = matchScore; } } int ScoreMatrix::minScore() const { int m = std::numeric_limits::max(); for (unsigned i = 0; i < scores.size(); ++i) for (unsigned j = 0; j < scores[i].size(); ++j) m = std::min(m, scores[i][j]); return m; } static uchar indexOrDie(uchar letter, const uchar *letterToIndex, int tooBig) { uchar x = letterToIndex[letter]; if (x >= tooBig) throw Error(std::string("bad letter in score matrix: ") + static_cast(letter)); return x; } void ScoreMatrix::makeFastMatrix(int **fastMatrix, int fastMatrixSize, const uchar *letterToIndex, int defaultScore, bool isCaseSensitive) const { for (int i = 0; i < fastMatrixSize; ++i) std::fill_n(fastMatrix[i], fastMatrixSize, defaultScore); for (unsigned i = 0; i < rowLetters.size(); ++i) { for (unsigned j = 0; j < colLetters.size(); ++j) { uchar x = static_cast(rowLetters[i]); uchar y = static_cast(colLetters[j]); if (isCaseSensitive) { uchar a = indexOrDie(x, letterToIndex, fastMatrixSize); uchar b = indexOrDie(y, letterToIndex, fastMatrixSize); fastMatrix[a][b] = scores[i][j]; } else { uchar a = indexOrDie(std::toupper(x), letterToIndex, fastMatrixSize); uchar b = indexOrDie(std::tolower(x), letterToIndex, fastMatrixSize); uchar c = indexOrDie(std::toupper(y), letterToIndex, fastMatrixSize); uchar d = indexOrDie(std::tolower(y), letterToIndex, fastMatrixSize); fastMatrix[a][c] = scores[i][j]; fastMatrix[a][d] = scores[i][j]; fastMatrix[b][c] = scores[i][j]; fastMatrix[b][d] = scores[i][j]; } } } } std::istream &operator>>(std::istream &s, ScoreMatrix &m) { std::vector tmpRowLetters; std::vector tmpColLetters; std::vector< std::vector > tmpScores; std::string line; while (std::getline(s, line)) { std::istringstream iss(line); char c; if (!(iss >> c)) continue; // skip blank lines if (c == '#') continue; // skip comment lines if (tmpColLetters.empty()) { do { tmpColLetters.push_back(c); } while (iss >> c); } else { tmpRowLetters.push_back(c); tmpScores.resize(tmpScores.size() + 1); int score; while (iss >> score) { tmpScores.back().push_back(score); } if (tmpScores.back().size() != tmpColLetters.size()) s.setstate(std::ios::failbit); } } if (s.eof() && !s.bad() && !tmpScores.empty()) { s.clear(); tmpRowLetters.swap(m.rowLetters); tmpColLetters.swap(m.colLetters); tmpScores.swap(m.scores); } return s; } std::ostream &operator<<(std::ostream &s, const ScoreMatrix &m) { s << ' '; for (unsigned j = 0; j < m.colLetters.size(); ++j) s << ' ' << std::setw(2) << m.colLetters[j]; s << '\n'; for (unsigned i = 0; i < m.rowLetters.size(); ++i) { s << m.rowLetters[i]; for (unsigned j = 0; j < m.colLetters.size(); ++j) { s << ' ' << std::setw(2) << m.scores[i][j]; } s << '\n'; } return s; } } tantan-22/src/mcf_tantan_options.cc0000644000000000000000000001264613406427735016271 0ustar rootroot// Copyright 2010 Martin C. Frith #include "mcf_tantan_options.hh" #include "mcf_util.hh" #include #include // EXIT_SUCCESS #include #include namespace mcf { typedef std::runtime_error Error; static void badopt(char opt, const char *arg) { throw Error(std::string("bad option value: -") + opt + " " + arg); } static void writeAndQuit(const std::string &text) { std::cout << text; throw EXIT_SUCCESS; } static int myGetopt(int argc, char **argv, const char *optstring, const std::string &help, const std::string &version) { if (optind < argc) { std::string nextarg = argv[optind]; if (nextarg == "--help") writeAndQuit(help); if (nextarg == "--version") writeAndQuit(version); } return getopt(argc, argv, optstring); } std::istream &operator>>(std::istream &s, TantanOptions::OutputType &x) { int i = 0; s >> i; if (i < 0 || i > 4) s.setstate(std::ios::failbit); if (s) x = static_cast(i); return s; } TantanOptions::TantanOptions() : isProtein(false), maskSymbol(0), isPreserveLowercase(false), scoreMatrixFileName(0), repeatProb(0.005), repeatEndProb(0.05), maxCycleLength(-1), // depends on isProtein repeatOffsetProbDecay(0.9), matchScore(0), mismatchCost(0), gapExistenceCost(0), gapExtensionCost(-1), // means: no gaps minMaskProb(0.5), minCopyNumber(2.0), outputType(maskOut), indexOfFirstNonOptionArgument(-1) {} void TantanOptions::fromArgs(int argc, char **argv) { std::string help = "\ Usage: tantan [options] fasta-sequence-file(s)\n\ Find simple repeats in sequences\n\ \n\ Options (default settings):\n\ -p interpret the sequences as proteins\n\ -x letter to use for masking, instead of lowercase\n\ -c preserve uppercase/lowercase in non-masked regions\n\ -m file for letter-pair score matrix\n\ -r probability of a repeat starting per position (" + stringify(repeatProb) + ")\n\ -e probability of a repeat ending per position (" + stringify(repeatEndProb) + ")\n\ -w maximum tandem repeat period to consider (100, but -p selects 50)\n\ -d probability decay per period (" + stringify(repeatOffsetProbDecay) + ")\n\ -i match score (BLOSUM62 if -p, else 2 if -f4, else 1)\n\ -j mismatch cost (BLOSUM62 if -p, else 7 if -f4, else 1)\n\ -a gap existence cost (" + stringify(gapExistenceCost) + ")\n\ -b gap extension cost (7 if -f4, else infinity)\n\ -s minimum repeat probability for masking (" + stringify(minMaskProb) + ")\n\ -n minimum copy number, affects -f4 only (" + stringify(minCopyNumber) + ")\n\ -f output type: 0=masked sequence, 1=repeat probabilities,\n\ 2=repeat counts, 3=BED, 4=tandem repeats (" + stringify(outputType) + ")\n\ -h, --help show help message, then exit\n\ --version show version information, then exit\n\ \n\ Report bugs to: tantan@cbrc.jp\n\ Home page: http://www.cbrc.jp/tantan/\n\ "; // -k for transition cost? std::string version = "tantan " #include "version.hh" "\n"; const char *optstring = "px:cm:r:e:w:d:i:j:a:b:s:n:f:h"; int i; while ((i = myGetopt(argc, argv, optstring, help, version)) != -1) { char c = static_cast(i); switch (c) { case 'p': isProtein = true; break; case 'x': unstringify(maskSymbol, optarg); break; case 'c': isPreserveLowercase = true; break; case 'm': scoreMatrixFileName = optarg; break; case 'r': unstringify(repeatProb, optarg); if (repeatProb < 0 || repeatProb >= 1) badopt(c, optarg); break; case 'e': unstringify(repeatEndProb, optarg); if (repeatEndProb < 0 || repeatEndProb > 1) badopt(c, optarg); break; case 'w': unstringify(maxCycleLength, optarg); if (maxCycleLength <= 0 ) badopt(c, optarg); break; case 'd': unstringify(repeatOffsetProbDecay, optarg); if (repeatOffsetProbDecay <= 0 || repeatOffsetProbDecay > 1) badopt(c, optarg); break; case 'i': unstringify(matchScore, optarg); if (matchScore <= 0) badopt(c, optarg); break; case 'j': unstringify(mismatchCost, optarg); if (mismatchCost <= 0) badopt(c, optarg); break; case 'a': unstringify(gapExistenceCost, optarg); break; case 'b': unstringify(gapExtensionCost, optarg); if (gapExtensionCost <= 0) badopt(c, optarg); break; case 's': unstringify(minMaskProb, optarg); // don't bother checking for stupid values? break; case 'n': unstringify(minCopyNumber, optarg); break; case 'f': unstringify(outputType, optarg); break; case 'h': writeAndQuit(help); case '?': throw Error("bad option"); } } if (gapExtensionCost < 0 && outputType == repOut) gapExtensionCost = 7; if (gapExtensionCost > 0 && gapExistenceCost + gapExtensionCost <= 0) throw Error("gap existence + extension cost is too low"); if (maxCycleLength < 0) maxCycleLength = (isProtein ? 50 : 100); if (!isProtein || matchScore || mismatchCost) { if (matchScore == 0) matchScore = (outputType == repOut ? 2 : 1); if (mismatchCost == 0) mismatchCost = (outputType == repOut ? 7 : 1); } indexOfFirstNonOptionArgument = optind; } } tantan-22/src/tantan_app.cc0000644000000000000000000003357413406427735014534 0ustar rootroot// Copyright 2010 Martin C. Frith // Mask simple regions (low complexity & short-period tandem repeats) // in biological sequences. #include "mcf_alphabet.hh" #include "mcf_fasta_sequence.hh" #include "mcf_score_matrix.hh" #include "mcf_tantan_options.hh" #include "mcf_util.hh" #include "tantan.hh" #include "tantan_repeat_finder.hh" #include "LambdaCalculator.hh" #include // copy, fill_n #include #include #include // EXIT_SUCCESS, EXIT_FAILURE #include // exception #include #include #include // bad_alloc #include #define BEG(v) ((v).empty() ? 0 : &(v).front()) #define END(v) ((v).empty() ? 0 : &(v).back() + 1) typedef std::runtime_error Error; using namespace mcf; // move this function to a reusable file? bool isDubiousDna(const uchar *beg, const uchar *end) { int badLetters = 0; if (end - beg > 100) end = beg + 100; // just check the first 100 letters while (beg < end) { if (!strchr("AaCcGgTtNnUu", *beg)) { ++badLetters; if (badLetters > 10) return true; } ++beg; } return false; } namespace { TantanOptions options; Alphabet alphabet; tantan::RepeatFinder repeatFinder; enum { scoreMatrixSize = 64 }; int fastMatrix[scoreMatrixSize][scoreMatrixSize]; int *fastMatrixPointers[scoreMatrixSize]; double probMatrix[scoreMatrixSize][scoreMatrixSize]; double *probMatrixPointers[scoreMatrixSize]; double firstGapProb; double otherGapProb; uchar hardMaskTable[Alphabet::capacity]; const uchar *maskTable; std::vector transitionCounts; double transitionTotal; } void initAlphabet() { if (options.isProtein) alphabet.fromString(Alphabet::protein); else alphabet.fromString(Alphabet::dna); } void initScoresAndProbabilities() { std::copy(fastMatrix, fastMatrix + scoreMatrixSize, fastMatrixPointers); std::copy(probMatrix, probMatrix + scoreMatrixSize, probMatrixPointers); ScoreMatrix scoreMatrix; if (options.scoreMatrixFileName) { unfilify(scoreMatrix, options.scoreMatrixFileName); } else if (options.isProtein) { if (options.matchScore && options.mismatchCost) { scoreMatrix.initMatchMismatch(options.matchScore, options.mismatchCost, Alphabet::protein); } else { unstringify(scoreMatrix, ScoreMatrix::blosum62); } } else { scoreMatrix.initMatchMismatch(options.matchScore, options.mismatchCost, "ACGTU"); // allow for RNA } scoreMatrix.makeFastMatrix(fastMatrixPointers, scoreMatrixSize, alphabet.lettersToNumbers, scoreMatrix.minScore(), false); cbrc::LambdaCalculator matCalc; matCalc.calculate(fastMatrixPointers, alphabet.size); if (matCalc.isBad()) throw Error("can't calculate probabilities for this score matrix"); double matrixLambda = matCalc.lambda(); for (int i = 0; i < scoreMatrixSize; ++i) { for (int j = 0; j < scoreMatrixSize; ++j) { double x = matrixLambda * fastMatrix[i][j]; if (options.outputType != options.repOut) x = std::exp(x); probMatrix[i][j] = x; } } if (options.gapExtensionCost > 0) { int firstGapCost = options.gapExistenceCost + options.gapExtensionCost; firstGapProb = std::exp(-matrixLambda * firstGapCost); otherGapProb = std::exp(-matrixLambda * options.gapExtensionCost); // the gap existence cost includes the gap ending cost: firstGapProb /= (1 - otherGapProb); // XXX check if firstGapProb is too high } repeatFinder.init(options.maxCycleLength, probMatrixPointers, options.repeatProb, options.repeatEndProb, options.repeatOffsetProbDecay, firstGapProb, otherGapProb); //std::cerr << "lambda: " << matrixLambda << "\n"; //std::cerr << "firstGapProb: " << firstGapProb << "\n"; //std::cerr << "otherGapProb: " << otherGapProb << "\n"; } void initMaskTable() { uchar maskSymbol = static_cast(options.maskSymbol); uchar maskNumber = alphabet.lettersToNumbers[maskSymbol]; std::fill_n(hardMaskTable, Alphabet::capacity, maskNumber); if (maskSymbol == 0) maskTable = alphabet.numbersToLowercase; else maskTable = hardMaskTable; } std::string firstWord(const std::string &s) { std::string word; std::istringstream iss(s); iss >> word; return word; // might be empty } void writeBedLine(const std::string &seqName, const float *origin, const float *beg, const float *end, std::ostream &out) { out << seqName << '\t' << (beg - origin) << '\t' << (end - origin) << '\n'; } void writeBed(const float *probBeg, const float *probEnd, const std::string &seqName, std::ostream &output) { if (seqName.empty()) throw Error("missing sequence name"); const float *maskBeg = 0; // pointer to start of masked tract for (const float *i = probBeg; i < probEnd; ++i) { if (*i >= options.minMaskProb) { // this position is masked if (maskBeg == 0) maskBeg = i; } else { // this position is not masked if (maskBeg) writeBedLine(seqName, probBeg, maskBeg, i, output); maskBeg = 0; } } if (maskBeg) writeBedLine(seqName, probBeg, maskBeg, probEnd, output); } void storeSequence(const uchar *beg, const uchar *end, std::string &out) { out.clear(); for (const uchar *i = beg; i < end; ++i) { out.push_back(std::toupper(alphabet.numbersToLetters[*i])); } } struct RepeatUnit { const uchar *beg; int len; }; bool less(const RepeatUnit &x, const RepeatUnit &y) { if (x.len != y.len) return x.len < y.len; int c = memcmp(x.beg, y.beg, x.len); if (c != 0) return c < 0; return x.beg > y.beg; } int mainLen(const std::vector &repUnits) { int bestLen; size_t bestCount = 0; size_t count = 0; for (size_t i = 0; i < repUnits.size(); ++i) { if (i && repUnits[i].len > repUnits[i - 1].len) { count = 0; } ++count; if (count > bestCount) { bestCount = count; bestLen = repUnits[i].len; } } return bestLen; } const uchar *mainBeg(const std::vector &repUnits, int len) { const uchar *bestBeg; size_t bestCount = 0; size_t count = 0; for (size_t i = 0; i < repUnits.size(); ++i) { if (repUnits[i].len != len) continue; if (count && memcmp(repUnits[i - 1].beg, repUnits[i].beg, len) != 0) { count = 0; } ++count; if (count < bestCount) continue; if (count > bestCount || repUnits[i].beg < bestBeg) { bestCount = count; bestBeg = repUnits[i].beg; } } return bestBeg; } void writeRepeat(const FastaSequence &f, const uchar *repBeg, const uchar *repEnd, const std::string &repText, std::vector &repUnits, const uchar *commaPos, int finalOffset) { double repeatCount = count(repText.begin(), repText.end(), ','); double copyNumber = repeatCount + (repEnd - commaPos) * 1.0 / finalOffset; if (copyNumber < options.minCopyNumber) return; sort(repUnits.begin(), repUnits.end(), less); int bestLen = mainLen(repUnits); const uchar *bestBeg = mainBeg(repUnits, bestLen); const uchar *beg = BEG(f.sequence); std::cout << firstWord(f.title) << '\t' << (repBeg - beg) << '\t' << (repEnd - beg) << '\t' << bestLen << '\t' << copyNumber << '\t'; for (int i = 0; i < bestLen; ++i) { char c = std::toupper(alphabet.numbersToLetters[bestBeg[i]]); std::cout << c; } std::cout << '\t'; std::cout << repText << '\n'; } void findRepeatsInOneSequence(const FastaSequence &f) { const uchar *beg = BEG(f.sequence); const uchar *end = END(f.sequence); repeatFinder.calcBestPathScore(beg, end); std::vector repUnits; std::string repText; const uchar *repBeg; const uchar *commaPos; int state = 0; for (const uchar *seqPtr = beg; seqPtr < end; ++seqPtr) { int newState = repeatFinder.nextState(); if (newState == 0) { if (state > 0) { writeRepeat(f, repBeg, seqPtr, repText, repUnits, commaPos, state); } } else if (newState <= options.maxCycleLength) { if (state == 0) { repUnits.clear(); repBeg = seqPtr - newState; storeSequence(repBeg, seqPtr, repText); commaPos = repBeg; } else if (state <= options.maxCycleLength) { for (int i = state; i > newState; --i) { if (seqPtr - commaPos >= i) { repText.push_back(','); commaPos = seqPtr; } repText.push_back('-'); } } RepeatUnit unit = {seqPtr - newState, newState}; repUnits.push_back(unit); if (seqPtr - commaPos >= newState) { repText.push_back(','); commaPos = seqPtr; } repText.push_back(std::toupper(alphabet.numbersToLetters[*seqPtr])); } else { repText.push_back(std::tolower(alphabet.numbersToLetters[*seqPtr])); } state = newState; } if (state > 0) { writeRepeat(f, repBeg, end, repText, repUnits, commaPos, state); } } void processOneSequence(FastaSequence &f, std::ostream &output) { uchar *beg = BEG(f.sequence); uchar *end = END(f.sequence); alphabet.encodeInPlace(beg, end); if (options.outputType == options.maskOut) { tantan::maskSequences(beg, end, options.maxCycleLength, probMatrixPointers, options.repeatProb, options.repeatEndProb, options.repeatOffsetProbDecay, firstGapProb, otherGapProb, options.minMaskProb, maskTable); alphabet.decodeInPlace(beg, end); output << f; } else if (options.outputType == options.countOut) { tantan::countTransitions(beg, end, options.maxCycleLength, probMatrixPointers, options.repeatProb, options.repeatEndProb, options.repeatOffsetProbDecay, firstGapProb, otherGapProb, BEG(transitionCounts)); double sequenceLength = static_cast(f.sequence.size()); transitionTotal += sequenceLength + 1; } else if (options.outputType == options.repOut) { findRepeatsInOneSequence(f); } else { std::vector probabilities(end - beg); float *probBeg = BEG(probabilities); float *probEnd = END(probabilities); tantan::getProbabilities(beg, end, options.maxCycleLength, probMatrixPointers, options.repeatProb, options.repeatEndProb, options.repeatOffsetProbDecay, firstGapProb, otherGapProb, probBeg); if (options.outputType == options.probOut) { output << '>' << f.title << '\n'; for (float *i = probBeg; i < probEnd; ++i) output << *i << '\n'; } else { writeBed(probBeg, probEnd, firstWord(f.title), output); } } } void processOneFile(std::istream &input, std::ostream &output) { bool isFirstSequence = true; // This code strives to minimize memory usage. The sequence-reading // operation does not overwrite the sequence until it finishes // reading successfully. So, we don't want to overwrite a large, // old sequence. Hence, we make a brand-new FastaSequence each time // through the loop. while (true) { FastaSequence f; if (!(input >> f)) break; if (isFirstSequence && !options.isProtein && isDubiousDna(BEG(f.sequence), END(f.sequence))) std::cerr << "tantan: that's some funny-lookin DNA\n"; processOneSequence(f, output); isFirstSequence = false; } } void writeCounts(std::ostream &output) { double bg2bg = transitionCounts[0]; output << "#period" << '\t' << "estimated number of tracts" << '\n'; double repeatCountSum = 0; double weightedSum = 0; for (int i = 1; i <= options.maxCycleLength; ++i) { assert(transitionCounts[i] >= 0); output << i << '\t' << transitionCounts[i] << '\n'; repeatCountSum += transitionCounts[i]; weightedSum += i * transitionCounts[i]; } output << "# estimated total number of repetitive tracts: " << repeatCountSum << '\n'; output << "# estimated number of background-to-background transitions: " << bg2bg << '\n'; output << "# total number of transitions (#letters + #sequences): " << transitionTotal << '\n'; double probDecay = 1 - repeatCountSum / weightedSum; output << "# best-fit probability decay per period: " << probDecay << '\n'; double repeatProb = repeatCountSum / (repeatCountSum + bg2bg); output << "# best-fit probability of a repeat starting per position: " << repeatProb << '\n'; if (firstGapProb > 0) return; // The remaining calculations are correct only if there are no gaps. // If repeatProb and repeatEndProb are fixed to be the same value, // and there are no gaps: // repeatProb = 2 * repeatCountSum / transitionTotal; double fg2fg = transitionTotal - bg2bg - 2 * repeatCountSum; double repeatEndProb = repeatCountSum / (repeatCountSum + fg2fg); output << "# best-fit probability of a repeat ending per position: " << repeatEndProb << '\n'; } int main(int argc, char **argv) try { options.fromArgs(argc, argv); initAlphabet(); initScoresAndProbabilities(); initMaskTable(); // do this after initMaskTable, so that the mask symbol can be lowercase: if (!options.isPreserveLowercase) alphabet.makeCaseInsensitive(); if (options.outputType == options.countOut) transitionCounts.resize(options.maxCycleLength + 1); std::ostream &output = std::cout; if (options.outputType == options.probOut) output.precision(3); if (options.indexOfFirstNonOptionArgument == argc) processOneFile(std::cin, output); for (int i = options.indexOfFirstNonOptionArgument; i < argc; ++i) { std::ifstream ifs; std::istream &input = openIn(argv[i], ifs); processOneFile(input, output); } if (options.outputType == options.countOut) writeCounts(output); return EXIT_SUCCESS; } catch( const std::bad_alloc& e ) { // bad_alloc::what() may be unfriendly std::cerr << "tantan: out of memory\n"; return EXIT_FAILURE; } catch (const std::exception &e) { std::cerr << "tantan: " << e.what() << '\n'; return EXIT_FAILURE; } catch (int i) { return i; } tantan-22/src/CA_code/0000755000000000000000000000000013406427735013341 5ustar rootroottantan-22/src/mcf_util.cc0000644000000000000000000000105713406427735014200 0ustar rootroot// Copyright 2010 Martin C. Frith #include "mcf_util.hh" #include namespace mcf { std::istream &openIn(const std::string &fileName, std::ifstream &ifs) { if (fileName == "-") return std::cin; ifs.open(fileName.c_str()); if (!ifs) throw std::runtime_error("can't open file: " + fileName); return ifs; } std::ostream &openOut(const std::string &fileName, std::ofstream &ofs) { if (fileName == "-") return std::cout; ofs.open(fileName.c_str()); if (!ofs) throw std::runtime_error("can't open file: " + fileName); return ofs; } } tantan-22/src/tantan.cc0000644000000000000000000003371113406427735013665 0ustar rootroot// Copyright 2010 Martin C. Frith #include "tantan.hh" #include // fill, max #include #include // pow, abs #include // cerr #include // accumulate #include #define BEG(v) ((v).empty() ? 0 : &(v).front()) #define END(v) ((v).empty() ? 0 : &(v).back() + 1) namespace tantan { void multiplyAll(std::vector &v, double factor) { for (std::vector::iterator i = v.begin(); i < v.end(); ++i) *i *= factor; } double firstRepeatOffsetProb(double probMult, int maxRepeatOffset) { if (probMult < 1 || probMult > 1) { return (1 - probMult) / (1 - std::pow(probMult, maxRepeatOffset)); } return 1.0 / maxRepeatOffset; } void checkForwardAndBackwardTotals(double fTot, double bTot) { double x = std::abs(fTot); double y = std::abs(bTot); // ??? Is 1e6 suitable here ??? if (std::abs(fTot - bTot) > std::max(x, y) / 1e6) std::cerr << "tantan: warning: possible numeric inaccuracy\n" << "tantan: forward algorithm total: " << fTot << "\n" << "tantan: backward algorithm total: " << bTot << "\n"; } struct Tantan { enum { scaleStepSize = 16 }; const uchar *seqBeg; // start of the sequence const uchar *seqEnd; // end of the sequence const uchar *seqPtr; // current position in the sequence int maxRepeatOffset; const const_double_ptr *likelihoodRatioMatrix; double b2b; // transition probability from background to background double f2b; // transition probability from foreground to background double g2g; // transition probability from gap/indel to gap/indel //double f2g; // transition probability from foreground to gap/indel //double g2f; // transition probability from gap/indel to foreground double oneGapProb; // f2g * g2f double endGapProb; // f2g * 1 double f2f0; // foreground to foreground, if there are 0 indel transitions double f2f1; // foreground to foreground, if there is 1 indel transition double f2f2; // foreground to foreground, if there are 2 indel transitions double b2fDecay; double b2fGrowth; double b2fFirst; // background state to first foreground state double b2fLast; // background state to last foreground state double backgroundProb; std::vector b2fProbs; // background state to each foreground state std::vector foregroundProbs; std::vector insertionProbs; std::vector scaleFactors; Tantan(const uchar *seqBeg, const uchar *seqEnd, int maxRepeatOffset, const const_double_ptr *likelihoodRatioMatrix, double repeatProb, double repeatEndProb, double repeatOffsetProbDecay, double firstGapProb, double otherGapProb) { assert(maxRepeatOffset > 0); assert(repeatProb >= 0 && repeatProb < 1); // (if repeatProb==1, then any sequence is impossible) assert(repeatEndProb >= 0 && repeatEndProb <= 1); assert(repeatOffsetProbDecay > 0 && repeatOffsetProbDecay <= 1); assert(otherGapProb >= 0 && otherGapProb <= 1); assert(firstGapProb >= 0); assert(repeatEndProb + firstGapProb * 2 <= 1); this->seqBeg = seqBeg; this->seqEnd = seqEnd; this->seqPtr = seqBeg; this->maxRepeatOffset = maxRepeatOffset; this->likelihoodRatioMatrix = likelihoodRatioMatrix; b2b = 1 - repeatProb; f2b = repeatEndProb; g2g = otherGapProb; //f2g = firstGapProb; //g2f = 1 - otherGapProb; oneGapProb = firstGapProb * (1 - otherGapProb); endGapProb = firstGapProb * (maxRepeatOffset > 1); f2f0 = 1 - repeatEndProb; f2f1 = 1 - repeatEndProb - firstGapProb; f2f2 = 1 - repeatEndProb - firstGapProb * 2; b2fDecay = repeatOffsetProbDecay; b2fGrowth = 1 / repeatOffsetProbDecay; b2fFirst = repeatProb * firstRepeatOffsetProb(b2fDecay, maxRepeatOffset); b2fLast = repeatProb * firstRepeatOffsetProb(b2fGrowth, maxRepeatOffset); b2fProbs.resize(maxRepeatOffset); foregroundProbs.resize(maxRepeatOffset); insertionProbs.resize(maxRepeatOffset - 1); double p = b2fFirst; for (int i = 0; i < maxRepeatOffset; ++i) { b2fProbs[i] = p; p *= b2fDecay; } scaleFactors.resize((seqEnd - seqBeg) / scaleStepSize); } void initializeForwardAlgorithm() { backgroundProb = 1.0; std::fill(foregroundProbs.begin(), foregroundProbs.end(), 0.0); std::fill(insertionProbs.begin(), insertionProbs.end(), 0.0); } double forwardTotal() { double fromForeground = std::accumulate(foregroundProbs.begin(), foregroundProbs.end(), 0.0); double total = backgroundProb * b2b + fromForeground * f2b; assert(total > 0); return total; } void initializeBackwardAlgorithm() { backgroundProb = b2b; std::fill(foregroundProbs.begin(), foregroundProbs.end(), f2b); std::fill(insertionProbs.begin(), insertionProbs.end(), 0.0); } double backwardTotal() { assert(backgroundProb > 0); return backgroundProb; } void calcForwardTransitionProbsWithGaps() { double fromBackground = backgroundProb * b2fLast; double *foregroundPtr = &foregroundProbs.back(); double f = *foregroundPtr; double fromForeground = f; double *insertionPtr = &insertionProbs.back(); double i = *insertionPtr; *foregroundPtr = fromBackground + f * f2f1 + i * endGapProb; double d = f; --foregroundPtr; fromBackground *= b2fGrowth; while (foregroundPtr > &foregroundProbs.front()) { f = *foregroundPtr; fromForeground += f; i = *(insertionPtr - 1); *foregroundPtr = fromBackground + f * f2f2 + (i + d) * oneGapProb; *insertionPtr = f + i * g2g; d = f + d * g2g; --foregroundPtr; --insertionPtr; fromBackground *= b2fGrowth; } f = *foregroundPtr; fromForeground += f; *foregroundPtr = fromBackground + f * f2f1 + d * endGapProb; *insertionPtr = f; backgroundProb = backgroundProb * b2b + fromForeground * f2b; } void calcBackwardTransitionProbsWithGaps() { double toBackground = f2b * backgroundProb; double *foregroundPtr = &foregroundProbs.front(); double f = *foregroundPtr; double toForeground = f; double *insertionPtr = &insertionProbs.front(); double i = *insertionPtr; *foregroundPtr = toBackground + f2f1 * f + i; double d = endGapProb * f; ++foregroundPtr; toForeground *= b2fGrowth; while (foregroundPtr < &foregroundProbs.back()) { f = *foregroundPtr; toForeground += f; i = *(insertionPtr + 1); *foregroundPtr = toBackground + f2f2 * f + (i + d); double oneGapProb_f = oneGapProb * f; *insertionPtr = oneGapProb_f + g2g * i; d = oneGapProb_f + g2g * d; ++foregroundPtr; ++insertionPtr; toForeground *= b2fGrowth; } f = *foregroundPtr; toForeground += f; *foregroundPtr = toBackground + f2f1 * f + d; *insertionPtr = endGapProb * f; backgroundProb = b2b * backgroundProb + b2fLast * toForeground; } void calcForwardTransitionProbs() { if (endGapProb > 0) return calcForwardTransitionProbsWithGaps(); double b = backgroundProb; double fromForeground = 0; double *foregroundBeg = BEG(foregroundProbs); for (int i = 0; i < maxRepeatOffset; ++i) { double f = foregroundBeg[i]; fromForeground += f; foregroundBeg[i] = b * b2fProbs[i] + f * f2f0; } backgroundProb = b * b2b + fromForeground * f2b; } void calcBackwardTransitionProbs() { if (endGapProb > 0) return calcBackwardTransitionProbsWithGaps(); double toBackground = f2b * backgroundProb; double toForeground = 0; double *foregroundBeg = BEG(foregroundProbs); for (int i = 0; i < maxRepeatOffset; ++i) { double f = foregroundBeg[i]; toForeground += b2fProbs[i] * f; foregroundBeg[i] = toBackground + f2f0 * f; } backgroundProb = b2b * backgroundProb + toForeground; } void addEndCounts(double forwardProb, double totalProb, double *transitionCounts) { double toEnd = forwardProb * b2b / totalProb; transitionCounts[0] += toEnd; } void addTransitionCounts(double forwardProb, double totalProb, double *transitionCounts) { double toBg = forwardProb * b2b / totalProb; double toFg = forwardProb * b2fFirst / totalProb; transitionCounts[0] += backgroundProb * toBg; for (double *i = BEG(foregroundProbs); i < END(foregroundProbs); ++i) { ++transitionCounts; *transitionCounts += *i * toFg; toFg *= b2fDecay; } } bool isNearSeqBeg() { return seqPtr - seqBeg < maxRepeatOffset; } const uchar *seqFurthestBack() { return isNearSeqBeg() ? seqBeg : seqPtr - maxRepeatOffset; } void calcEmissionProbs() { const double *lrRow = likelihoodRatioMatrix[*seqPtr]; const uchar *seqStop = seqFurthestBack(); double *foregroundPtr = BEG(foregroundProbs); const uchar *offsetPtr = seqPtr; while (offsetPtr > seqStop) { --offsetPtr; *foregroundPtr *= lrRow[*offsetPtr]; ++foregroundPtr; } while (foregroundPtr < END(foregroundProbs)) { *foregroundPtr *= 0; ++foregroundPtr; } } void rescale(double scale) { backgroundProb *= scale; multiplyAll(foregroundProbs, scale); multiplyAll(insertionProbs, scale); } void rescaleForward() { if ((seqPtr - seqBeg) % scaleStepSize == scaleStepSize - 1) { assert(backgroundProb > 0); double scale = 1 / backgroundProb; scaleFactors[(seqPtr - seqBeg) / scaleStepSize] = scale; rescale(scale); } } void rescaleBackward() { if ((seqPtr - seqBeg) % scaleStepSize == scaleStepSize - 1) { double scale = scaleFactors[(seqPtr - seqBeg) / scaleStepSize]; rescale(scale); } } void calcRepeatProbs(float *letterProbs) { initializeForwardAlgorithm(); while (seqPtr < seqEnd) { calcForwardTransitionProbs(); calcEmissionProbs(); rescaleForward(); *letterProbs = static_cast(backgroundProb); ++letterProbs; ++seqPtr; } double z = forwardTotal(); initializeBackwardAlgorithm(); while (seqPtr > seqBeg) { --seqPtr; --letterProbs; double nonRepeatProb = *letterProbs * backgroundProb / z; // Convert nonRepeatProb to a float, so that it is more likely // to be exactly 1 when it should be, e.g. for the 1st letter of // a sequence: *letterProbs = 1 - static_cast(nonRepeatProb); rescaleBackward(); calcEmissionProbs(); calcBackwardTransitionProbs(); } double z2 = backwardTotal(); checkForwardAndBackwardTotals(z, z2); } void countTransitions(double *transitionCounts) { std::vector p(seqEnd - seqBeg); float *letterProbs = BEG(p); initializeForwardAlgorithm(); while (seqPtr < seqEnd) { *letterProbs = static_cast(backgroundProb); calcForwardTransitionProbs(); calcEmissionProbs(); rescaleForward(); ++letterProbs; ++seqPtr; } double z = forwardTotal(); addEndCounts(backgroundProb, z, transitionCounts); initializeBackwardAlgorithm(); while (seqPtr > seqBeg) { --seqPtr; --letterProbs; rescaleBackward(); calcEmissionProbs(); addTransitionCounts(*letterProbs, z, transitionCounts); calcBackwardTransitionProbs(); } double z2 = backwardTotal(); checkForwardAndBackwardTotals(z, z2); } }; void maskSequences(uchar *seqBeg, uchar *seqEnd, int maxRepeatOffset, const const_double_ptr *likelihoodRatioMatrix, double repeatProb, double repeatEndProb, double repeatOffsetProbDecay, double firstGapProb, double otherGapProb, double minMaskProb, const uchar *maskTable) { std::vector p(seqEnd - seqBeg); float *probabilities = BEG(p); getProbabilities(seqBeg, seqEnd, maxRepeatOffset, likelihoodRatioMatrix, repeatProb, repeatEndProb, repeatOffsetProbDecay, firstGapProb, otherGapProb, probabilities); maskProbableLetters(seqBeg, seqEnd, probabilities, minMaskProb, maskTable); } void getProbabilities(const uchar *seqBeg, const uchar *seqEnd, int maxRepeatOffset, const const_double_ptr *likelihoodRatioMatrix, double repeatProb, double repeatEndProb, double repeatOffsetProbDecay, double firstGapProb, double otherGapProb, float *probabilities) { Tantan tantan(seqBeg, seqEnd, maxRepeatOffset, likelihoodRatioMatrix, repeatProb, repeatEndProb, repeatOffsetProbDecay, firstGapProb, otherGapProb); tantan.calcRepeatProbs(probabilities); } void maskProbableLetters(uchar *seqBeg, uchar *seqEnd, const float *probabilities, double minMaskProb, const uchar *maskTable) { while (seqBeg < seqEnd) { if (*probabilities >= minMaskProb) *seqBeg = maskTable[*seqBeg]; ++probabilities; ++seqBeg; } } void countTransitions(const uchar *seqBeg, const uchar *seqEnd, int maxRepeatOffset, const const_double_ptr *likelihoodRatioMatrix, double repeatProb, double repeatEndProb, double repeatOffsetProbDecay, double firstGapProb, double otherGapProb, double *transitionCounts) { Tantan tantan(seqBeg, seqEnd, maxRepeatOffset, likelihoodRatioMatrix, repeatProb, repeatEndProb, repeatOffsetProbDecay, firstGapProb, otherGapProb); tantan.countTransitions(transitionCounts); } } tantan-22/src/Makefile0000644000000000000000000000057013406427735013526 0ustar rootrootCXXFLAGS = -O3 -Wall -W -Wcast-qual -Wswitch-enum -Wundef \ -Wcast-align -Wold-style-cast # -Wconversion all: tantan tantan: *.cc *.hh version.hh Makefile $(CXX) $(CPPFLAGS) $(CXXFLAGS) $(LDFLAGS) -o $@ *.cc clean: rm -f tantan VERSION = \"`hg id -n`\" version.hh: FORCE if test -e ../.hg; \ then echo $(VERSION) | cmp -s $@ - || echo $(VERSION) > $@ ; \ fi FORCE: tantan-22/src/mcf_tantan_options.hh0000644000000000000000000000122113406427735016266 0ustar rootroot// Copyright 2010 Martin C. Frith #ifndef MCF_TANTAN_OPTIONS_HH #define MCF_TANTAN_OPTIONS_HH namespace mcf { struct TantanOptions { TantanOptions(); void fromArgs(int argc, char** argv); bool isProtein; char maskSymbol; bool isPreserveLowercase; const char *scoreMatrixFileName; double repeatProb; double repeatEndProb; int maxCycleLength; double repeatOffsetProbDecay; int matchScore; int mismatchCost; int gapExistenceCost; int gapExtensionCost; double minMaskProb; double minCopyNumber; enum OutputType { maskOut, probOut, countOut, bedOut, repOut } outputType; int indexOfFirstNonOptionArgument; }; } #endif tantan-22/src/LambdaCalculator.hh0000644000000000000000000000404013406427735015575 0ustar rootroot// Copyright 2008 Michiaki Hamada // Modified 2015 Yutaro Konta // This class calculates the scale factor (lambda), and the letter // probabilities, that are implicit in a scoring matrix. The // calculation might fail for various reasons, putting it into a // "bad/undefined" state. // If the score matrix is symmetric, then the two sets of letter // probabilities should be identical. With this code, they might // differ minutely from exact identity. #ifndef LAMBDA_CALCULATOR_HH #define LAMBDA_CALCULATOR_HH #include namespace cbrc{ typedef const int *const_int_ptr; class LambdaCalculator{ public: LambdaCalculator() { setBad(); } void calculate(const const_int_ptr *matrix, int alphSize); // Put us in the bad/undefined state. void setBad(); // Are we in the bad/undefined state? bool isBad() const { return (lambda_ < 0); } // The scale factor. In the bad/undefined state, it is negative. double lambda() const { return lambda_; } // The probabilities of letters corresponding to matrix rows (1st index). // In the bad/undefined state, it is a null pointer. const double *letterProbs1() const {return isBad() ? 0 : &letterProbs1_[0];} // The probabilities of letters corresponding to matrix columns (2nd index). // In the bad/undefined state, it is a null pointer. const double *letterProbs2() const {return isBad() ? 0 : &letterProbs2_[0];} private: double lambda_; std::vector letterProbs1_; std::vector letterProbs2_; bool find_ub(double **matrix, int alpha_size, double *ub); bool binary_search(double** matrix, int alpha_size, double lb, double ub, std::vector& letprob1, std::vector& letprob2, double* lambda, int maxiter); double calculate_lambda(double** matrix, int alpha_size, std::vector& letprob1, std::vector& letprob2, int maxiter, int max_boundary_search_iter, double lb_ratio); bool check_lambda(double** matrix, double lambda, int alpha_size, std::vector& letprob1, std::vector& letprob2); }; } // end namespace #endif tantan-22/src/version.hh0000644000000000000000000000000513406427735014065 0ustar rootroot"22" tantan-22/src/mcf_alphabet.cc0000644000000000000000000000421213406427735014777 0ustar rootroot// Copyright 2010 Martin C. Frith #include "mcf_alphabet.hh" #include // fill_n #include #include namespace mcf { // consts have internal linkage, apparently. // If I make these into static const class members, g++ 4.0.1 complains. const uchar defaultNumber = 255; const uchar defaultLetter = '!'; const char* Alphabet::dna = "ACGT"; // U=selenocysteine, O=pyrrolysine, *=stop? const char* Alphabet::protein = "ACDEFGHIKLMNPQRSTVWY"; void Alphabet::fromString(const std::string &normalLetters) { std::fill_n(lettersToNumbers, capacity, defaultNumber); std::fill_n(numbersToLetters, capacity, defaultLetter); uchar number = 0; addLetters(normalLetters, number); size = number; addLetters("ABCDEFGHIJKLMNOPQRSTUVWXYZ", number); addLetters("abcdefghijklmnopqrstuvwxyz", number); addLetters("*", number); // sometimes appears in protein sequences initCaseConversions(); } void Alphabet::addLetters(const std::string &letters, uchar &number) { for (unsigned i = 0; i < letters.size(); ++i) { uchar letter = static_cast(letters[i]); if (lettersToNumbers[letter] == defaultNumber) { lettersToNumbers[letter] = number; numbersToLetters[number] = letter; ++number; } } } void Alphabet::initCaseConversions() { for (unsigned i = 0; i < capacity; ++i) { uchar letter = numbersToLetters[i]; numbersToUppercase[i] = lettersToNumbers[std::toupper(letter)]; numbersToLowercase[i] = lettersToNumbers[std::tolower(letter)]; } } void Alphabet::makeCaseInsensitive() { for (unsigned i = 0; i < capacity; ++i) { lettersToNumbers[i] = lettersToNumbers[std::toupper(i)]; } } void Alphabet::encodeInPlace(uchar *beg, uchar *end) const { while (beg != end) { uchar letter = *beg; uchar number = lettersToNumbers[letter]; if (number == defaultNumber) { throw std::runtime_error(std::string("bad symbol: ") + char(letter)); } *beg = number; ++beg; } } void Alphabet::decodeInPlace(uchar *beg, uchar *end) const { while (beg != end) { uchar number = *beg; uchar letter = numbersToLetters[number]; *beg = letter; ++beg; } } } tantan-22/src/tantan.hh0000644000000000000000000001116413406427735013675 0ustar rootroot// Copyright 2010 Martin C. Frith // These are routines for masking simple regions (low-complexity and // short-period tandem repeats) in biological sequences. To // understand them in detail, see the published article (in // preparation). // Typically, you would just use the maskSequences routine. The other // routines are more specialized. The inputs to maskSequences are as // follows. // seqBeg: pointer to the start of the sequence. // seqEnd: pointer to one-past-the-end of the sequence. // maxRepeatOffset: the maximum tandem-repeat period-size to consider. // likelihoodRatioMatrix: a matrix of the form Qxy / (Px * Py), where // Qxy is the probability of seeing letters x and y in equivalent // positions of a tandem repeat, and Px and Py are the background // probabilities of the letters. This matrix is related to a scoring // matrix (e.g. blosum62) by: // likelihoodRatioMatrix[x][y] = exp(lambda * scoringMatrix[x][y]). // The uchars in the sequence will be used as indexes into the matrix // (e.g. likelihoodRatioMatrix[x][y]). So typically the uchars are // small integers (e.g. 0, 1, 2, 3). The matrix needs to have entries // for any uchars that can occur. // repeatProb: the probability of a repetitive segment starting per position. // repeatEndProb: the probability of a repetitive segment ending per position. // repeatOffsetProbDecay: the probability of a period-(i) repeat // divided by the probability of a period-(i-1) repeat. // firstGapProb: the probability of initiating an insertion or // deletion in a repetitive region. // otherGapProb: the probability of extending an insertion or deletion // by one more letter. // minMaskProb: mask letters whose posterior probability of being // repetitive is >= this. // maskTable: how to do the masking. Letter x will be changed to // maskTable[x]. So maskTable needs to have entries for any uchar // that can occur. // Typical usage: // tantan::maskSequences(seqBeg, seqEnd, 100, likelihoodRatioMatrix, // 0.005, 0.05, 0.9, 0, 0, 0.5, maskTable) #ifndef TANTAN_HH #define TANTAN_HH namespace tantan { typedef unsigned char uchar; typedef const double *const_double_ptr; void maskSequences(uchar *seqBeg, uchar *seqEnd, int maxRepeatOffset, const const_double_ptr *likelihoodRatioMatrix, double repeatProb, double repeatEndProb, double repeatOffsetProbDecay, double firstGapProb, double otherGapProb, double minMaskProb, const uchar *maskTable); // The following routine gets the posterior probability that each // letter is repetitive. It stores the results in "probabilities", // which must point to enough pre-allocated space to fit the results. void getProbabilities(const uchar *seqBeg, const uchar *seqEnd, int maxRepeatOffset, const const_double_ptr *likelihoodRatioMatrix, double repeatProb, double repeatEndProb, double repeatOffsetProbDecay, double firstGapProb, double otherGapProb, float *probabilities); // The following routine masks each letter whose corresponding entry // in "probabilities" is >= minMaskProb. void maskProbableLetters(uchar *seqBeg, uchar *seqEnd, const float *probabilities, double minMaskProb, const uchar *maskTable); // The following routine counts the expected number of transitions // from the background (non-repeat) state to other states. It adds // the results to "transitionCounts", which must point to // pre-initialized space for (maxRepeatOffset+1) items. The // background->background transition count is stored in // transitionCounts[0]. The background->(period-i repeat) transition // count is stored in transitionCounts[i]. // (In this routine, the HMM begin and end states are counted as // background states. Thus, begin->X is added to background->X, and // X->end is added to X->background.) void countTransitions(const uchar *seqBeg, const uchar *seqEnd, int maxRepeatOffset, const const_double_ptr *likelihoodRatioMatrix, double repeatProb, double repeatEndProb, double repeatOffsetProbDecay, double firstGapProb, double otherGapProb, double *transitionCounts); } #endif tantan-22/src/mcf_util.hh0000644000000000000000000000206313406427735014210 0ustar rootroot// Copyright 2010 Martin C. Frith #ifndef MCF_UTIL_HH #define MCF_UTIL_HH #include #include #include #include #include namespace mcf { // open an input file, but if the name is "-", just return cin std::istream &openIn(const std::string &fileName, std::ifstream &ifs); // open an output file, but if the name is "-", just return cout std::ostream &openOut(const std::string &fileName, std::ofstream &ofs); template std::string stringify(const T &x) { std::ostringstream oss; oss << x; assert(oss); return oss.str(); } template void unstringify(T& x, const std::string &s) { std::istringstream iss(s); if (!(iss >> x) || !(iss >> std::ws).eof()) throw std::runtime_error("can't interpret: " + s); } template void unfilify(T& x, const std::string &fileName) { std::ifstream ifs; std::istream &input = openIn(fileName, ifs); input >> x; if (!input) throw std::runtime_error("can't read file: " + fileName); // check for junk at end of file? } } #endif tantan-22/src/tantan_repeat_finder.hh0000644000000000000000000000457113406427735016570 0ustar rootroot// Copyright 2018 Martin C. Frith // This class finds tandem repeats in a sequence, using a Viterbi // algorithm. // The input parameters are as in tantan.hh, with one difference: the // substitutionMatrix should be a *log* likelihood ratio matrix: // substitutionMatrix[x][y] = lambda * scoringMatrix[x][y]. // Usage: first call init. Then call calcBestPathScore, which runs // the Viterbi algorithm backwards from the end to the start of the // sequence. Finally, call nextState once per sequence letter, to get // the "state" of each letter from start to end. // state = 0: non-repeat. // 0 < state <= maxRepeatOffset: tandem repeat with period = state. // maxRepeatOffset < state < 2*maxRepeatOffset: insertion in repeat. #ifndef TANTAN_REPEAT_FINDER_HH #define TANTAN_REPEAT_FINDER_HH #include namespace tantan { typedef unsigned char uchar; typedef const double *const_double_ptr; class RepeatFinder { public: void init(int maxRepeatOffset, const const_double_ptr *substitutionMatrix, double repeatProb, double repeatEndProb, double repeatOffsetProbDecay, double firstGapProb, double otherGapProb); double calcBestPathScore(const uchar *seqBeg, const uchar *seqEnd); int nextState(); private: const const_double_ptr *substitutionMatrix; double b2b; double f2b; double g2g; double oneGapScore; double endGapScore; double f2f0; double f2f1; double f2f2; double b2fGrowth; double b2fLast; int maxRepeatOffset; int dpScoresPerLetter; std::vector dpScores; double *scoresPtr; double *scoresEnd; double *checkpoint; const uchar *seqBeg; const uchar *seqEnd; const uchar *seqPtr; int state; void initializeBackwardScores(); void calcBackwardTransitionScoresWithGaps(); void calcBackwardTransitionScores(); void calcEmissionScores(); void makeCheckpoint(); void redoCheckpoint(); int offsetWithMaxScore() const; int deletionWithMaxScore() const; bool isNearSeqBeg() const { return seqPtr - seqBeg < maxRepeatOffset; } int maxOffsetInTheSequence() const { return isNearSeqBeg() ? (seqPtr - seqBeg) : maxRepeatOffset; } double scoreWithEmission(const double *matrixRow, int offset) const { return scoresPtr[offset] + matrixRow[seqPtr[-offset]]; } void calcScoresForOneSequencePosition() { calcEmissionScores(); calcBackwardTransitionScores(); } }; } #endif tantan-22/src/mcf_alphabet.hh0000644000000000000000000000246213406427735015016 0ustar rootroot// Copyright 2010 Martin C. Frith // An "Alphabet" consists of two things: // 1) A mapping from ASCII letters to small integers. // 2) A distinction between "normal" and "abnormal" letters. The // letters that map to the first "size" integers are normal. For // example, for DNA, ACGT would be normal. #ifndef MCF_ALPHABET_HH #define MCF_ALPHABET_HH #include namespace mcf { typedef unsigned char uchar; struct Alphabet { static const char* dna; static const char* protein; static const unsigned capacity = 256; // Initializes the Alphabet with the given normal letters. void fromString(const std::string &normalLetters); // case is preserved // Makes lowercase letters map to the same numbers that uppercase // letters do. void makeCaseInsensitive(); // Maps letters to numbers in the given range. void encodeInPlace(uchar *beg, uchar *end) const; // Maps numbers back to letters in the given range. void decodeInPlace(uchar *beg, uchar *end) const; unsigned size; // number of "normal" letters in the alphabet uchar lettersToNumbers[capacity]; uchar numbersToLetters[capacity]; uchar numbersToUppercase[capacity]; uchar numbersToLowercase[capacity]; private: void addLetters(const std::string &letters, uchar &number); void initCaseConversions(); }; } #endif tantan-22/src/mcf_score_matrix.hh0000644000000000000000000000372513406427735015740 0ustar rootroot// Copyright 2010 Martin C. Frith // This class reads and writes scoring matrices in NCBI BLAST format. // It can't be used directly: the idea is to copy it into a "fast" // matrix, and then use that. #ifndef MCF_SCORE_MATRIX_HH #define MCF_SCORE_MATRIX_HH #include #include #include namespace mcf { typedef unsigned char uchar; class ScoreMatrix { // These preserve (upper/lower)case of letters labeling rows & columns. friend std::istream &operator>>(std::istream &s, ScoreMatrix &m); friend std::ostream &operator<<(std::ostream &s, const ScoreMatrix &m); public: static const char* blosum62; // Initializes the matrix with simple match/mismatch scores. void initMatchMismatch(int matchScore, // usually > 0 int mismatchCost, // usually > 0 const std::string &letters); // case is preserved // Gets the minimum score in the matrix. int minScore() const; // Copies the ScoreMatrix into a "fast" matrix. // fastMatrixSize: the number of rows & columns in the fast matrix. // letterToIndex: mapping from letters, to indices in the fast // matrix. If any index is >= fastMatrixSize, an error is thrown. // defaultScore: for cells in the fast matrix that lack any // corresponding cell in the ScoreMatrix. // isCaseSensitive: if false, then each cell in the ScoreMatrix will // be copied to uppercase and lowercase locations in the fast // matrix. // It is possible that multiple ScoreMatrix cells might map to one // fast matrix cell: there is currently no check for this, and later // ones will overwrite earlier ones. void makeFastMatrix(int **fastMatrix, int fastMatrixSize, const uchar *letterToIndex, int defaultScore, bool isCaseSensitive) const; private: std::vector rowLetters; std::vector colLetters; std::vector< std::vector > scores; }; } #endif tantan-22/src/mcf_fasta_sequence.hh0000644000000000000000000000077313406427735016227 0ustar rootroot// Copyright 2010, 2011 Martin C. Frith #ifndef MCF_FASTA_SEQUENCE_HH #define MCF_FASTA_SEQUENCE_HH #include #include #include namespace mcf { typedef unsigned char uchar; struct FastaSequence { std::string title; std::vector sequence; // Used for fastq: std::string secondTitle; std::vector qualityCodes; }; std::istream &operator>>(std::istream &s, FastaSequence &f); std::ostream &operator<<(std::ostream &s, const FastaSequence &f); } #endif tantan-22/src/LambdaCalculator.cc0000644000000000000000000002140213406427735015564 0ustar rootroot// Copyright 2015 Yutaro Konta #include "LambdaCalculator.hh" #include #include #include // sprintf #include #include #include //#include using namespace std; static double roundToFewDigits(double x) { // This rounding fixes some inaccuracies, e.g. for DNA with a simple // match/mismatch matrix it's likely to make all the probabilities // exactly 0.25, as they should be. char buffer[32]; sprintf(buffer, "%g", x); return atof(buffer); } static double** makematrix(int m, int n, double val) { double** mat = new double* [m]; for (int i=0; i max) { max = fabs(a[j][i]); maxindex = j; } } return maxindex; } static void swap_matrix(double** a, int i, int j) { double* v = a[i]; a[i] = a[j]; a[j] = v; } static void swap_vector(int* a, int i, int j) { int v = a[i]; a[i] = a[j]; a[j] = v; } static bool lu_pivoting(double** a, int* idx, int n) { int v; for (int i=0; i= 0); if (fabs(a[v][i]) < 1e-10) { return false; // singular matrix! } swap_matrix(a, i, v); swap_vector(idx, i, v); a[i][i] = 1.0/a[i][i]; for (int j=i+1; j=0; i--) { x[i] = y[i]; for (int j=i+1; j r_max) r_max = matrix[i][j]; if (matrix[i][j] < r_min) r_min = matrix[i][j]; } if (r_max == 0 && r_min == 0) l_r++; else if (r_max <= 0 || r_min >= 0) return false; else if (r_max < r_max_min) r_max_min = r_max; } for (int j=0; j c_max) c_max = matrix[i][j]; if (matrix[i][j] < c_min) c_min = matrix[i][j]; } if (c_max == 0 && c_min == 0) l_c++; else if (c_max <= 0 || c_min >= 0) return false; else if (c_max < c_max_min) c_max_min = c_max; } if (l_r == alpha_size) return false; // the multiplication by 1.1 is sometimes necessary, presumably to // prevent the upper bound from being too tight: if (r_max_min > c_max_min) *ub = 1.1 * log(1.0 * (alpha_size - l_r))/r_max_min; else *ub = 1.1 * log(1.0 * (alpha_size - l_c))/c_max_min; return true; } bool LambdaCalculator::binary_search(double** matrix, int alpha_size, double lb, double ub, vector& letprob1, vector& letprob2, double* lambda, int maxiter) { double l=0; double r=0; double l_sum=0; double r_sum=0; int iter=0; while (iter < maxiter && (l>=r || (l_sum < 1.0 && r_sum < 1.0) || (l_sum > 1.0 && r_sum > 1.0))) { l = lb + (ub - lb)*rand()/RAND_MAX; r = lb + (ub - lb)*rand()/RAND_MAX; if (!calculate_inv_sum(matrix, alpha_size, l, &l_sum) || !calculate_inv_sum(matrix, alpha_size, r, &r_sum)) { l = 0; r = 0; } iter++; } if (iter >= maxiter) return false; while (l_sum != 1.0 && r_sum != 1.0 && (l+r)/2.0 != l && (l+r)/2.0 != r) { double mid = (l + r)/2.0; double mid_sum; if (!calculate_inv_sum(matrix, alpha_size, mid, &mid_sum)) return false; if (fabs(mid_sum) >= DBL_MAX) return false; if ((l_sum < 1.0 && mid_sum >= 1.0) || (l_sum > 1.0 && mid_sum <= 1.0)) { r = mid; r_sum = mid_sum; } else if ((r_sum < 1.0 && mid_sum >= 1.0) || (r_sum > 1.0 && mid_sum <= 1.0)) { l = mid; l_sum = mid_sum; } else return false; } if (fabs(l_sum - 1.0) < fabs(r_sum - 1.0)) { if (check_lambda(matrix, l, alpha_size, letprob1, letprob2)) { *lambda = l; return true; } return false; } if (check_lambda(matrix, r, alpha_size, letprob1, letprob2)) { *lambda = r; return true; } return false; } double LambdaCalculator::calculate_lambda(double** matrix, int alpha_size, vector& letprob1, vector& letprob2, int maxiter, int max_boundary_search_iter, double lb_ratio) { double ub; if (!find_ub(matrix, alpha_size, &ub)) return -1; double lb = ub*lb_ratio; double lambda = -1; int iter = 0; bool flag = false; while (!flag && iter < maxiter) { flag = binary_search(matrix, alpha_size, lb, ub, letprob1, letprob2, &lambda, max_boundary_search_iter); iter++; } return lambda; } bool LambdaCalculator::check_lambda(double** matrix, double lambda, int alpha_size, vector& letprob1, vector& letprob2) { double **m = makematrix(alpha_size, alpha_size, 0); double **y = makematrix(alpha_size, alpha_size, 0); for (int i=0; i 1) { letprob2.clear(); return false; } letprob2.push_back(roundToFewDigits(p)); } for (int j=0; j 1) { letprob2.clear(); letprob1.clear(); return false; } letprob1.push_back(roundToFewDigits(q)); } deletematrix(m, alpha_size); deletematrix(y, alpha_size); return true; } void LambdaCalculator::calculate(const const_int_ptr *matrix, int alphSize) { assert(alphSize >= 0); setBad(); int maxiter = 1000; int max_boundary_search_iter = 100; double lb_ratio = 1e-6; double** mat = makematrix(alphSize, alphSize, 0); for (int i=0; i #include #include #include //#include // for debugging namespace tantan { static double max3(double x, double y, double z) { return std::max(std::max(x, y), z); } static double myLog(double x) { return x > 0 ? log(x) : -HUGE_VAL; } static double firstRepeatOffsetProb(double probMult, int maxRepeatOffset) { if (probMult < 1 || probMult > 1) { return (1 - probMult) / (1 - pow(probMult, 1.0 * maxRepeatOffset)); } return 1.0 / maxRepeatOffset; } static int numOfDpScoresPerLetter(int maxRepeatOffset, double endGapScore) { if (endGapScore > -HUGE_VAL) { assert(maxRepeatOffset <= INT_MAX / 2); return maxRepeatOffset * 2; } assert(maxRepeatOffset < INT_MAX); return maxRepeatOffset + 1; } static unsigned minStoredPositions(const uchar *beg, const uchar *end) { // We will do a dynamic programming algorithm along the sequence. // To save memory, we will keep only some DP values, and recalculate // the others later. We keep the values in an array x of size s. // We will store DP initial values in x[0]. We will put // the DP values after the first s-1 sequence positions in x[1], // the DP values after the next s-2 positions in x[2], // the DP values after the next s-3 positions in x[3], etc. // This function returns the minimum possible value of s. unsigned t = 0; while (t < end - beg) { beg += t; ++t; } return t + 1; } void RepeatFinder::init(int maxRepeatOffset, const const_double_ptr *substitutionMatrix, double repeatProb, double repeatEndProb, double repeatOffsetProbDecay, double firstGapProb, double otherGapProb) { assert(maxRepeatOffset > 0); this->maxRepeatOffset = maxRepeatOffset; this->substitutionMatrix = substitutionMatrix; b2b = myLog(1 - repeatProb); f2b = myLog(repeatEndProb); g2g = myLog(otherGapProb); oneGapScore = myLog(firstGapProb * (1 - otherGapProb)); endGapScore = myLog(firstGapProb * (maxRepeatOffset > 1)); f2f0 = myLog(1 - repeatEndProb); f2f1 = myLog(1 - repeatEndProb - firstGapProb); f2f2 = myLog(1 - repeatEndProb - firstGapProb * 2); double x = 1 / repeatOffsetProbDecay; b2fGrowth = myLog(x); b2fLast = myLog(repeatProb * firstRepeatOffsetProb(x, maxRepeatOffset)); dpScoresPerLetter = numOfDpScoresPerLetter(maxRepeatOffset, endGapScore); } void RepeatFinder::initializeBackwardScores() { scoresPtr[0] = b2b; std::fill_n(scoresPtr + 1, maxRepeatOffset, f2b); if (endGapScore > -HUGE_VAL) { std::fill_n(scoresPtr + 1 + maxRepeatOffset, maxRepeatOffset-1, -HUGE_VAL); } } void RepeatFinder::calcBackwardTransitionScoresWithGaps() { double toBackground = f2b + scoresPtr[0]; double *foregroundPtr = scoresPtr + 1; double f = *foregroundPtr; double toForeground = f; double *insertionPtr = scoresPtr + 1 + maxRepeatOffset; double i = *insertionPtr; *foregroundPtr = max3(toBackground, f2f1 + f, i); double d = endGapScore + f; ++foregroundPtr; toForeground += b2fGrowth; while (foregroundPtr < scoresPtr + maxRepeatOffset) { f = *foregroundPtr; toForeground = std::max(toForeground, f); i = *(insertionPtr + 1); *foregroundPtr = max3(toBackground, f2f2 + f, std::max(i, d)); double oneGapScore_f = oneGapScore + f; *insertionPtr = std::max(oneGapScore_f, g2g + i); d = std::max(oneGapScore_f, g2g + d); ++foregroundPtr; ++insertionPtr; toForeground += b2fGrowth; } f = *foregroundPtr; toForeground = std::max(toForeground, f); *foregroundPtr = max3(toBackground, f2f1 + f, d); *insertionPtr = endGapScore + f; scoresPtr[0] = std::max(b2b + scoresPtr[0], b2fLast + toForeground); } void RepeatFinder::calcBackwardTransitionScores() { if (endGapScore > -HUGE_VAL) return calcBackwardTransitionScoresWithGaps(); double toBackground = f2b + scoresPtr[0]; double toForeground = -HUGE_VAL; double *foregroundPtr = scoresPtr + 1; double *foregroundEnd = foregroundPtr + maxRepeatOffset; while (foregroundPtr < foregroundEnd) { toForeground += b2fGrowth; double f = *foregroundPtr; toForeground = std::max(toForeground, f); *foregroundPtr = std::max(toBackground, f2f0 + f); ++foregroundPtr; } scoresPtr[0] = std::max(b2b + scoresPtr[0], b2fLast + toForeground); } void RepeatFinder::calcEmissionScores() { const double *matrixRow = substitutionMatrix[*seqPtr]; double *oldScores = scoresPtr - dpScoresPerLetter; int maxOffset = maxOffsetInTheSequence(); int i = 1; scoresPtr[0] = oldScores[0]; for (; i <= maxOffset; ++i) { scoresPtr[i] = oldScores[i] + matrixRow[seqPtr[-i]]; } for (; i <= maxRepeatOffset; ++i) { scoresPtr[i] = -HUGE_VAL; } std::copy(oldScores + i, scoresPtr, scoresPtr + i); } void RepeatFinder::makeCheckpoint() { checkpoint += dpScoresPerLetter; std::copy(scoresPtr - dpScoresPerLetter, scoresPtr, checkpoint); scoresPtr = checkpoint + dpScoresPerLetter; assert(scoresPtr < scoresEnd); } void RepeatFinder::redoCheckpoint() { seqPtr += (scoresEnd - scoresPtr) / dpScoresPerLetter; while (scoresPtr < scoresEnd) { --seqPtr; calcScoresForOneSequencePosition(); scoresPtr += dpScoresPerLetter; } scoresPtr -= dpScoresPerLetter; checkpoint -= dpScoresPerLetter; } double RepeatFinder::calcBestPathScore(const uchar *seqBeg, const uchar *seqEnd) { this->seqBeg = seqBeg; this->seqEnd = seqEnd; unsigned long numOfStoredPositions = minStoredPositions(seqBeg, seqEnd); unsigned long numOfScores = numOfStoredPositions * dpScoresPerLetter; assert(numOfStoredPositions > 0); assert(numOfScores > 0); dpScores.resize(numOfScores); scoresPtr = &dpScores[0]; scoresEnd = scoresPtr + numOfScores; checkpoint = scoresPtr; seqPtr = seqEnd; initializeBackwardScores(); while (seqPtr > seqBeg) { --seqPtr; scoresPtr += dpScoresPerLetter; if (scoresPtr == scoresEnd) makeCheckpoint(); calcScoresForOneSequencePosition(); } state = 0; return scoresPtr[0]; } int RepeatFinder::offsetWithMaxScore() const { const double *matrixRow = substitutionMatrix[*seqPtr]; int maxOffset = maxOffsetInTheSequence(); int bestOffset = 0; double toForeground = -HUGE_VAL; for (int i = 1; i <= maxOffset; ++i) { toForeground += b2fGrowth; double f = scoreWithEmission(matrixRow, i); if (f > toForeground) { toForeground = f; bestOffset = i; } } return bestOffset; } int RepeatFinder::deletionWithMaxScore() const { const double *matrixRow = substitutionMatrix[*seqPtr]; int bestOffset = 1; double f = scoreWithEmission(matrixRow, 1); double d = endGapScore + f; for (int i = 2; i < state; ++i) { d += g2g; f = scoreWithEmission(matrixRow, i); if (oneGapScore + f > d) { d = oneGapScore + f; bestOffset = i; } } return bestOffset; } int RepeatFinder::nextState() { double maxScore = scoresPtr[state]; if (scoresPtr == checkpoint) redoCheckpoint(); scoresPtr -= dpScoresPerLetter; if (state == 0) { if (b2b + scoresPtr[0] < maxScore) state = offsetWithMaxScore(); } else if (state <= maxRepeatOffset) { if (f2b + scoresPtr[0] >= maxScore) { state = 0; } else if (endGapScore > -HUGE_VAL) { double f = scoreWithEmission(substitutionMatrix[*seqPtr], state); if (state == 1) { if (f2f1 + f < maxScore) state += maxRepeatOffset; } else if (state == maxRepeatOffset) { if (f2f1 + f < maxScore) state = deletionWithMaxScore(); } else if (f2f2 + f < maxScore) { if (scoresPtr[state + maxRepeatOffset] >= maxScore) { state += maxRepeatOffset; } else { state = deletionWithMaxScore(); } } } } else { ++state; if (state == dpScoresPerLetter || g2g + scoresPtr[state] < maxScore) { state -= maxRepeatOffset; } } ++seqPtr; return state; } } tantan-22/src/mcf_fasta_sequence.cc0000644000000000000000000000565013406427735016214 0ustar rootroot// Copyright 2010, 2011 Martin C. Frith #include "mcf_fasta_sequence.hh" #include // isspace //#include // for debugging #include #include // istreambuf_iterator, ostreambuf_iterator #include namespace mcf { static void readSequence(std::istream &s, std::vector &sequence, char delimiter) { if (!s) return; std::istreambuf_iterator inpos(s); std::istreambuf_iterator endpos; while (inpos != endpos) { char c = *inpos; if (c == delimiter) break; uchar u = static_cast(c); if (!std::isspace(u)) sequence.push_back(u); ++inpos; } } static void readQualityCodes(std::istream &s, std::vector &qualityCodes, std::vector::size_type howMany) { if (!s) return; std::istreambuf_iterator inpos(s); std::istreambuf_iterator endpos; while (inpos != endpos) { if (qualityCodes.size() == howMany) break; char c = *inpos; uchar u = static_cast(c); if (!std::isspace(u)) qualityCodes.push_back(u); ++inpos; } } std::istream &operator>>(std::istream &s, FastaSequence &f) { std::string title; std::vector sequence; std::string secondTitle; std::vector qualityCodes; char firstChar = '>'; s >> firstChar; if (firstChar != '>' && firstChar != '@') s.setstate(std::ios::failbit); getline(s, title); if (firstChar == '>') { readSequence(s, sequence, '>'); } else { readSequence(s, sequence, '+'); char secondChar; s >> secondChar; getline(s, secondTitle); readQualityCodes(s, qualityCodes, sequence.size()); // perhaps check whether we read enough quality codes } if (!s) return s; f.title.swap(title); f.sequence.swap(sequence); f.secondTitle.swap(secondTitle); f.qualityCodes.swap(qualityCodes); return s; } static void writeOneLine(std::ostream &s, const std::vector &v) { std::ostreambuf_iterator o(s); std::vector::const_iterator b = v.begin(); std::vector::const_iterator e = v.end(); while (b != e) { o = static_cast(*b); ++b; } o = '\n'; } static void writeMultiLines(std::ostream &s, const std::vector &v) { const int lettersPerLine = 50; // ? std::ostreambuf_iterator o(s); std::vector::const_iterator b = v.begin(); std::vector::const_iterator e = v.end(); std::vector::const_iterator i = b; while (i != e) { o = static_cast(*i); ++i; if (i - b == lettersPerLine || i == e) { o = '\n'; b = i; } } } std::ostream &operator<<(std::ostream &s, const FastaSequence &f) { if (f.qualityCodes.empty()) { s << '>' << f.title << '\n'; writeMultiLines(s, f.sequence); } else { s << '@' << f.title << '\n'; writeOneLine(s, f.sequence); s << '+' << f.secondTitle << '\n'; writeOneLine(s, f.qualityCodes); } return s; } } tantan-22/README.html0000644000000000000000000004144213406427735013125 0ustar rootroot tantan

tantan

Introduction

tantan is a tool for masking simple regions (low complexity and short-period tandem repeats) in biological sequences.

The aim of tantan is to prevent false predictions when searching for homologous regions between two sequences. Simple repeats often align strongly to each other, causing false homology predictions.

Setup

You need to have a C++ compiler. On Linux, you might need to install a package called "g++". On Mac, you might need to install command-line developer tools. On Windows, you might need to install Cygwin.

Using the command line, go into the tantan directory. To compile it, type:

make

Optionally, copy tantan to a standard "bin" directory (using "sudo" to request administrator permissions):

sudo make install

Or copy it to your personal bin directory:

make install prefix=~

You might need to log out and back in for your computer to recognize the new program.

Normal usage

  • Suppose you have some nucleotide sequences (DNA or RNA) in a FASTA-format file called "ntseqs.fa". You can identify simple repeats like this:

    tantan ntseqs.fa > masked.fa

    This will create a new FASTA file called "masked.fa" that replaces all masked regions with lowercase letters. (tantan also works on FASTQ-format, though it does not use the quality data.)

  • To mask proteins effectively, tantan needs to use different algorithm parameters than for nucleotides. You have to tell it when you have proteins, using the "-p" option:

    tantan -p aaseqs.fa > masked.fa

    If you omit "-p" and the sequences look proteinaceous, tantan will print a warning message.

Advanced usage

  • By default, tantan indicates repetitive regions with lowercase letters. You can make it replace repetitive letters with (say) "N" by using the "-x" option:

    tantan -x N ntseqs.fa > masked.fa

  • By default, tantan does not preserve lowercase letters in the input sequences. You can tell it to preserve them by using the "-c" option. So the output will have the union of the lowercase in the input and the lowercase assigned by tantan:

    tantan -c ntseqs.fa > masked.fa

  • tantan's masking rate is usually OK, but you can alter it by changing the "-r" parameter from its default value of 0.005. Higher values increase the amount of masking, and lower values decrease it. This increases the masking rate:

    tantan -r 0.02 ntseqs.fa > masked.fa

  • Finally, to mask extremely AT-rich DNA, you should change tantan's scoring matrix. The "test" directory contains a matrix "atMask.mat" that works well for DNA with ~80% A+T, such as Plasmodium and Dictyostelium genomes. We recommend masking such DNA like this:

    tantan -m atMask.mat -r 0.01 atrich.fa > masked.fa

The preceding examples cover all of tantan's options that you should ever need.

FAQ

Why does tantan sometimes mask isolated bases?

This happens when a region is borderline repetitive, and a single base creeps just over the threshold. Don't worry about it, it's not a problem (at least for tantan's aim of preventing false homology).

Does tantan mask functional sequence?

Of course. Proteins and protein-coding exons can contain simple repeats. Repeats can be functional. If we want to avoid false homology we have to mask them. Remember that tantan merely lowercases repeats, so it's easy to lift the masking after determining homology.

Options

-p interpret the sequences as proteins
-x letter to use for masking, instead of lowercase
-c preserve uppercase/lowercase in non-masked regions
-m file for letter-pair score matrix
-r probability of a repeat starting per position
-e probability of a repeat ending per position
-w maximum tandem repeat period to consider
-d probability decay per period (period-(i+1) / period-i)
-i match score
-j mismatch cost
-a gap existence cost
-b gap extension cost
-s minimum repeat probability for masking
-n minimum copy number, affects -f4 only
-f output type: 0=masked sequence, 1=repeat probabilities, 2=repeat counts, 3=BED, 4=tandem repeats
-h, --help show help message, then exit
--version show version information, then exit

Advanced issues

When tantan masks tandem repeats, it tends to leave the first (left-most) repeat unit unmasked. This sometimes allows us to find homologs we would otherwise miss:

TGCAAGCTA TTAGGCTTAGGTCAGTGC ttaagcttaggtcagtgc AACATA
||| ||| | |||||||||||||||||| ||| |||||||||||||| ||| ||
TGCTAGCAA TTAGGCTTAGGTCAGTGC ttaggcttaggtcagtgc AACGTA

However, there is a danger of non-equivalent repeat units being unmasked. This happens especially if we mask DNA on one strand but align it on the other strand:

                   TGCAAGCTA TTAGGCTTAGGTCAGTGC ttaagcttaggtcagtgc AACATA
                             ||||||||||||||||||
TGCTAGCAA ttaggcttaggtcagtgc TTAGGCTTAGGTCAGTGC AACGTA

(My thanks to Junko Tsuji and Paul Horton for finding these issues.)

Finding straightforward tandem repeats

Option -f4 runs tantan in a different mode, where it finds straightforward tandem repeats only. (Technically, it uses a Viterbi algorithm instead of a Forward-Backward algorithm.) This is not recommended for avoiding false homologs! But it might be useful for studying tandem repeats. The output looks like this:

mySeq   14765   14780   6       2.5     GTCATG  GTCATG,GTCATG,GTC
mySeq   632362  632377  2       6       GC      GC,GC,GC,GCt,GCT,GCT
mySeq   1278353 1278369 3       6.5     TCA     TCA,TCA,TCA,TC-,TC,TC,T
mySeq   3616084 3616100 3       5.33333 TGG     TGA,TGA,TGG,TGG,TGG,T

The first 3 columns show the start and end coordinates of the repetitive region, in BED format. Column 4 shows the length of the repeating unit (which might vary due to insertions and deletions, so this column shows the most common length). Column 5 shows the number of repeat units. Column 6 shows the repeating unit (which again might vary, so this is just a representative). Column 7 shows the whole repeat: lowercase letters are insertions relative to the previous repeat unit, and dashes are deletions relative to the previous repeat unit.

Miscellaneous

tantan is distributed under the GNU General Public License, either version 3 of the License, or (at your option) any later version. For details, see COPYING.txt.

If you use tantan in your research, please cite: "A new repeat-masking method enables specific detection of homologous sequences", MC Frith, Nucleic Acids Research 2011 39(4):e23.

tantan's website is: http://www.cbrc.jp/tantan/

If you have any questions, comments, or problems concerning tantan, please email: tantan (ATmark) cbrc (dot) jp.

tantan-22/ChangeLog.txt0000644000000000000000000000721613406427735013673 0ustar rootroot2018-12-19 Martin C. Frith * src/tantan.cc: Make it faster [3523060bcfb9] [tip] * src/tantan_repeat_finder.cc, src/tantan_repeat_finder.hh: Make -f4 a bit faster [1fccdf6e21be] * README.txt, src/mcf_tantan_options.cc, src/mcf_tantan_options.hh, src/tantan_app.cc, src/tantan_repeat_finder.cc, src/tantan_repeat_finder.hh, test/hard.fa, test/tantan_test.out, test/tantan_test.sh: Add option to find straightforward tandem repeats [1bae60144712] * README.txt, src/mcf_tantan_options.cc, src/tantan.cc, src/tantan_app.cc: Tweak the help message [fc1ca32a72aa] 2018-12-10 Martin C. Frith * README.txt, src/mcf_tantan_options.cc, src/mcf_tantan_options.hh, src/tantan_app.cc, test/tantan_test.out, test/tantan_test.sh: Add match score, mismatch cost options [2383404c795a] * src/tantan.cc: Refactor [d61af119db30] * src/tantan.cc: Refactor [81bdfe23217d] * src/CA_code/lambda_calculator.c, src/CA_code/lambda_calculator.h, src/CA_code/lubksb.c, src/CA_code/ludcmp.c, src/CA_code/nrutil.c, src/CA_code/nrutil.h, src/LambdaCalculator.cc, src/LambdaCalculator.hh, src/Makefile, src/mcf_score_matrix_probs.cc, src/mcf_score_matrix_probs.hh, src/tantan_app.cc: Use Konta-san's code for matrix lambda [fccc8e5e9c1b] * src/tantan.cc, src/tantan_app.cc, test/tantan_test.sh: Refactor [f9a9da99553d] 2012-10-16 Martin C. Frith * README.txt: Expanded the documentation: installation & FAQ [946a951b1a06] * src/CA_code/nrutil.c: Just fixed a compiler warning [fa79ce69a581] 2012-08-08 Martin C. Frith * Makefile: Avoid getting extra, unwanted files in the zip archive [d1d55af0e693] * test/tantan_test.sh: Made the test script less likely to use an unexpected version of tantan [894f9fc63c3e] * Makefile, README.txt: Added "make install", and HTML-ization of the README [674f2f7da185] * src/Makefile: Made the Makefile check the version number [ea0be7d72912] * README.txt: Converted the README to reStructuredText [25b631ab86b4] * src/Makefile: Generalized the Makefile [9c324ee14279] 2011-07-07 Martin C. Frith * README.txt: Just changed the documentation a bit [2d6dfe34fe33] 2011-03-22 Martin C. Frith * README.txt, src/mcf_tantan_options.cc, src/mcf_tantan_options.hh, src/tantan_app.cc: Added BED output option. Clarified the README (thanks: Joe Ryan). [db4af8e37a20] 2011-01-07 Martin C. Frith * README.txt, src/mcf_fasta_sequence.cc, src/mcf_fasta_sequence.hh, test/panda.fastq, test/tantan_test.out, test/tantan_test.sh: Made tantan work on fastq format, and improved the README [e7d454a5982d] 2010-11-16 Martin C. Frith * README.txt, src/Makefile, src/mcf_alphabet.cc, src/mcf_alphabet.hh, src/mcf_tantan_options.cc: Fixed compile error with g++ 4.0.1 [97a2b94e5835] 2010-10-13 Martin C. Frith * src/tantan_app.cc: Boost the version number to 1 [c5a20b01a563] * COPYING.txt, README.txt, src/CA_code/lambda_calculator.c, src/CA_code/lambda_calculator.h, src/CA_code/lubksb.c, src/CA_code/ludcmp.c, src/CA_code/nrutil.c, src/CA_code/nrutil.h, src/Makefile, src/mcf_alphabet.cc, src/mcf_alphabet.hh, src/mcf_fasta_sequence.cc, src/mcf_fasta_sequence.hh, src/mcf_score_matrix.cc, src/mcf_score_matrix.hh, src/mcf_score_matrix_probs.cc, src/mcf_score_matrix_probs.hh, src/mcf_tantan_options.cc, src/mcf_tantan_options.hh, src/mcf_util.cc, src/mcf_util.hh, src/tantan.cc, src/tantan.hh, src/tantan_app.cc, test/atMask.mat, test/hg19_chrM.fa, test/tantan_test.out, test/tantan_test.sh, test/titin_human.fa: First commit [4f9b0ba31588] tantan-22/COPYING.txt0000644000000000000000000010451313406427735013152 0ustar rootroot GNU GENERAL PUBLIC LICENSE Version 3, 29 June 2007 Copyright (C) 2007 Free Software Foundation, Inc. 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IN NO EVENT UNLESS REQUIRED BY APPLICABLE LAW OR AGREED TO IN WRITING WILL ANY COPYRIGHT HOLDER, OR ANY OTHER PARTY WHO MODIFIES AND/OR CONVEYS THE PROGRAM AS PERMITTED ABOVE, BE LIABLE TO YOU FOR DAMAGES, INCLUDING ANY GENERAL, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES ARISING OUT OF THE USE OR INABILITY TO USE THE PROGRAM (INCLUDING BUT NOT LIMITED TO LOSS OF DATA OR DATA BEING RENDERED INACCURATE OR LOSSES SUSTAINED BY YOU OR THIRD PARTIES OR A FAILURE OF THE PROGRAM TO OPERATE WITH ANY OTHER PROGRAMS), EVEN IF SUCH HOLDER OR OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. 17. Interpretation of Sections 15 and 16. If the disclaimer of warranty and limitation of liability provided above cannot be given local legal effect according to their terms, reviewing courts shall apply local law that most closely approximates an absolute waiver of all civil liability in connection with the Program, unless a warranty or assumption of liability accompanies a copy of the Program in return for a fee. END OF TERMS AND CONDITIONS How to Apply These Terms to Your New Programs If you develop a new program, and you want it to be of the greatest possible use to the public, the best way to achieve this is to make it free software which everyone can redistribute and change under these terms. To do so, attach the following notices to the program. It is safest to attach them to the start of each source file to most effectively state the exclusion of warranty; and each file should have at least the "copyright" line and a pointer to where the full notice is found. Copyright (C) This program is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this program. If not, see . Also add information on how to contact you by electronic and paper mail. If the program does terminal interaction, make it output a short notice like this when it starts in an interactive mode: Copyright (C) This program comes with ABSOLUTELY NO WARRANTY; for details type `show w'. This is free software, and you are welcome to redistribute it under certain conditions; type `show c' for details. The hypothetical commands `show w' and `show c' should show the appropriate parts of the General Public License. Of course, your program's commands might be different; for a GUI interface, you would use an "about box". You should also get your employer (if you work as a programmer) or school, if any, to sign a "copyright disclaimer" for the program, if necessary. For more information on this, and how to apply and follow the GNU GPL, see . The GNU General Public License does not permit incorporating your program into proprietary programs. If your program is a subroutine library, you may consider it more useful to permit linking proprietary applications with the library. If this is what you want to do, use the GNU Lesser General Public License instead of this License. But first, please read .